Q91VR5 · DDX1_MOUSE

  • Protein
    ATP-dependent RNA helicase DDX1
  • Gene
    Ddx1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation (By similarity).
Acts as a positive transcriptional regulator of cyclin CCND2 expression (PubMed:19398953).
Binds to the cyclin CCND2 promoter region (PubMed:19398953).
Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (By similarity).
Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541).
Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (PubMed:21703541).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site46-53ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcleavage body
Cellular Componentcytoplasm
Cellular Componentcytoplasmic stress granule
Cellular Componentcytosol
Cellular Componentmitochondrion
Cellular Componentnucleus
Cellular Componentribonucleoprotein complex
Cellular ComponenttRNA-splicing ligase complex
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionchromatin binding
Molecular FunctionDNA binding
Molecular FunctionDNA/RNA helicase activity
Molecular Functiondouble-stranded RNA binding
Molecular Functionexonuclease activity
Molecular Functionnuclease activity
Molecular Functionpoly(A) binding
Molecular FunctionRNA helicase activity
Molecular Functiontranscription coregulator activity
Biological Processdefense response to virus
Biological ProcessDNA duplex unwinding
Biological Processdouble-strand break repair
Biological Processinnate immune response
Biological ProcessmRNA processing
Biological Processpositive regulation of canonical NF-kappaB signal transduction
Biological Processpositive regulation of myeloid dendritic cell cytokine production
Biological Processprotein localization to cytoplasmic stress granule
Biological Processresponse to exogenous dsRNA
Biological Processresponse to virus
Biological ProcesstRNA splicing, via endonucleolytic cleavage and ligation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent RNA helicase DDX1
  • EC number
  • Alternative names
    • DEAD box protein 1

Gene names

    • Name
      Ddx1

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N-3
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q91VR5
  • Secondary accessions
    • Q3TU41

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm, cytosol
Cytoplasm
Cytoplasmic granule
Mitochondrion
Note: Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (By similarity).
Colocalizes in the cytosol with DDX21, DHX36 and TICAM1 (PubMed:21703541).
Colocalizes in the mitochondria with TICAM1 and poly(I:C) RNA ligand (PubMed:21703541).
The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) stimulation (PubMed:21703541).

Keywords

PTM/Processing

Features

Showing features for chain, modified residue, cross-link.

TypeIDPosition(s)Description
ChainPRO_00000549871-740ATP-dependent RNA helicase DDX1
Modified residue239N6-acetyllysine
Modified residue268N6-acetyllysine
Modified residue281N6-acetyllysine; alternate
Cross-link281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate
Modified residue481Phosphoserine

Post-translational modification

Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).

Keywords

Proteomic databases

2D gel databases

PTM databases

Expression

Tissue specificity

Testis-specific. Expressed in the germ line stem cells, spermatogonia and spermatocytes of the testis. Also expressed in the seminoma and nonseminoma types of testicular germ cell tumors (TGCTs) (at protein level).

Developmental stage

Expressed in the testis from 11.5 to 19.5 dpc.

Gene expression databases

Interaction

Subunit

Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without poly(I:C) RNA ligand stimulation (PubMed:21703541).
Interacts with DHX36 (PubMed:21703541).
Interacts (via B30.2/SPRY domain) with DDX21 (via N-terminus); this interaction serves as bridges to TICAM1 (PubMed:21703541).
Interacts with FAM98A (via N- and C-terminus) (By similarity).
Interacts with MBNL1 (By similarity).
Interacts with CSTF2 (By similarity).
Interacts with HNRNPK (By similarity).
Interacts with ATM (By similarity).
Interacts with RELA (via C-terminus) (By similarity).
Component of the tRNA-splicing ligase complex (By similarity).
Interacts with PHF5A (via C-terminus) (PubMed:18758164).
Interacts with PQBP1 (By similarity).
Interacts with ERCC6 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, domain, motif.

TypeIDPosition(s)Description
Region1-295Necessary for interaction with HNRNPK
Region1-448Interaction with dsRNA
Region1-525Necessary for interaction with RELA
Domain2-428Helicase ATP-binding
Domain70-247B30.2/SPRY
Motif370-373DEAD box
Domain493-681Helicase C-terminal
Region525-740Necessary for interaction with HNRNPK

Domain

The helicase domain is involved in the stimulation of RELA transcriptional activity.

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    740
  • Mass (Da)
    82,500
  • Last updated
    2001-12-01 v1
  • Checksum
    76457FBB75A3CEC2
MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRGTRGLLKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPAHIKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDNYIVKSQHTGNAQVSQTKFLPNAPKALIVEPSRELAEQTLNNVKQFKKYIDNPKLRELLIIGGVAARDQLSVLDNGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQITCDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDKLWERLGKNHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSNRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGNYKGHVDVLAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1Y7VM48A0A1Y7VM48_MOUSEDdx1190

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK028341
EMBL· GenBank· DDBJ
BAC25893.1
EMBL· GenBank· DDBJ
mRNA
AK153335
EMBL· GenBank· DDBJ
BAE31914.1
EMBL· GenBank· DDBJ
mRNA
AK160982
EMBL· GenBank· DDBJ
BAE36130.1
EMBL· GenBank· DDBJ
mRNA
BC010624
EMBL· GenBank· DDBJ
AAH10624.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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