Q91DD9 · VP40_EBORE

Function

function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis).

Miscellaneous

Most abundant protein in the virion.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componenthost cell endomembrane system
Cellular Componenthost cell late endosome membrane
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Cellular Componentribonucleoprotein complex
Cellular Componentvirion membrane
Molecular FunctionRNA binding
Molecular Functionstructural constituent of virion
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processviral budding via host ESCRT complex
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Matrix protein VP40
  • Alternative names
    • Ebola VP40
      (eVP40
      )
    • Membrane-associated protein VP40

Gene names

    • Name
      VP40

Organism names

Accessions

  • Primary accession
    Q91DD9

Proteomes

Subcellular Location

Virion membrane
; Peripheral membrane protein
Host late endosome membrane
; Peripheral membrane protein
Host cell membrane
; Peripheral membrane protein
Host endomembrane system
; Peripheral membrane protein
Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002450801-331Matrix protein VP40

Interaction

Subunit

Homodimer. Homohexamer. Homooctamer. Exists as a dimer until it reorganizes at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Hexamers are critical for budding. Octamers function in genome replication and RNA binding. Interacts with host TSG101. As a homohexamer, interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host PDCD6IP/ALIX; this interaction supports efficient egress of viral particles. Interacts with VP35. Interacts with host ITCH; this interaction is required for efficient egress. Interacts (via PPXY motif) with host SMURF2 (via WW domains); the interaction positively regulates virus budding.

Structure

3D structure databases

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif7-10PTAP/PSAP motif
Motif10-13PPXY motif
Region212-214Important for oligomerization
Region213-326Membrane-binding

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and the three WW domains of SMURF2 E3 ubiquitin ligase.

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    331
  • Mass (Da)
    35,863
  • Last updated
    2001-12-01 v1
  • Checksum
    C5FEB11D60218C20
MRRGVLPTAPPAYNDIAYSMSILPTRPSVIVNETKSDVLAVPGADVPSNSMRPVADDNIDHSSHTPSGVASAFILEAKVNVISGTKVLMKQIPIWLPLGVADQKIYSFDSTTAAIMLASYTVTHFGKISNPLVRVNRLGPGIPDHPLRLLRLGNQAFLQEFVLPPVQLPQYFTFDLTALKLITQPLPAATWTDETPAGAVNALRPGLSLHPKLRPILLPGKIGKKGHASDLTSPDKIQTIMNAIPDLKIVPIDPIKNIVGIEVPELLVQRLTGKKPQPKNGQPIIPVLLPKYVGLDPISPGDLTMVITQDCDSCHSPASHPYHMDKQDSYQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB050936
EMBL· GenBank· DDBJ
BAB69005.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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