Q91610 · DHHA_XENLA
- ProteinDesert hedgehog protein A
- Genedhh-a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids396 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Desert hedgehog protein A
The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity).
Both activities result in the cleavage of the full-length protein into two parts (DhhN and DhhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated DhhN (By similarity).
Both activities occur in the reticulum endoplasmic (By similarity).
Both activities result in the cleavage of the full-length protein into two parts (DhhN and DhhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated DhhN (By similarity).
Both activities occur in the reticulum endoplasmic (By similarity).
Desert hedgehog protein A N-product
The dually lipidated desert hedgehog protein N-product (DhhNp) is essential for a variety of patterning events during development (By similarity).
Involved in the early induction and patterning of anterodorsal ectoderm, nervous system and somites. Induces ectopic cement gland formation in embryos (PubMed:7671800).
Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity).
Involved in the early induction and patterning of anterodorsal ectoderm, nervous system and somites. Induces ectopic cement gland formation in embryos (PubMed:7671800).
Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity).
Catalytic activity
Desert hedgehog protein A
cholesterol + glycyl-L-cysteinyl-[protein] + H+ = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 89 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 90 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 90 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 95 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 125 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 126 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 126 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 129 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 131 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 140 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 147 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 182 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 197-198 | Cleavage; by autolysis | ||||
Sequence: GC | ||||||
Site | 267 | Involved in auto-cleavage | ||||
Sequence: T | ||||||
Site | 270 | Essential for auto-cleavage | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | extracellular region | |
Cellular Component | Golgi membrane | |
Cellular Component | plasma membrane | |
Molecular Function | cholesterol-protein transferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | patched binding | |
Molecular Function | peptidase activity | |
Biological Process | cell-cell signaling | |
Biological Process | intein-mediated protein splicing | |
Biological Process | positive regulation of smoothened signaling pathway | |
Biological Process | protein autoprocessing | |
Biological Process | self proteolysis | |
Biological Process | system development |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDesert hedgehog protein A
- EC number
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionQ91610
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Desert hedgehog protein A N-product
Cell membrane ; Lipid-anchor
Desert hedgehog protein A
Note: Co-localizes with HHAT in the ER and Golgi membrane.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MPAVRIVILAICCGLLLVPVRC | ||||||
Lipidation | 23 | N-palmitoyl cysteine | ||||
Sequence: C | ||||||
Chain | PRO_0000013251 | 23-197 | Desert hedgehog protein A N-product | |||
Sequence: CGPGRGPVGRRRYMRKLVPLHYKQFVPNVPEKTLGASGKSEGKIHRGSERFIELVPNYNPDIIFKDEEKTGADRLMTERCKDRVNALAISVMNMWPGVKLRVTEGWDEDGHHAHDSLHYEGRALDITTSDRDRNKYGMLARLAVEAGFDWVYYESKAHIHVSVKADNSLGVRSGG | ||||||
Chain | PRO_0000013250 | 23-396 | Desert hedgehog protein A | |||
Sequence: CGPGRGPVGRRRYMRKLVPLHYKQFVPNVPEKTLGASGKSEGKIHRGSERFIELVPNYNPDIIFKDEEKTGADRLMTERCKDRVNALAISVMNMWPGVKLRVTEGWDEDGHHAHDSLHYEGRALDITTSDRDRNKYGMLARLAVEAGFDWVYYESKAHIHVSVKADNSLGVRSGGCFPGTAMVMMGTGERKPLSELKIGDTVYTTDETGQLITSVVLLFLHRNPYKTATFVLIEAEGHPSKLLVTPNHLLFIQSSSSAGFLPTFAYRVQIGDLVQIYVNGTQVQSSKVVRVSLEEQTGVYAPMTEHGTLLVDGVLTSCYATVESHTLAHVSLAPLRLFQGIASMLPDLDMSDGVHWYCHILYVLAKYVLWWDMP | ||||||
Lipidation | 197 | Cholesterol glycine ester | ||||
Sequence: G |
Post-translational modification
Desert hedgehog protein A
Partially autoproteolyzed (By similarity).
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity (By similarity).
Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (DhhN) (By similarity).
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity (By similarity).
Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (DhhN) (By similarity).
Desert hedgehog protein A N-product
N-palmitoylation by HHAT of DhhN is required for desert hedgehog protein N-product multimerization and full activity (By similarity).
Keywords
- PTM
Expression
Tissue specificity
Expressed in the marginal zone at early gastrulation. In the neurula, expression is restricted to anterior structures, encompassing both neural plate and endodermal cells. Expressed on the inner surface of the pharynx at the early tadpole stage.
Developmental stage
First expressed during early gastrulation. Expression peaks during neural induction and early organogenesis.
Gene expression databases
Interaction
Subunit
Desert hedgehog protein A N-product
Multimer.
Interacts with BOC and CDON. Interacts with HHIP.
Structure
Family & Domains
Domain
Desert hedgehog protein A N-product
Binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.
Desert hedgehog protein A
The C-terminal domain regulates the auto-processing and controls the juxtacrine signaling.
Sequence similarities
Belongs to the hedgehog family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length396
- Mass (Da)44,087
- Last updated1996-11-01 v1
- Checksum774A3EC2268A5EE9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U26349 EMBL· GenBank· DDBJ | AAA85163.1 EMBL· GenBank· DDBJ | mRNA |