Q91610 · DHHA_XENLA

Function

function

Desert hedgehog protein A

The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity).
Both activities result in the cleavage of the full-length protein into two parts (DhhN and DhhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated DhhN (By similarity).
Both activities occur in the reticulum endoplasmic (By similarity).

Desert hedgehog protein A N-product

The dually lipidated desert hedgehog protein N-product (DhhNp) is essential for a variety of patterning events during development (By similarity).
Involved in the early induction and patterning of anterodorsal ectoderm, nervous system and somites. Induces ectopic cement gland formation in embryos (PubMed:7671800).
Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (By similarity).

Catalytic activity

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site89Ca2+ 1 (UniProtKB | ChEBI)
Binding site90Ca2+ 1 (UniProtKB | ChEBI)
Binding site90Ca2+ 2 (UniProtKB | ChEBI)
Binding site95Ca2+ 1 (UniProtKB | ChEBI)
Binding site125Ca2+ 1 (UniProtKB | ChEBI)
Binding site126Ca2+ 1 (UniProtKB | ChEBI)
Binding site126Ca2+ 2 (UniProtKB | ChEBI)
Binding site129Ca2+ 2 (UniProtKB | ChEBI)
Binding site131Ca2+ 2 (UniProtKB | ChEBI)
Binding site140Zn2+ (UniProtKB | ChEBI)
Binding site147Zn2+ (UniProtKB | ChEBI)
Binding site182Zn2+ (UniProtKB | ChEBI)
Site197-198Cleavage; by autolysis
Site267Involved in auto-cleavage
Site270Essential for auto-cleavage

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentextracellular region
Cellular ComponentGolgi membrane
Cellular Componentplasma membrane
Molecular Functioncholesterol-protein transferase activity
Molecular Functionmetal ion binding
Molecular Functionpatched binding
Molecular Functionpeptidase activity
Biological Processcell-cell signaling
Biological Processintein-mediated protein splicing
Biological Processpositive regulation of smoothened signaling pathway
Biological Processprotein autoprocessing
Biological Processself proteolysis
Biological Processsystem development

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

Gene names

    • Name
      dhh-a
    • Synonyms
      chh

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    Q91610

Proteomes

Organism-specific databases

Subcellular Location

Desert hedgehog protein A N-product

Cell membrane
; Lipid-anchor

Desert hedgehog protein A

Secreted
Cell membrane
Note: Co-localizes with HHAT in the ER and Golgi membrane.

Keywords

PTM/Processing

Features

Showing features for signal, lipidation, chain.

TypeIDPosition(s)Description
Signal1-22
Lipidation23N-palmitoyl cysteine
ChainPRO_000001325123-197Desert hedgehog protein A N-product
ChainPRO_000001325023-396Desert hedgehog protein A
Lipidation197Cholesterol glycine ester

Post-translational modification

Desert hedgehog protein A

Partially autoproteolyzed (By similarity).
The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity (By similarity).
Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (DhhN) (By similarity).

Desert hedgehog protein A N-product

N-palmitoylation by HHAT of DhhN is required for desert hedgehog protein N-product multimerization and full activity (By similarity).

Keywords

Expression

Tissue specificity

Expressed in the marginal zone at early gastrulation. In the neurula, expression is restricted to anterior structures, encompassing both neural plate and endodermal cells. Expressed on the inner surface of the pharynx at the early tadpole stage.

Developmental stage

First expressed during early gastrulation. Expression peaks during neural induction and early organogenesis.

Gene expression databases

    • 378538Expressed in camera-type eye and 13 other cell types or tissues

Interaction

Subunit

Desert hedgehog protein A N-product

Multimer.
Interacts with BOC and CDON. Interacts with HHIP.

Structure

Family & Domains

Domain

Desert hedgehog protein A N-product

Binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.

Desert hedgehog protein A

The C-terminal domain regulates the auto-processing and controls the juxtacrine signaling.

Sequence similarities

Belongs to the hedgehog family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    396
  • Mass (Da)
    44,087
  • Last updated
    1996-11-01 v1
  • Checksum
    774A3EC2268A5EE9
MPAVRIVILAICCGLLLVPVRCCGPGRGPVGRRRYMRKLVPLHYKQFVPNVPEKTLGASGKSEGKIHRGSERFIELVPNYNPDIIFKDEEKTGADRLMTERCKDRVNALAISVMNMWPGVKLRVTEGWDEDGHHAHDSLHYEGRALDITTSDRDRNKYGMLARLAVEAGFDWVYYESKAHIHVSVKADNSLGVRSGGCFPGTAMVMMGTGERKPLSELKIGDTVYTTDETGQLITSVVLLFLHRNPYKTATFVLIEAEGHPSKLLVTPNHLLFIQSSSSAGFLPTFAYRVQIGDLVQIYVNGTQVQSSKVVRVSLEEQTGVYAPMTEHGTLLVDGVLTSCYATVESHTLAHVSLAPLRLFQGIASMLPDLDMSDGVHWYCHILYVLAKYVLWWDMP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U26349
EMBL· GenBank· DDBJ
AAA85163.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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