Q8ZRM2 · GLO2_SALTY

Function

function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 metal ions per subunit; upon overexpression in E.coli the enzyme purifies with various amounts of iron, zinc and manganese. The endogenous metal is unknown.

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site53a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site55a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site57a divalent metal cation 2 (UniProtKB | ChEBI)
Binding site58a divalent metal cation 2 (UniProtKB | ChEBI)
Binding site110a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site127a divalent metal cation 1 (UniProtKB | ChEBI)
Binding site127a divalent metal cation 2 (UniProtKB | ChEBI)
Binding site165a divalent metal cation 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionhydroxyacylglutathione hydrolase activity
Molecular Functionmetal ion binding
Biological Processmethylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Hydroxyacylglutathione hydrolase
  • EC number
  • Alternative names
    • Glyoxalase II (Glx II)

Gene names

    • Name
      gloB
    • Ordered locus names
      STM0261

Organism names

Accessions

  • Primary accession
    Q8ZRM2

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003097041-251Hydroxyacylglutathione hydrolase

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    251
  • Mass (Da)
    28,633
  • Last updated
    2002-03-01 v1
  • Checksum
    5A8194A0C921C0E9
MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSRPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEARFAWLRSKKDTF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE006468
EMBL· GenBank· DDBJ
AAL19218.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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