Q8ZRM2 · GLO2_SALTY
- ProteinHydroxyacylglutathione hydrolase
- GenegloB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids251 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Catalytic activity
- an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H+
Cofactor
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 metal ions per subunit; upon overexpression in E.coli the enzyme purifies with various amounts of iron, zinc and manganese. The endogenous metal is unknown.
Pathway
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 53 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 55 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 57 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 58 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 110 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 127 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 127 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 165 | a divalent metal cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydroxyacylglutathione hydrolase activity | |
Molecular Function | metal ion binding | |
Biological Process | methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHydroxyacylglutathione hydrolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionQ8ZRM2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000309704 | 1-251 | Hydroxyacylglutathione hydrolase | |||
Sequence: MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVGGVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIRVLGEKFTLFATPGHTLGHVCYFSRPYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANIKFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLRTEDIDLINEINKETILQQPEARFAWLRSKKDTF |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length251
- Mass (Da)28,633
- Last updated2002-03-01 v1
- Checksum5A8194A0C921C0E9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE006468 EMBL· GenBank· DDBJ | AAL19218.1 EMBL· GenBank· DDBJ | Genomic DNA |