Q8ZL64 · SADA_SALTY

Function

function

Involved in cell aggregation, biofilm formation, and adhesion to human intestinal epithelial cells.

Miscellaneous

Immunization of mice with folded, full-length, purified SadA elicits an IgG response which provides limited protection against bacterial challenge.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell outer membrane
Cellular Componentcell surface
Biological Processprotein transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Autotransporter adhesin SadA
  • Alternative names
    • Salmonella adhesin A
    • Type 5 secretion system autotransporter SadA

Gene names

    • Name
      sadA
    • Ordered locus names
      STM3691

Organism names

Accessions

  • Primary accession
    Q8ZL64

Proteomes

Subcellular Location

Cell surface
Cell outer membrane
Note: The C-terminal translocator domain is localized in the outer membrane and the passenger domain is at the cell surface (By similarity).
Proper surface expression requires SadB (PubMed:24369174).

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1407-1417Beta stranded
Transmembrane1421-1431Beta stranded
Transmembrane1440-1446Beta stranded
Transmembrane1450-1461Beta stranded

Keywords

Phenotypes & Variants

Disruption phenotype

In vitro, loss of sadA does not affect cell aggregation, biofilm formation and adhesion to epithelial cells. Disruption does not affect the course of infection.

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-54
ChainPRO_000043773955-1461Autotransporter adhesin SadA

Proteomic databases

Interaction

Subunit

Homotrimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region55-1372Surface exposed passenger domain
Region1373-1461Translocator domain

Domain

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface (By similarity).
The surface exposed region contains four YadA-like head domains and extended stalk regions, multiply segmented by FGG, HANS, DALL and neck motifs (PubMed:23213248).

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    1,461
  • Mass (Da)
    147,838
  • Last updated
    2002-03-01 v1
  • Checksum
    91C59A87E7282254
MNRIFKVLWNAATGTFVVTSETAKSRGKKNGRRKLAVSALIGLSSIMVSADALANAGNDTGDGVTPTGTQTGGKGWIAIGTDATANTYTNVDGASAAMGYKASAMGKWSTAIGSYSQSTGDSSLALGVKSVSAGDRAIAMGASSSASGSYSMAMGVYANSSGAKSVALGYKSVASGATSSALGYQATASGDDSAAFGNGAKAIGTNSVALGSGSVAQEDNSVAVGNSTTQRQITYVAKGDINSTSTDAVTGAQIYSLSQSVADRLGGGASVNSDGTVNAPLYEVGTGIYNNVGSALSALNTSITNTEASVAGLAEDALLWDESISAFSASHTGNASKITNLAAGTLAADSTDAVNGSQLFDTNEKVDKNTADIATNTGSINQNTADITANTDSINQNTTDIAANTTSINQNTTDIATNTTNINSLSDSVTTLTDDALLWDAASGAFSAKHNGSDSKITNLAAGTLAADSTDAVNGSQLFDTNEKVDQNTADITTNTNSINQNTTDIATNTTNINNLSDSITTLTDDALLWDAASGAFSANHNGSASKITNLAAGTLAADSTDAVNGSQLFATNENVSQNTADITTNTNSINQNTTDIATNTTSINNLSDSITTLTDDALLWDAASGTFSASRSGSASKITNLAAGTLAADSTDAVNGSQLYETNQKVDQNTSAIADINTSITNLSSDNLSWNETTSSFSASHGSSTTNKITNVAAGELSEESTDAVNGSQLFETNEKVDQNTTDIAANTTNITQNSTAIENLNTSVSDINTSITGLTDNALLWDEDTGAFSANHGGSTSKITNVAAGALSEDSTDAVNGSQLYETNQKVDQNTSAIADINTSITNLGTDALSWDDEEGAFSASHGTSGTNKITNVAAGEIASDSTDAVNGSQLYETNMLISQYNESISQLAGDTSETYITENGTGVKYIRTNDNGLEGQDAYATGNGATAVGYDAVASGAGSLALGQNSSSSIEGSIALGSGSTSNRAITTGIRETSATSDGVVIGYNTTDRELLGALSLGTDGESYRQITNVADGSEAQDAVTVRQLQNAIGAVTTTPTKYYHANSTEEDSLAVGTDSLAMGAKTIVNADAGIGIGLNTLVMADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTDYTAYNMDTPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSRNTQSITNLNTQVSNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGADSVAIGSGSIAAAENSVALGTNSVADEANTVSVGSSTQQRRITNVAAGVNNTDAVNVAQLKASEAGSVRYETNADGSVNYSVLNLGDGSGGTTRIGNVSAAVNDTDAVNYAQLKRSVEEANTYTDQKMGEMNSKIKGVENKMSGGIASAMAMAGLPQAYAPGANMTSIAGGTFNGESAVAIGVSMVSESGGWVYKLQGTSNSQGDYSAAIGAGFQW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE006468
EMBL· GenBank· DDBJ
AAL22550.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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