Q8ZCP4 · PDXJ_YERPE
- ProteinPyridoxine 5'-phosphate synthase
- GenepdxJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids243 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.
Catalytic activity
- 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = H+ + 2 H2O + phosphate + pyridoxine 5'-phosphate
Pathway
Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 5/5.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9 | 3-amino-2-oxopropyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 11-12 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: DH | ||||||
Binding site | 20 | 3-amino-2-oxopropyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 45 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 47 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 52 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 72 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 102 | 1-deoxy-D-xylulose 5-phosphate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Site | 153 | Transition state stabilizer | ||||
Sequence: E | ||||||
Active site | 193 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 194 | 3-amino-2-oxopropyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 215-216 | 3-amino-2-oxopropyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: GH |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | pyridoxine 5'-phosphate synthase activity | |
Biological Process | pyridoxine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyridoxine 5'-phosphate synthase
- EC number
- Short namesPNP synthase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia
Accessions
- Primary accessionQ8ZCP4
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000190144 | 1-243 | Pyridoxine 5'-phosphate synthase | |||
Sequence: MADLLLGVNIDHIATLRNARGTIYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMVDIACDIKPHFCCLVPEKRQEVTTEGGLDVAGQVDKMTLAVGRLADVGILVSLFIDADFRQIDAAVAAGAPYIEIHTGAYADASTVLERQAELMRIAKAATYAAGKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMTGLAAAVTDMKVLMREARR |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length243
- Mass (Da)26,297
- Last updated2002-03-05 v1
- Checksum0C369688908E0758
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL590842 EMBL· GenBank· DDBJ | CAL21538.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE009952 EMBL· GenBank· DDBJ | AAM84874.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE017042 EMBL· GenBank· DDBJ | AAS62723.1 EMBL· GenBank· DDBJ | Genomic DNA |