Q8ZC33 · TGT_YERPE

  • Protein
    Queuine tRNA-ribosyltransferase
  • Gene
    tgt
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

tRNA modification; tRNA-queuosine biosynthesis.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site89Proton acceptor
Binding site89-93substrate
Binding site143substrate
Binding site187substrate
Binding site214substrate
Active site264Nucleophile
Binding site302Zn2+ (UniProtKB | ChEBI)
Binding site304Zn2+ (UniProtKB | ChEBI)
Binding site307Zn2+ (UniProtKB | ChEBI)
Binding site333Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Molecular Functionmetal ion binding
Molecular FunctiontRNA-guanosine(34) queuine transglycosylase activity
Biological Processqueuosine biosynthetic process
Biological ProcesstRNA wobble guanine modification
Biological ProcesstRNA-guanine transglycosylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Queuine tRNA-ribosyltransferase
  • EC number
  • Alternative names
    • Guanine insertion enzyme
    • tRNA-guanine transglycosylase

Gene names

    • Name
      tgt
    • Ordered locus names
      YPO3191, y0991, YP_0740

Organism names

  • Taxonomic identifier
  • Strains
    • CO-92 / Biovar Orientalis
    • KIM10+ / Biovar Mediaevalis
    • 91001 / Biovar Mediaevalis
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia

Accessions

  • Primary accession
    Q8ZC33
  • Secondary accessions
    • Q0WC92

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001355611-374Queuine tRNA-ribosyltransferase

Proteomic databases

Interaction

Subunit

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region245-251RNA binding
Region269-273RNA binding; important for wobble base 34 recognition

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    42,633
  • Last updated
    2002-03-01 v1
  • Checksum
    84F52AF00054BF1A
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAARSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDEHCDCYTCRHYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVEDFYGRIGKPVPPLNV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL590842
EMBL· GenBank· DDBJ
CAL21786.1
EMBL· GenBank· DDBJ
Genomic DNA
AE009952
EMBL· GenBank· DDBJ
AAM84572.1
EMBL· GenBank· DDBJ
Genomic DNA
AE017042
EMBL· GenBank· DDBJ
AAS61005.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp