Q8ZAB3 · ILVD_YERPE

  • Protein
    Dihydroxy-acid dehydratase
  • Gene
    ilvD
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site81Mg2+ (UniProtKB | ChEBI)
Binding site122[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site123Mg2+ (UniProtKB | ChEBI)
Binding site124Mg2+ (UniProtKB | ChEBI); via carbamate group
Binding site195[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site491Mg2+ (UniProtKB | ChEBI)
Active site517Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functiondihydroxy-acid dehydratase activity
Molecular Functionhydro-lyase activity
Molecular Functionmagnesium ion binding
Biological Processisoleucine biosynthetic process
Biological Processvaline biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dihydroxy-acid dehydratase
  • EC number
  • Short names
    DAD

Gene names

    • Name
      ilvD
    • Ordered locus names
      YPO3897, y0338, YP_3150

Organism names

  • Taxonomic identifier
  • Strains
    • CO-92 / Biovar Orientalis
    • KIM10+ / Biovar Mediaevalis
    • 91001 / Biovar Mediaevalis
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia

Accessions

  • Primary accession
    Q8ZAB3
  • Secondary accessions
    • Q0WAB5

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001035351-616Dihydroxy-acid dehydratase
Modified residue124N6-carboxylysine

Proteomic databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the IlvD/Edd family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    616
  • Mass (Da)
    65,499
  • Last updated
    2002-03-01 v1
  • Checksum
    07C577F6A71DE77F
MPKYRSHTTTHGRNMAGARALWRATGMTDDDFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIVASGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDKIIKLDLIDAMIQGANPNVSDEESAQIERSACPTCGSCSGMFTANSMNCLNEALGLALPGNGSLLATHADRKQLFLDAGKHIVALTKRYYEQDDVSALPRNIANKAAFENAMILDIAMGGSTNTVLHLLAAAQEGEIDFSMTDIDHLSRKVPHLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVSNVLGLNLTQTLEAYDVMLTQDEGVKQMYAAGPAGIRTTKAFSQDCRYPSLDTDREEGCIRTREHAYSQDGGLAVLYGNIAADGCIVKTAGVDKDSLTFRGPAKVFESQDEAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTTYLKSMGLGKSCALLTDGRFSGGTSGLSIGHVSPEAASGGLIGLVQDGDFINIDIPNRGIVLDVSEAELAARRETEEAHGDAAWSPKGRERQVSYALRAYAMLATSADKGAVRDKSKLGG

Sequence caution

The sequence AAM83929.1 differs from that shown. Reason: Erroneous initiation
The sequence AAS63320.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL590842
EMBL· GenBank· DDBJ
CAL22483.1
EMBL· GenBank· DDBJ
Genomic DNA
AE009952
EMBL· GenBank· DDBJ
AAM83929.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AE017042
EMBL· GenBank· DDBJ
AAS63320.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

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