Q8Y8Q5 · LMCA1_LISMO
- ProteinCalcium-transporting ATPase lmo0841
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids880 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of ATP coupled with the transport of calcium. The transport is electrogenic with a probable ATP:Ca2+:H+ stoichiometry of 1:1:1. May have an important role in survival of the bacterium when stressed by a combination of a high calcium concentration and alkaline pH.
Catalytic activity
- ATP + Ca2+(in) + H2O = ADP + Ca2+(out) + H+ + phosphate
CHEBI:30616 + Ca2+ (in)CHEBI:29108+ CHEBI:15377 = CHEBI:456216 + Ca2+ (out)CHEBI:29108+ CHEBI:15378 + CHEBI:43474
Activity regulation
Phosphorylation is inhibited by EGTA and vanadate. ATPase activity is stimulated by Sr2+. Inhibited by very high concentrations of cyclopiazonic acid (CPA).
pH Dependence
Optimum pH is 9.0.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 287 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 288 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 290 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 292 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 334 | 4-aspartylphosphate intermediate | ||||
Sequence: D | ||||||
Binding site | 716 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 720 | Ca2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | metal ion binding | |
Molecular Function | P-type calcium transporter activity | |
Molecular Function | P-type ion transporter activity | |
Biological Process | monoatomic ion transmembrane transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-transporting ATPase lmo0841
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Listeriaceae > Listeria
Accessions
- Primary accessionQ8Y8Q5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 47-67 | Helical | ||||
Sequence: LWKLFLETFKDPMVIVLVIAA | ||||||
Transmembrane | 68-88 | Helical | ||||
Sequence: LVQLVLGEVVESLIIFLVLIV | ||||||
Transmembrane | 243-263 | Helical | ||||
Sequence: LGLGILALCVLIFAVEAGRVL | ||||||
Transmembrane | 271-291 | Helical | ||||
Sequence: MATAILNAFMFAVAVAVAAIP | ||||||
Transmembrane | 681-701 | Helical | ||||
Sequence: IAYLFAGNLGAIIAILFALVL | ||||||
Transmembrane | 707-727 | Helical | ||||
Sequence: FTALQLLFINLVNDSLPAIAL | ||||||
Transmembrane | 756-776 | Helical | ||||
Sequence: AVISRGVLIGIAVIISQYIGM | ||||||
Transmembrane | 819-839 | Helical | ||||
Sequence: YVIGAVLLCFVLYGITVLPGA | ||||||
Transmembrane | 854-874 | Helical | ||||
Sequence: WSIAAGLALAAVVMMEIIKVV |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 54 | Increases sensitivity to CPA. CPA-sensitive; when associated with P-295. | ||||
Sequence: T → Q | ||||||
Mutagenesis | 59 | Does not affect sensitivity to CPA. | ||||
Sequence: M → L | ||||||
Mutagenesis | 240 | Does not affect sensitivity to CPA. | ||||
Sequence: S → G | ||||||
Mutagenesis | 295 | Strongly increases sensitivity to CPA. CPA-sensitive; when associated with Q-54. | ||||
Sequence: S → P | ||||||
Mutagenesis | 691 | Displays optimal activity near pH 8.0. | ||||
Sequence: A → E | ||||||
Mutagenesis | 795 | Displays optimal activity near pH 8.0. | ||||
Sequence: R → E | ||||||
Mutagenesis | 795 | Shows very low activity, but no change in pH-dependence. | ||||
Sequence: R → K | ||||||
Mutagenesis | 795 | Displays optimal activity near pH 8.5. | ||||
Sequence: R → Q |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000403478 | 1-880 | Calcium-transporting ATPase lmo0841 | |||
Sequence: MEIYRKSAAETFTQLEATEKGLTTSEVTKRQEKYGFNELKNKKKDPLWKLFLETFKDPMVIVLVIAALVQLVLGEVVESLIIFLVLIVNSIISVVQTRKAESSLDALREMSAPVAKVIRDGSKQSIHARELVPGDVVILDAGDFVPADGRLFESGSLKIDEGMLTGESEAVEKYIDTIPDEVGLGDRVNMVFSGSLVVYGRGMFVVTGTASETEIGKIAGLLETAEAKQTPLQRKLESFSKKLGLGILALCVLIFAVEAGRVLLGDNSADMATAILNAFMFAVAVAVAAIPEALSSIVTIVLAVGTNKMAKQHAIIRKLPAVETLGSTSVICTDKTGTLTQNKMTVVDYYLPDGTKENFPESPENWSEGERRLIHIAVLCNDSNINSEGKELGDPTEVALIAFSNKNNQDYNEIREKFIREGEIPFDSDRKLMSTLHTFNENKAMLTKGGPDVMFARCSYVFLDGEEKPMTEEILAKLKETNEEFSNQALRVLAYGYKRMPADTTELKLEDEQDIVLVGLTAMIDPPREAVYASIEESKKAGIRTVMITGDHKTTAQAIGRDIGLMDADDIALTGQELDAMPEEELDKKLEHIAVYARVSPENKIRIVKAWQKKGKITAMTGDGVNDAPALKQADIGVAMGSGTDVAKDSAAMILTDDNFVSIVDAVGVGRTVFDNIKKSIAYLFAGNLGAIIAILFALVLDWINPFTALQLLFINLVNDSLPAIALGMEKAEPDVMKRKPRDINEGIFAGGTMRAVISRGVLIGIAVIISQYIGMQISPEMSVAMAFTTLILARTLQTFAARSNVQTAFGAGFFSNKYVIGAVLLCFVLYGITVLPGAREIFSIPASFGLHEWSIAAGLALAAVVMMEIIKVVQNKFFK |
Keywords
- PTM
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length880
- Mass (Da)95,658
- Last updated2002-03-01 v1
- ChecksumED088BDD461D29B1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL591976 EMBL· GenBank· DDBJ | CAC98919.1 EMBL· GenBank· DDBJ | Genomic DNA |