Q8Y3L4 · INLJ_LISMO
- ProteinInternalin J
- GeneinlJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids851 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in several steps of L.monocytogenes infection by both intravenous and oral infection (PubMed:16177371).
Probably acts as an adhesion; upon ectopic expression in L.innocula bacteria adhere better to human cell lines (PubMed:18227172).
Probably acts as an adhesion; upon ectopic expression in L.innocula bacteria adhere better to human cell lines (PubMed:18227172).
Activity regulation
Despite being transcribed during bacterial growth in culture the protein is only detected in infected mice.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | signaling adaptor activity | |
Biological Process | cell adhesion |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInternalin J
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Listeriaceae > Listeria
Accessions
- Primary accessionQ8Y3L4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Secreted, cell wall ; Peptidoglycan-anchor
Note: No protein is expressed when grown in liquid culture; protein is detected 72 hours post-inoculation in intravenously infected mice (PubMed:18227172).
Upon expression under control of a heterologous promoter during exponential growth is detected on the cell surface as a series of dots in a helical pattern, it is excluded from the septum; at very high expression levels it accumulates at cell poles (PubMed:21725001).
The helical pattern of InlA does not overlap with that of InlH, InlJ or Hbp2 (SvpA) (PubMed:21725001).
In stationary phase remains outside of the cell pole and septum (PubMed:21725001).
Upon expression under control of a heterologous promoter during exponential growth is detected on the cell surface as a series of dots in a helical pattern, it is excluded from the septum; at very high expression levels it accumulates at cell poles (PubMed:21725001).
The helical pattern of InlA does not overlap with that of InlH, InlJ or Hbp2 (SvpA) (PubMed:21725001).
In stationary phase remains outside of the cell pole and septum (PubMed:21725001).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible effect on colonization of host tissue culture cells; decreased organ colonization in mice.
PTM/Processing
Features
Showing features for signal, chain, modified residue, propeptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-25 | |||||
Sequence: MKTTKIVIASLVSLTMVSNPLLTFA | ||||||
Chain | PRO_0000252679 | 26-824 | Internalin J | |||
Sequence: ATNDVIDNTTEITTDKETSSTQPTIKNTLKAGQTQSFNDWFPDDNFASEVAAAFEMQATDTISEEQLATLTSLDCHNSSITDMTGIEKLTGLTKLICTSNNITTLDLSQNTNLTYLACDSNKLTNLDVTPLTKLTYLNCDTNKLTKLDVSQNPLLTYLNCARNTLTEIDVSHNTQLTELDCHLNKKITKLDVTPQTQLTTLDCSFNKITELDVSQNKLLNRLNCDTNNITKLDLNQNIQLTFLDCSSNKLTEIDVTPLTQLTYFDCSVNPLTELDVSTLSKLTTLHCIQTDLLEIDLTHNTQLIYFQAEGCRKIKELDVTHNTQLYLLDCQAAGITELDLSQNPKLVYLYLNNTELTELDVSHNTKLKSLSCVNAHIQDFSSVGKIPALNNNFEAEGQTITMPKETLTNNSLTIAVSPDLLDQFGNPMNIEPGDGGVYDQATNTITWENLSTDNPAVTYTFTSENGAIVGTVTTPFEAPQPIKGEDVTVHYLDDKGEKLADDEVLSGNLDDPYTSSAKDIPDYTLTTTPDNATGTFTTTSQSVTYVYTKNIVAAEPVTVNYVDDTGKTLSPSEILNGNVGDTYNATAKQIDGYTLSAEPTNATGQFTSSAQTVNYIYTKNPAPEKGVVEIHYVDEDNKQLNSTTEISGTIGDNYTTEPKTIEGYTLTTTPGNATGTFTTGSQTVTYVYTKNIEAAEPITVNYVDANGKTLAPSETLNGNVGDTYKATAKQIDGYTLSAEPTNATGQFTSSAQTVNYIYTKNTNTDQPLPTKKPTNTTPTKPSNLKTTEVKKASDTLPKT | ||||||
Modified residue | 824 | Pentaglycyl murein peptidoglycan amidated threonine | ||||
Sequence: T | ||||||
Propeptide | PRO_0000252680 | 825-851 | Removed by sortase A | |||
Sequence: GDSAPWKSALLGVFLSSTALVIWKKKK |
Keywords
- PTM
Proteomic databases
Expression
Induction
Expressed in late exponential phase.
Interaction
Subunit
Nearly full-length mature protein and an internal LRR-containing fragment interact in vitro with human intestinal mucin-2 (MUC2) but not with mucin-1. LRR fragment binding is slightly better at pH 5.5, (the pH of the intestine) than at pH 7.4.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat, domain, compositional bias, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 94-115 | LRR 1 | ||||
Sequence: TLTSLDCHNSSITDMTGIEKLT | ||||||
Repeat | 116-136 | LRR 2 | ||||
Sequence: GLTKLICTSNNITTLDLSQNT | ||||||
Repeat | 137-157 | LRR 3 | ||||
Sequence: NLTYLACDSNKLTNLDVTPLT | ||||||
Repeat | 158-179 | LRR 4 | ||||
Sequence: KLTYLNCDTNKLTKLDVSQNPL | ||||||
Repeat | 180-200 | LRR 5 | ||||
Sequence: LTYLNCARNTLTEIDVSHNTQ | ||||||
Repeat | 201-221 | LRR 6 | ||||
Sequence: LTELDCHLNKKITKLDVTPQT | ||||||
Repeat | 222-243 | LRR 7 | ||||
Sequence: QLTTLDCSFNKITELDVSQNKL | ||||||
Repeat | 244-263 | LRR 8 | ||||
Sequence: LNRLNCDTNNITKLDLNQNI | ||||||
Repeat | 264-284 | LRR 9 | ||||
Sequence: QLTFLDCSSNKLTEIDVTPLT | ||||||
Repeat | 285-306 | LRR 10 | ||||
Sequence: QLTYFDCSVNPLTELDVSTLSK | ||||||
Repeat | 316-325 | LRR 11 | ||||
Sequence: DLLEIDLTHN | ||||||
Repeat | 338-357 | LRR 12 | ||||
Sequence: KIKELDVTHNTQLYLLDCQA | ||||||
Repeat | 359-368 | LRR 13 | ||||
Sequence: GITELDLSQN | ||||||
Repeat | 380-402 | LRR 14 | ||||
Sequence: ELTELDVSHNTKLKSLSCVNAHI | ||||||
Domain | 506-568 | MucBP 1 | ||||
Sequence: PIKGEDVTVHYLDDKGEKLADDEVLSGNLDDPYTSSAKDIPDYTLTTTPDNATGTFTTTSQSV | ||||||
Domain | 576-638 | MucBP 2 | ||||
Sequence: IVAAEPVTVNYVDDTGKTLSPSEILNGNVGDTYNATAKQIDGYTLSAEPTNATGQFTSSAQTV | ||||||
Domain | 647-709 | MucBP 3 | ||||
Sequence: APEKGVVEIHYVDEDNKQLNSTTEISGTIGDNYTTEPKTIEGYTLTTTPGNATGTFTTGSQTV | ||||||
Domain | 717-779 | MucBP 4 | ||||
Sequence: IEAAEPITVNYVDANGKTLAPSETLNGNVGDTYKATAKQIDGYTLSAEPTNATGQFTSSAQTV | ||||||
Compositional bias | 786-815 | Polar residues | ||||
Sequence: NTNTDQPLPTKKPTNTTPTKPSNLKTTEVK | ||||||
Region | 786-825 | Disordered | ||||
Sequence: NTNTDQPLPTKKPTNTTPTKPSNLKTTEVKKASDTLPKTG | ||||||
Motif | 821-825 | LPXTG sorting signal | ||||
Sequence: LPKTG |
Domain
The LRR domain (34-508) binds more efficiently in vitro to human intestinal mucin-2 (MUC2) than does a nearly whole protein (26-792) suggesting the MucBP domains do not function in mucin-binding in Listeria.
Sequence similarities
Belongs to the internalin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length851
- Mass (Da)92,803
- Last updated2002-03-01 v1
- Checksum7510519A85D53A55
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 786-815 | Polar residues | ||||
Sequence: NTNTDQPLPTKKPTNTTPTKPSNLKTTEVK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL591984 EMBL· GenBank· DDBJ | CAD01034.1 EMBL· GenBank· DDBJ | Genomic DNA |