Q8XQC5 · SPEE2_RALN1
- ProteinPolyamine aminopropyltransferase 2
- GenespeE2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids510 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.
Catalytic activity
- putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H+ + S-methyl-5'-thioadenosine + spermidine
Pathway
Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 244 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 274 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 298 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 318 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 352-353 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Active site | 370 | Proton acceptor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | spermidine synthase activity | |
Biological Process | spermidine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyamine aminopropyltransferase 2
- Alternative names
Gene names
Encoded on
- Plasmid megaplasmid Rsp
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Ralstonia > Ralstonia solanacearum species complex
Accessions
- Primary accessionQ8XQC5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 6-26 | Helical | ||||
Sequence: ALLVLAVFVVASCGLAYELIA | ||||||
Transmembrane | 38-58 | Helical | ||||
Sequence: ILQFSSIIGAYLFAMGIGSWV | ||||||
Transmembrane | 74-94 | Helical | ||||
Sequence: LELLVGLFGGVSAAALFLLFA | ||||||
Transmembrane | 102-122 | Helical | ||||
Sequence: LVLYALVTVIGVLVGMEIPLV | ||||||
Transmembrane | 140-160 | Helical | ||||
Sequence: VLTFDYLGALAVSLLFPLVLA | ||||||
Transmembrane | 165-185 | Helical | ||||
Sequence: LVRTGFLFGLCNTAIAVWTLW |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000156500 | 1-510 | Polyamine aminopropyltransferase 2 | |||
Sequence: MNRGNALLVLAVFVVASCGLAYELIAGALASYLLGDSILQFSSIIGAYLFAMGIGSWVSRYVADDALLARFVDLELLVGLFGGVSAAALFLLFALESAPFRLVLYALVTVIGVLVGMEIPLVMRMLHRRQAKFSDLVSRVLTFDYLGALAVSLLFPLVLAPRLGLVRTGFLFGLCNTAIAVWTLWHFRAELGLSARLRGAMAWRAGMVGAALLAGFAASDRLTHWSERALFGDEIIHAISSPYQRLVVTRWKDDLRLYINGNLQFSSRDEYRYHEALVLPALESVRGARRVLVLGGGDGLALRQILKYPQVEHVTLVDLDPRMTSLFSHAEALVALNQHAFSDPRVTVVNADAGQWLQTAADMFDVAIVDFPDPSNFSIGKLYSVPFYRLLSRHVADTGLVVIQATSPYFAPRSYWCVDATLKEAGYRTWPYHALVPSFGEWGFILAAPGRADFRPPTTYRVPTRFLDADTTHQMFSFAPDMPRPQVEPNRLNNQSLVRYFEEDWHGVLR |
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 205-449 | PABS | ||||
Sequence: AGMVGAALLAGFAASDRLTHWSERALFGDEIIHAISSPYQRLVVTRWKDDLRLYINGNLQFSSRDEYRYHEALVLPALESVRGARRVLVLGGGDGLALRQILKYPQVEHVTLVDLDPRMTSLFSHAEALVALNQHAFSDPRVTVVNADAGQWLQTAADMFDVAIVDFPDPSNFSIGKLYSVPFYRLLSRHVADTGLVVIQATSPYFAPRSYWCVDATLKEAGYRTWPYHALVPSFGEWGFILAAP | ||||||
Region | 207-456 | Spermidine synthase | ||||
Sequence: MVGAALLAGFAASDRLTHWSERALFGDEIIHAISSPYQRLVVTRWKDDLRLYINGNLQFSSRDEYRYHEALVLPALESVRGARRVLVLGGGDGLALRQILKYPQVEHVTLVDLDPRMTSLFSHAEALVALNQHAFSDPRVTVVNADAGQWLQTAADMFDVAIVDFPDPSNFSIGKLYSVPFYRLLSRHVADTGLVVIQATSPYFAPRSYWCVDATLKEAGYRTWPYHALVPSFGEWGFILAAPGRADFRP |
Sequence similarities
Belongs to the spermidine/spermine synthase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length510
- Mass (Da)56,611
- Last updated2002-03-01 v1
- Checksum1814AE8BDA2A56AB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL646053 EMBL· GenBank· DDBJ | CAD18488.1 EMBL· GenBank· DDBJ | Genomic DNA |