Q8XKU1 · TPIS_CLOPE
- ProteinTriosephosphate isomerase
- GenetpiA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids248 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).
Catalytic activity
- D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.72 mM | G3P |
kcat is 6348 sec-1 for isomerase activity.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9-11 | substrate | ||||
Sequence: NWK | ||||||
Active site | 94 | Electrophile | ||||
Sequence: H | ||||||
Active site | 166 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 172 | substrate | ||||
Sequence: G | ||||||
Binding site | 212 | substrate | ||||
Sequence: S | ||||||
Binding site | 233-234 | substrate | ||||
Sequence: GG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | triose-phosphate isomerase activity | |
Biological Process | gluconeogenesis | |
Biological Process | glycolytic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTriosephosphate isomerase
- EC number
- Short namesTIM ; TPI
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionQ8XKU1
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000090211 | 1-248 | Triosephosphate isomerase | |||
Sequence: MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKVGAQNMHFEEKGAFTGEIAPRMLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNLTPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEKVVIAYEPIWAIGTGKTATSDQANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAADFAQIVNY |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length248
- Mass (Da)27,058
- Last updated2002-03-27 v1
- Checksum8503792DED4B573A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000016 EMBL· GenBank· DDBJ | BAB81008.1 EMBL· GenBank· DDBJ | Genomic DNA |