Q8X8T3 · HISX_ECO57
- ProteinHistidinol dehydrogenase
- GenehisD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids434 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.
Catalytic activity
- H2O + L-histidinol + 2 NAD+ = 3 H+ + L-histidine + 2 NADH
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 130 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 188 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 211 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 237 | substrate | ||||
Sequence: S | ||||||
Binding site | 259 | substrate | ||||
Sequence: Q | ||||||
Binding site | 259 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 262 | substrate | ||||
Sequence: H | ||||||
Binding site | 262 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 326 | Proton acceptor | ||||
Sequence: E | ||||||
Active site | 327 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 327 | substrate | ||||
Sequence: H | ||||||
Binding site | 360 | substrate | ||||
Sequence: D | ||||||
Binding site | 360 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 414 | substrate | ||||
Sequence: E | ||||||
Binding site | 419 | substrate | ||||
Sequence: H | ||||||
Binding site | 419 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | histidinol dehydrogenase activity | |
Molecular Function | NAD binding | |
Molecular Function | zinc ion binding | |
Biological Process | L-histidine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistidinol dehydrogenase
- EC number
- Short namesHDH
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ8X8T3
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000135771 | 2-434 | Histidinol dehydrogenase | |||
Sequence: SFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVKARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQKLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLCSPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAKRQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDADMAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRNARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTVQELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length434
- Mass (Da)46,074
- Last updated2007-01-23 v3
- ChecksumA280AE06E39689C8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB008676 EMBL· GenBank· DDBJ | BAA77745.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE005174 EMBL· GenBank· DDBJ | AAG57079.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000007 EMBL· GenBank· DDBJ | BAB36244.1 EMBL· GenBank· DDBJ | Genomic DNA |