Q8W104 · FBL17_ARATH
- ProteinF-box/LRR-repeat protein 17
- GeneFBL17
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids593 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Essential protein for male fertility. Component of the SCF(ASK-cullin-F-box) E3 ubiquitin ligase complex SCF(FBL17), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Enables the switch in cell cycle control leading to male germ cell lineage formation from microspores after meiosis. Targets CDKA-1 inhibitors the degradation specifically in male germ cells (e.g. KRP6 and KRP7) and thus enables CDKA-1 activation and germ cell S-phase progression. Promotes twin sperm cell production and double fertilization.
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | male germ cell nucleus | |
Cellular Component | nucleus | |
Cellular Component | SCF ubiquitin ligase complex | |
Cellular Component | site of double-strand break | |
Biological Process | DNA damage response | |
Biological Process | embryo development ending in seed dormancy | |
Biological Process | generative cell mitosis | |
Biological Process | pollen development | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of cell division | |
Biological Process | SCF-dependent proteasomal ubiquitin-dependent protein catabolic process | |
Biological Process | seed development |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameF-box/LRR-repeat protein 17
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8W104
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Confined to the male germ cell nucleus of early to mid bicellular pollen.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Stabilization of KRP6 and inhibition of germ cell cycle progression leading to male sterility.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 29 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000272257 | 1-593 | F-box/LRR-repeat protein 17 | |||
Sequence: MQPQPHISPNTATAAISAALESQRSRKNRGSYNCGRCGQPKKGHVCLLTAPPDIPTTPIASEPVSCISAAASSSRSTVLSLTAAPSSRQTFTHLRRALSFDDVDARNSLDESDLDAASMDLDLQLDTDIVQPGRFHAVGLWEVLKRLPPSSLLMAARVCKGWRETSRKMWKAAEELRIRVPERAQIGYIGSLLQKCPRLIRLSLKIESDFDATTLACIAFSCPNLEVLEITTSGAAVNRISGDELSRFVANKRGLTSLKMEGCSNLGGFSLSSSSLSTLWLSDLHSLSKMIFNCPNLTEISLEFSRQEDDSTDLVTMVDGLGRTCTRLQNIHIASLKLSHTVVLSLTAVNFRYLRMLSLVLGINITDASVAAISSGYKNLELLDLSGSSITDTGLGMICDVLPDTLSKLLVALCPNITSSGIQFATAQLPLLELMDCGMTVSDPNSDNPTFVENPSPHKTPGYNQKMFIKHKRLKKLSLWGCSSLDALFLNCPELMDLNLNLCSNLHPESLVLQCPKLQLVYASGCQGLLTGAIRKQVSENFSAGENHMPRKRLADASKRIQALPSLYQETREDGIYAGKRRKLEKEMCTIIH |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in developing pollen.
Developmental stage
Expressed transiently in the male germ line after asymmetric division. Present in maturing pollen with highest levels in bicellular pollen.
Gene expression databases
Interaction
Subunit
Part of a SCF (ASK-cullin-F-box) protein ligase complex. Interacts with SKP1A/ASK1, KRP4, KRP6 and KRP7.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8W104 | KRP6 Q0WNX9 | 3 | EBI-2026732, EBI-1253171 | |
BINARY | Q8W104 | KRP7 Q94CL9 | 4 | EBI-2026732, EBI-1773344 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 120-177 | F-box | ||||
Sequence: DLDLQLDTDIVQPGRFHAVGLWEVLKRLPPSSLLMAARVCKGWRETSRKMWKAAEELR | ||||||
Repeat | 178-206 | LRR 1 | ||||
Sequence: IRVPERAQIGYIGSLLQKCPRLIRLSLKI | ||||||
Repeat | 207-232 | LRR 2 | ||||
Sequence: ESDFDATTLACIAFSCPNLEVLEITT | ||||||
Repeat | 237-262 | LRR 3 | ||||
Sequence: VNRISGDELSRFVANKRGLTSLKMEG | ||||||
Repeat | 276-304 | LRR 4 | ||||
Sequence: LSTLWLSDLHSLSKMIFNCPNLTEISLEF | ||||||
Repeat | 335-361 | LRR 5 | ||||
Sequence: SLKLSHTVVLSLTAVNFRYLRMLSLVL | ||||||
Repeat | 362-387 | LRR 6 | ||||
Sequence: GINITDASVAAISSGYKNLELLDLSG | ||||||
Repeat | 414-439 | LRR 7 | ||||
Sequence: CPNITSSGIQFATAQLPLLELMDCGM | ||||||
Repeat | 477-502 | LRR 8 | ||||
Sequence: LSLWGCSSLDALFLNCPELMDLNLNL | ||||||
Repeat | 503-525 | LRR 9 | ||||
Sequence: CSNLHPESLVLQCPKLQLVYASG |
Domain
The F-box is necessary for the interaction with ASK proteins.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length593
- Mass (Da)64,769
- Last updated2002-03-01 v1
- ChecksumB7C9668303455BB3
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL138650 EMBL· GenBank· DDBJ | CAB77585.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE79261.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF462827 EMBL· GenBank· DDBJ | AAL58916.1 EMBL· GenBank· DDBJ | mRNA | ||
AY142006 EMBL· GenBank· DDBJ | AAM98270.1 EMBL· GenBank· DDBJ | mRNA |