Q8W033 · AL3I1_ARATH
- ProteinAldehyde dehydrogenase family 3 member I1, chloroplastic
- GeneALDH3I1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids550 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD+ and NADP+, but the coenzyme preference is substrate dependent.
Miscellaneous
Plants overexpressing ALDH3I1 show improved tolerance when exposed to dehydration, salt stress, heavy metals and H2O2.
Catalytic activity
- an aldehyde + H2O + NAD+ = a carboxylate + 2 H+ + NADH
Activity regulation
Thiol-based regulation. Inactivation after dimerization under oxidizing conditions.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
8053 μM | propionaldehyde | |||||
111 μM | hexanal | |||||
24 μM | octanal | |||||
7 μM | nonanal | |||||
1.3 μM | dodecanal | |||||
151 μM | trans-2-hexenal | |||||
5.5 μM | trans-2-nonenal | |||||
21 μM | 4-hydroxynonenal | |||||
71 μM | NAD+ | in the presence of hexanal as co-substrate | ||||
1868 μM | NADP+ | in the presence of hexanal as co-substrate | ||||
53 μM | NAD+ | in the presence of trans-2-nonenal as co-substrate | ||||
87 μM | NADP+ | in the presence of trans-2-nonenal as co-substrate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
10.1 μmol/min/mg | with propionaldehyde as substrate | ||||
17.3 μmol/min/mg | with hexanal as substrate | ||||
16.6 μmol/min/mg | with octanal as substrate | ||||
20 μmol/min/mg | with nonanal as substrate | ||||
18.8 μmol/min/mg | with dodecanal as substrate | ||||
1.5 μmol/min/mg | with trans-2-hexenal as substrate | ||||
1.6 μmol/min/mg | with trans-2-nonenal as substrate | ||||
0.6 μmol/min/mg | with 4-hydroxynonenal as substrate |
pH Dependence
Optimum pH is 9.0.
Features
Showing features for site, binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast envelope | |
Cellular Component | plasma membrane | |
Cellular Component | plastid | |
Molecular Function | 3-chloroallyl aldehyde dehydrogenase activity | |
Molecular Function | aldehyde dehydrogenase (NAD+) activity | |
Molecular Function | aldehyde dehydrogenase (NADP+) activity | |
Molecular Function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity | |
Biological Process | cellular aldehyde metabolic process | |
Biological Process | response to abscisic acid | |
Biological Process | response to water deprivation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAldehyde dehydrogenase family 3 member I1, chloroplastic
- EC number
- Short namesAtALDH3; Ath-ALDH3
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8W033
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 114 | No effect on solubility, but loss of dimerization and 80% loss of activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 142 | No effect on solubility, but decreased activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 263 | No effect on substrate specificity, but decreased affinity for NADP+ and increased affinity for NAD+. | ||||
Sequence: V → I | ||||||
Mutagenesis | 286 | No effect on solubility, but no effect on activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 310 | No effect on solubility, but no effect on activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 316 | No effect on solubility, but loss of activity. | ||||
Sequence: C → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 53 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-59 | Chloroplast | ||||
Sequence: MTKLLEINHIQTLCFAKGFSPARLNVATSPFRISRRGGGGYCSNACIPYRLKFTCYATL | ||||||
Chain | PRO_0000256061 | 60-550 | Aldehyde dehydrogenase family 3 member I1, chloroplastic | |||
Sequence: SAVVKEQASDFSGKEAALLVDELRSNFNSGRTKSYEWRISQLQNIARMIDEKEKCITEALYQDLSKPELEAFLAEISNTKSSCMLAIKELKNWMAPETVKTSVTTFPSSAQIVSEPLGVVLVISAWNFPFLLSVEPVIGAIAAGNAVVLKPSEIAPAASSLLAKLFSEYLDNTTIRVIEGGVPETTALLDQKWDKIFFTGGARVARIIMAAAARNLTPVVLELGGKCPALVDSDVNLQVAARRIIAGKWACNSGQACIGVDYVITTKDFASKLIDALKTELETFFGQNALESKDLSRIVNSFHFKRLESMLKENGVANKIVHGGRITEDKLKISPTILLDVPEASSMMQEEIFGPLLPIITVQKIEDGFQVIRSKPKPLAAYLFTNNKELEKQFVQDVSAGGITINDTVLHVTVKDLPFGGVGESGIGAYHGKFSYETFSHKKGVLYRSFSGDADLRYPPYTPKKKMVLKALLSSNIFAAILAFFGFSKDS | ||||||
Disulfide bond | 114 | Interchain | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By abscisic acid (ABA), dehydration, salt stress in plantlets. Induced by heavy metals and H2O2.
Gene expression databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q8W033-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length550
- Mass (Da)60,173
- Last updated2006-10-31 v2
- Checksum42FC2BC37757A083
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8B7C0 | A0A1P8B7C0_ARATH | ALDH3I1 | 535 | ||
A0A1P8B7C2 | A0A1P8B7C2_ARATH | ALDH3I1 | 408 | ||
F4JKM4 | F4JKM4_ARATH | ALDH3I1 | 390 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 32 | in Ref. 1; CAC84903 | ||||
Sequence: R → L | ||||||
Sequence conflict | 71 | in Ref. 1; CAC84903 | ||||
Sequence: S → R | ||||||
Sequence conflict | 435 | in Ref. 1; CAC84903 | ||||
Sequence: P → S | ||||||
Sequence conflict | 536 | in Ref. 1; CAC84903 | ||||
Sequence: I → M |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ306961 EMBL· GenBank· DDBJ | CAC84903.1 EMBL· GenBank· DDBJ | mRNA | ||
AL035521 EMBL· GenBank· DDBJ | CAB36701.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AL161585 EMBL· GenBank· DDBJ | CAB80141.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002687 EMBL· GenBank· DDBJ | AEE86347.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY054633 EMBL· GenBank· DDBJ | AAK96824.1 EMBL· GenBank· DDBJ | mRNA | ||
AY081532 EMBL· GenBank· DDBJ | AAM10094.1 EMBL· GenBank· DDBJ | mRNA |