Q8W033 · AL3I1_ARATH

Function

function

Involved in oxidative stress tolerance by detoxifying reactive aldehydes derived from lipid peroxidation. Medium- to long-chain saturated aldehydes are preferred substrates, while the short-chain aldehyde propanal is a weak substrate. Can use both NAD+ and NADP+, but the coenzyme preference is substrate dependent.

Miscellaneous

Plants overexpressing ALDH3I1 show improved tolerance when exposed to dehydration, salt stress, heavy metals and H2O2.

Catalytic activity

Activity regulation

Thiol-based regulation. Inactivation after dimerization under oxidizing conditions.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
8053 μMpropionaldehyde
111 μMhexanal
24 μMoctanal
7 μMnonanal
1.3 μMdodecanal
151 μMtrans-2-hexenal
5.5 μMtrans-2-nonenal
21 μM4-hydroxynonenal
71 μMNAD+in the presence of hexanal as co-substrate
1868 μMNADP+in the presence of hexanal as co-substrate
53 μMNAD+in the presence of trans-2-nonenal as co-substrate
87 μMNADP+in the presence of trans-2-nonenal as co-substrate
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
10.1 μmol/min/mgwith propionaldehyde as substrate
17.3 μmol/min/mgwith hexanal as substrate
16.6 μmol/min/mgwith octanal as substrate
20 μmol/min/mgwith nonanal as substrate
18.8 μmol/min/mgwith dodecanal as substrate
1.5 μmol/min/mgwith trans-2-hexenal as substrate
1.6 μmol/min/mgwith trans-2-nonenal as substrate
0.6 μmol/min/mgwith 4-hydroxynonenal as substrate

pH Dependence

Optimum pH is 9.0.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site186Transition state stabilizer
Binding site259-264NAD+ (UniProtKB | ChEBI)
Active site281Proton acceptor
Active site316Nucleophile

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast envelope
Cellular Componentplasma membrane
Cellular Componentplastid
Molecular Function3-chloroallyl aldehyde dehydrogenase activity
Molecular Functionaldehyde dehydrogenase (NAD+) activity
Molecular Functionaldehyde dehydrogenase (NADP+) activity
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity
Biological Processcellular aldehyde metabolic process
Biological Processresponse to abscisic acid
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Aldehyde dehydrogenase family 3 member I1, chloroplastic
  • EC number
  • Short names
    AtALDH3; Ath-ALDH3

Gene names

    • Name
      ALDH3I1
    • Synonyms
      ALDH3
    • ORF names
      F10M10.10
    • Ordered locus names
      At4g34240

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8W033
  • Secondary accessions
    • Q940H4
    • Q9SYY9

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis114No effect on solubility, but loss of dimerization and 80% loss of activity.
Mutagenesis142No effect on solubility, but decreased activity.
Mutagenesis263No effect on substrate specificity, but decreased affinity for NADP+ and increased affinity for NAD+.
Mutagenesis286No effect on solubility, but no effect on activity.
Mutagenesis310No effect on solubility, but no effect on activity.
Mutagenesis316No effect on solubility, but loss of activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 53 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain, disulfide bond.

TypeIDPosition(s)Description
Transit peptide1-59Chloroplast
ChainPRO_000025606160-550Aldehyde dehydrogenase family 3 member I1, chloroplastic
Disulfide bond114Interchain

Keywords

Proteomic databases

PTM databases

Expression

Induction

By abscisic acid (ABA), dehydration, salt stress in plantlets. Induced by heavy metals and H2O2.

Gene expression databases

Interaction

Subunit

Homodimer and homomultimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q8W033-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    550
  • Mass (Da)
    60,173
  • Last updated
    2006-10-31 v2
  • Checksum
    42FC2BC37757A083
MTKLLEINHIQTLCFAKGFSPARLNVATSPFRISRRGGGGYCSNACIPYRLKFTCYATLSAVVKEQASDFSGKEAALLVDELRSNFNSGRTKSYEWRISQLQNIARMIDEKEKCITEALYQDLSKPELEAFLAEISNTKSSCMLAIKELKNWMAPETVKTSVTTFPSSAQIVSEPLGVVLVISAWNFPFLLSVEPVIGAIAAGNAVVLKPSEIAPAASSLLAKLFSEYLDNTTIRVIEGGVPETTALLDQKWDKIFFTGGARVARIIMAAAARNLTPVVLELGGKCPALVDSDVNLQVAARRIIAGKWACNSGQACIGVDYVITTKDFASKLIDALKTELETFFGQNALESKDLSRIVNSFHFKRLESMLKENGVANKIVHGGRITEDKLKISPTILLDVPEASSMMQEEIFGPLLPIITVQKIEDGFQVIRSKPKPLAAYLFTNNKELEKQFVQDVSAGGITINDTVLHVTVKDLPFGGVGESGIGAYHGKFSYETFSHKKGVLYRSFSGDADLRYPPYTPKKKMVLKALLSSNIFAAILAFFGFSKDS

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8B7C0A0A1P8B7C0_ARATHALDH3I1535
A0A1P8B7C2A0A1P8B7C2_ARATHALDH3I1408
F4JKM4F4JKM4_ARATHALDH3I1390

Sequence caution

The sequence CAB36701.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB80141.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict32in Ref. 1; CAC84903
Sequence conflict71in Ref. 1; CAC84903
Sequence conflict435in Ref. 1; CAC84903
Sequence conflict536in Ref. 1; CAC84903

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ306961
EMBL· GenBank· DDBJ
CAC84903.1
EMBL· GenBank· DDBJ
mRNA
AL035521
EMBL· GenBank· DDBJ
CAB36701.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161585
EMBL· GenBank· DDBJ
CAB80141.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE86347.1
EMBL· GenBank· DDBJ
Genomic DNA
AY054633
EMBL· GenBank· DDBJ
AAK96824.1
EMBL· GenBank· DDBJ
mRNA
AY081532
EMBL· GenBank· DDBJ
AAM10094.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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