Q8VZR0 · RIBF2_ARATH
- ProteinFAD synthetase 2, chloroplastic
- GeneRIBF2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids367 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.
Catalytic activity
- FMN + ATP + H+ = FAD + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
20.8 μM | FMN | |||||
13.1 μM | ATP |
kcat is 1.28x10-1 sec-1 with FMN as substrate (PubMed:18713732).
kcat is 1.28x10-1 sec-1 with ATP as substrate (PubMed:18713732).
kcat is 1.28x10-1 sec-1 with ATP as substrate (PubMed:18713732).
Pathway
Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | ATP binding | |
Molecular Function | FMN adenylyltransferase activity | |
Biological Process | FAD biosynthetic process | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFAD synthetase 2, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8VZR0
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transit peptide | 1-57 | Chloroplast | |||
Chain | PRO_0000429027 | 58-367 | FAD synthetase 2, chloroplastic | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q8VZR0-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length367
- Mass (Da)40,528
- Last updated2002-03-01 v1
- Checksum9278AC6D8152EA93
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4KB09 | F4KB09_ARATH | At5g08340 | 351 |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
EU687458 EMBL· GenBank· DDBJ | ACH56224.1 EMBL· GenBank· DDBJ | mRNA | ||
AL392174 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002688 EMBL· GenBank· DDBJ | AED91286.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY063929 EMBL· GenBank· DDBJ | AAL36285.1 EMBL· GenBank· DDBJ | mRNA | ||
AY091253 EMBL· GenBank· DDBJ | AAM14192.1 EMBL· GenBank· DDBJ | mRNA |