Q8VYB2 · UKL3_ARATH
- ProteinUridine kinase-like protein 3
- GeneUKL3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids466 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Involved in the pyrimidine salvage pathway. The uracil phosphoribosyltransferase (UPRT) activity, that catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate, is unsure.
Catalytic activity
- UMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + uracil
Cofactor
Note: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.
Activity regulation
Allosterically activated by GTP.
Pathway
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.
Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 280 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 289 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 323-326 | GTP (UniProtKB | ChEBI) | ||||
Sequence: CKKL | ||||||
Binding site | 333 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 358 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 378 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 384 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 389-392 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI) | ||||
Sequence: TGNS | ||||||
Binding site | 454-456 | uracil (UniProtKB | ChEBI) | ||||
Sequence: GEF | ||||||
Binding site | 455 | 5-phospho-alpha-D-ribose 1-diphosphate (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | cytidine kinase activity | |
Molecular Function | GTP binding | |
Molecular Function | uracil phosphoribosyltransferase activity | |
Molecular Function | uridine kinase activity | |
Biological Process | cellular response to hypoxia | |
Biological Process | CTP salvage | |
Biological Process | phosphorylation | |
Biological Process | UMP salvage |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUridine kinase-like protein 3
Including 2 domains:
- Recommended nameUridine kinase
- EC number
- Short namesUK
- Recommended namePutative uracil phosphoribosyltransferase
- EC number
- Short namesUPRTase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8VYB2
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
No visible phenotype. No decrease in uracil phosphoribosyltransferase activity.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 24 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000394516 | 1-466 | Uridine kinase-like protein 3 | |||
Sequence: MASKSDVNIIETSSKVHFSGFHQMDGLASNRPEQMAEEEEHGQPFVIGVAGGAASGKTTVCDMIMQQLHDQRAVVVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNYDFKSYKNNVFPPRRVNPSDVIILEGILIFHDPRVRDLMNMKIFVDADADVRLARRIKRDTVEKGRDIATVLDQYSKFVKPAFEDFILPTKKYADIIIPRGGDNHVAIDLIVQHIHTKLGQHDLCKIYPNLYVIQSTFQIRGMHTLIRDSKTTKHDFIFYSDRLIRLVVEHGLGHLPFTEKQVVTPTGSVYSGVDFCKKLCGVSVIRSGESMENALRACCKGIKIGKILIHREGDNGQQLIYEKLPSDISERHVLLLDPILGTGNSAVQAIRLLISKGVPESNIIFLNLISAPEGVNVVCKKFPRIKIVTSEIELGLNDEFRVVPGMGEFGDRYFGTDDE |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 41-246 | Uridine kinase | ||||
Sequence: HGQPFVIGVAGGAASGKTTVCDMIMQQLHDQRAVVVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNYDFKSYKNNVFPPRRVNPSDVIILEGILIFHDPRVRDLMNMKIFVDADADVRLARRIKRDTVEKGRDIATVLDQYSKFVKPAFEDFILPTKKYADIIIPRGGDNHVAIDLIVQHIHTKLG | ||||||
Region | 256-466 | Uracil phosphoribosyltransferase | ||||
Sequence: NLYVIQSTFQIRGMHTLIRDSKTTKHDFIFYSDRLIRLVVEHGLGHLPFTEKQVVTPTGSVYSGVDFCKKLCGVSVIRSGESMENALRACCKGIKIGKILIHREGDNGQQLIYEKLPSDISERHVLLLDPILGTGNSAVQAIRLLISKGVPESNIIFLNLISAPEGVNVVCKKFPRIKIVTSEIELGLNDEFRVVPGMGEFGDRYFGTDDE |
Sequence similarities
In the N-terminal section; belongs to the uridine kinase family.
In the C-terminal section; belongs to the UPRTase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8VYB2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length466
- Mass (Da)52,443
- Last updated2002-03-01 v1
- Checksum3FA6783CB49727F7
Q8VYB2-2
- Name2
- Differences from canonical
- 366-370: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AWM1 | A0A1P8AWM1_ARATH | UKL3 | 488 | ||
A0A1P8AWK0 | A0A1P8AWK0_ARATH | UKL3 | 451 |
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_039280 | 366-370 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC002304 EMBL· GenBank· DDBJ | AAF79310.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE33300.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE33301.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE33302.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | ANM61033.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY072218 EMBL· GenBank· DDBJ | AAL60039.1 EMBL· GenBank· DDBJ | mRNA | ||
AY122946 EMBL· GenBank· DDBJ | AAM67479.1 EMBL· GenBank· DDBJ | mRNA | ||
AK317232 EMBL· GenBank· DDBJ | BAH19913.1 EMBL· GenBank· DDBJ | mRNA |