Q8VY10 · UBC24_ARATH

Function

function

E2 ubiquitin-protein ligase that mediates E1-dependent protein ubiquitination (PubMed:24474629).
Mediates PHO1 degradation through multivesicular body-mediated vacuolar proteolysis in response to inorganic phosphate (Pi) availability (PubMed:22634761).
Negatively regulates the protein abundance of PHF1 and PHT1s under Pi-sufficient conditions by facilitating the degradation of PHT1 proteins at the endomembrane (PubMed:22634761, PubMed:24122829).
Functions cooperatively with NLA to regulate the abundance of the inorganic phosphate (Pi) transporters PHT1-1, PHT1-2 and PHT1-3 in different subcellular compartments (PubMed:24122828).
Regulates Pi homeostasis by mediating, cooperatively with NLA, polyubiquitination of PHT1-4 and its targeting for degradation (PubMed:24474629).

Miscellaneous

MicroRNA399 (miR399) can be sequestered by IPS1, a non-protein coding RNA containing a motif with sequence complementarity to miR399, but with a mismatched loop at the expected miRNA cleavage site. Thus IPS1 mimics the target of miR399 to block the cleavage of UBC24/PHO2 under Pi-deficient conditions.

Catalytic activity

  • S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
    EC:2.3.2.23 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site748Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi membrane
Molecular FunctionATP binding
Molecular Functionligase activity
Molecular Functionubiquitin conjugating enzyme activity
Biological Processcellular response to phosphate starvation
Biological Processphosphate ion homeostasis
Biological Processphosphate ion transport
Biological Processprotein ubiquitination
Biological Processregulation of phosphate transmembrane transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable ubiquitin-conjugating enzyme E2 24
  • EC number
  • Alternative names
    • AtPHO2
    • E2 ubiquitin-conjugating enzyme 24
    • Ubiquitin carrier protein 24
    • Ubiquitin-protein ligase 24

Gene names

    • Name
      UBC24
    • Synonyms
      PHO2
    • ORF names
      T1B8.8
    • Ordered locus names
      At2g33770

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8VY10
  • Secondary accessions
    • P93012
    • Q0WLN6
    • Q0WM96

Proteomes

Organism-specific databases

Genome annotation databases

Phenotypes & Variants

Disruption phenotype

Plants are unable to regulate the amount of phosphate accumulated into shoots.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis748Loss of ubiquitin conjugase activity and loss of down-regulation of PHO1.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 95 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003443611-907Probable ubiquitin-conjugating enzyme E2 24

Proteomic databases

Expression

Tissue specificity

Expressed in the vascular tissues of cotyledons, leaves, roots, sepals, filaments, anthers and junctions between the inflorescence stems and siliques.

Induction

Down-regulated by phosphate deprivation (PubMed:16679424).
Systemically regulated by microRNA399 (miR399) (PubMed:16679424, PubMed:18390805).

Developmental stage

Up-regulated in senescing leaves and maturating seeds.

Gene expression databases

Interaction

Subunit

Interacts with PHO1 (PubMed:22634761).
Interacts with NLA (PubMed:24474629).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-23Disordered
Region485-509Disordered
Domain662-822UBC core

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q8VY10-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    907
  • Mass (Da)
    100,484
  • Last updated
    2002-03-01 v1
  • Checksum
    6C9CB1D8A3A95359
MEMSLTDSDWDSSSDSGSSEHEEVEFSYGGRAQNIFSNLEETIGKIDEFLSFERGFMYGDIVRSATEPSGQSGRVINIDMFVNLESTHGKIMKEVDTKRLQKLRSISLSDYVINGPWVGRVDKIVERVSVTLDDGTNYEVLVDGQDKLVAIPPNLLEDSQYSYYPGQRVQVKLAHAPRSTTWLCGTWRGTQVMGTVCTVEAGLVYVDWVASIVMEGDRNLTAPQALQNPESLTLLPCVSHASWQLGDWCILPGSSHCDIAERQTPNVAAYNLNECHKTFQKGFNRNMQNSGLDELFVITKTKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCNSKKWGVVKAVNAKEQTVKVQWTIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKMERSEYSNSSTVSSEGSVQDLSQKISQSDEASSNHQETGLVKLYSVGESCNENIPECSSFFLPKAAIGFITNLASSLFGYQGSTSVISSHSRCNDSEDQSDSEVLVQETAESYDNSETNSGEVDMTTTMVNIPIEGKGINKTLDSTLLENSRNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSANLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGVDCVQEIGPES

Q8VY10-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence AAC69130.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0347511-476in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ027037
EMBL· GenBank· DDBJ
AAY44863.1
EMBL· GenBank· DDBJ
mRNA
U78721
EMBL· GenBank· DDBJ
AAC69130.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002685
EMBL· GenBank· DDBJ
AEC08882.1
EMBL· GenBank· DDBJ
Genomic DNA
AY074292
EMBL· GenBank· DDBJ
AAL66989.1
EMBL· GenBank· DDBJ
mRNA
AY091326
EMBL· GenBank· DDBJ
AAM14265.1
EMBL· GenBank· DDBJ
mRNA
AK229934
EMBL· GenBank· DDBJ
BAF01760.1
EMBL· GenBank· DDBJ
mRNA
AK230162
EMBL· GenBank· DDBJ
BAF01971.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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