Q8VSP9 · OSPF_SHIFL
- ProteinPhosphothreonine lyase OspF
- GeneospF
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids239 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the removal of the phosphate group from the phosphothreonine in the mitogen-activated protein kinases such as MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction, thus preventing the downstream phosphorylation of histone H3. This epigenetic modification results in inhibition of the transcription of a specific subset of pro-inflammatory genes, and ultimately to a reduced immune response against the invading pathogen. The diminished immune response enhances the bacterium's ability to disseminate and multiply within the host.
Activity regulation
Inhibited by the tyrosine phosphatase inhibitor vanadate.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
204 nM | phosphorylated MAPK2 | 8 | 30 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
9.89 pM/sec/ug | 8 | 30 | with phosphorylated MAPK2 as substrate |
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 104 | Proton donor | ||||
Sequence: H | ||||||
Active site | 134 | Proton acceptor | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | lyase activity |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphothreonine lyase OspF
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid pWR100
- Plasmid pWR501
- Plasmid pCP301
- Plasmid pSF5
- Plasmid pINV_F6_M1382
- Plasmid pSf2b_251
- Plasmid pSf1a_571
- Plasmid pSfx_580
- Plasmid pSf6_579
- Plasmid pSf3_575
- Plasmid pSf4a_576
- Plasmid pSf4b_577
- Plasmid pSf5_246
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella
Accessions
- Primary accessionQ8VSP9
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Secreted via the type III secretion system (T3SS). Localizes in the nucleus of the infected cell.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 19 | in plasmid pWR100, plasmid pWR501 and plasmid pSF5 | ||||
Sequence: R → K | ||||||
Natural variant | 66 | in plasmid pWR100, plasmid pWR501, plasmid pSF5, plasmid pINV_F6_M1382, plasmid pSf6_579, plasmid pSf3_575, plasmid pSf4a_576, plasmid pSf4b_577 and plasmid pSf5_246 | ||||
Sequence: S → F | ||||||
Mutagenesis | 101 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 102 | Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. | ||||
Sequence: K → R | ||||||
Mutagenesis | 104 | Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. | ||||
Sequence: H → A | ||||||
Mutagenesis | 127 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 131 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 134 | Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling. | ||||
Sequence: K → A | ||||||
Mutagenesis | 137 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 160 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 175 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 218 | No change in Erk2 phosphothreonine lyase activity. | ||||
Sequence: R → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000299354 | 1-239 | Phosphothreonine lyase OspF | |||
Sequence: MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIRQYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDKFHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSDQECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGSERQEQMLREEPFYRLMIE |
Proteomic databases
Expression
Induction
Transcriptionally activated by MxiE in the intracellular environment of the host, in association with IpgC, under conditions of deregulated or active secretion. Expressed in a VirB-dependent but MxiE-independent way under conditions of non-secretion.
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q8VSP9 | MAPK1 P28482 | 5 | EBI-6506625, EBI-959949 | |
XENO | Q8VSP9 | RB1 P06400 | 2 | EBI-6506625, EBI-491274 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length239
- Mass (Da)27,828
- Last updated2002-03-01 v1
- Checksum75F6EA2D6FFD002A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL391753 EMBL· GenBank· DDBJ | CAC05773.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF348706 EMBL· GenBank· DDBJ | AAK18324.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY206429 EMBL· GenBank· DDBJ | AAP78969.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY879342 EMBL· GenBank· DDBJ | AAW64770.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF386526 EMBL· GenBank· DDBJ | AAL72315.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362850 EMBL· GenBank· DDBJ | ABD37257.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362864 EMBL· GenBank· DDBJ | ABD37271.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362865 EMBL· GenBank· DDBJ | ABD37272.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362866 EMBL· GenBank· DDBJ | ABD37273.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362867 EMBL· GenBank· DDBJ | ABD37274.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362868 EMBL· GenBank· DDBJ | ABD37275.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362869 EMBL· GenBank· DDBJ | ABD37276.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DQ362870 EMBL· GenBank· DDBJ | ABD37277.1 EMBL· GenBank· DDBJ | Genomic DNA |