Q8VSP9 · OSPF_SHIFL

  • Protein
    Phosphothreonine lyase OspF
  • Gene
    ospF
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the removal of the phosphate group from the phosphothreonine in the mitogen-activated protein kinases such as MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction, thus preventing the downstream phosphorylation of histone H3. This epigenetic modification results in inhibition of the transcription of a specific subset of pro-inflammatory genes, and ultimately to a reduced immune response against the invading pathogen. The diminished immune response enhances the bacterium's ability to disseminate and multiply within the host.

Activity regulation

Inhibited by the tyrosine phosphatase inhibitor vanadate.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
204 nMphosphorylated MAPK2830
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
9.89 pM/sec/ug830with phosphorylated MAPK2 as substrate

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site104Proton donor
Active site134Proton acceptor

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionlyase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphothreonine lyase OspF
  • EC number
  • Alternative names
    • Effector protein OspF

Gene names

    • Name
      ospF
    • Synonyms
      mkaD
    • ORF names
      pWR501_0013, SFLP011
    • Ordered locus names
      CP0010

Encoded on

  • Plasmid pWR100
  • Plasmid pWR501
  • Plasmid pCP301
  • Plasmid pSF5
  • Plasmid pINV_F6_M1382
  • Plasmid pSf2b_251
  • Plasmid pSf1a_571
  • Plasmid pSfx_580
  • Plasmid pSf6_579
  • Plasmid pSf3_575
  • Plasmid pSf4a_576
  • Plasmid pSf4b_577
  • Plasmid pSf5_246

Organism names

  • Taxonomic identifier
  • Strains
    • 580
    • M90T / Serotype 5a
    • M1382 / Serotype 6
    • Serotype 5
    • 301 / Serotype 2a
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Shigella

Accessions

  • Primary accession
    Q8VSP9
  • Secondary accessions
    • Q2ET10
    • Q2ET14
    • Q6XW10
    • Q7BEG0
    • Q99Q87

Proteomes

Subcellular Location

Secreted
Note: Secreted via the type III secretion system (T3SS). Localizes in the nucleus of the infected cell.

Keywords

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant19in plasmid pWR100, plasmid pWR501 and plasmid pSF5
Natural variant66in plasmid pWR100, plasmid pWR501, plasmid pSF5, plasmid pINV_F6_M1382, plasmid pSf6_579, plasmid pSf3_575, plasmid pSf4a_576, plasmid pSf4b_577 and plasmid pSf5_246
Mutagenesis101No change in Erk2 phosphothreonine lyase activity.
Mutagenesis102Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling.
Mutagenesis104Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling.
Mutagenesis127No change in Erk2 phosphothreonine lyase activity.
Mutagenesis131No change in Erk2 phosphothreonine lyase activity.
Mutagenesis134Loss of Erk2 phosphothreonine lyase activity. No effect in binding ability to erk. Deficient in down-regulating MAPK signaling.
Mutagenesis137No change in Erk2 phosphothreonine lyase activity.
Mutagenesis160No change in Erk2 phosphothreonine lyase activity.
Mutagenesis175No change in Erk2 phosphothreonine lyase activity.
Mutagenesis218No change in Erk2 phosphothreonine lyase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 2 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002993541-239Phosphothreonine lyase OspF

Proteomic databases

Expression

Induction

Transcriptionally activated by MxiE in the intracellular environment of the host, in association with IpgC, under conditions of deregulated or active secretion. Expressed in a VirB-dependent but MxiE-independent way under conditions of non-secretion.

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO Q8VSP9MAPK1 P284825EBI-6506625, EBI-959949
XENO Q8VSP9RB1 P064002EBI-6506625, EBI-491274

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the phosphothreonine lyase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    239
  • Mass (Da)
    27,828
  • Last updated
    2002-03-01 v1
  • Checksum
    75F6EA2D6FFD002A
MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIRQYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDKFHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSDQECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGSERQEQMLREEPFYRLMIE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL391753
EMBL· GenBank· DDBJ
CAC05773.1
EMBL· GenBank· DDBJ
Genomic DNA
AF348706
EMBL· GenBank· DDBJ
AAK18324.1
EMBL· GenBank· DDBJ
Genomic DNA
AY206429
EMBL· GenBank· DDBJ
AAP78969.1
EMBL· GenBank· DDBJ
Genomic DNA
AY879342
EMBL· GenBank· DDBJ
AAW64770.1
EMBL· GenBank· DDBJ
Genomic DNA
AF386526
EMBL· GenBank· DDBJ
AAL72315.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362850
EMBL· GenBank· DDBJ
ABD37257.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362864
EMBL· GenBank· DDBJ
ABD37271.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362865
EMBL· GenBank· DDBJ
ABD37272.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362866
EMBL· GenBank· DDBJ
ABD37273.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362867
EMBL· GenBank· DDBJ
ABD37274.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362868
EMBL· GenBank· DDBJ
ABD37275.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362869
EMBL· GenBank· DDBJ
ABD37276.1
EMBL· GenBank· DDBJ
Genomic DNA
DQ362870
EMBL· GenBank· DDBJ
ABD37277.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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