Q8VHK0 · ACOT8_RAT
- ProteinAcyl-coenzyme A thioesterase 8
- GeneAcot8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids320 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:11785945).
Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity).
Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:11785945).
Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity).
Is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity).
It is involved in the metabolic regulation of peroxisome proliferation (By similarity).
Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity).
Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:11785945).
Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity).
Is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity).
It is involved in the metabolic regulation of peroxisome proliferation (By similarity).
Catalytic activity
- choloyl-CoA + H2O = cholate + CoA + H+This reaction proceeds in the forward direction.
- butanoyl-CoA + H2O = butanoate + CoA + H+This reaction proceeds in the forward direction.
- H2O + hexanoyl-CoA = CoA + H+ + hexanoateThis reaction proceeds in the forward direction.
- H2O + octanoyl-CoA = CoA + H+ + octanoateThis reaction proceeds in the forward direction.
- decanoyl-CoA + H2O = CoA + decanoate + H+This reaction proceeds in the forward direction.
- dodecanoyl-CoA + H2O = CoA + dodecanoate + H+This reaction proceeds in the forward direction.
- H2O + tetradecanoyl-CoA = CoA + H+ + tetradecanoateThis reaction proceeds in the forward direction.
- 4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA + H+This reaction proceeds in the forward direction.
- 2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA + H+This reaction proceeds in the forward direction.
- H2O + malonyl-CoA = CoA + H+ + malonateThis reaction proceeds in the forward direction.
- H2O + propanoyl-CoA = CoA + H+ + propanoateThis reaction proceeds in the forward direction.
- glutaryl-CoA + H2O = CoA + glutarate + H+This reaction proceeds in the forward direction.
- H2O + hexanedioyl-CoA = CoA + H+ + hexanedioateThis reaction proceeds in the forward direction.
- H2O + octanedioyl-CoA = CoA + H+ + octanedioateThis reaction proceeds in the forward direction.
- decanedioyl-CoA + H2O = CoA + decanedioate + H+This reaction proceeds in the forward direction.
- dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H+This reaction proceeds in the forward direction.
- (9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA + H+This reaction proceeds in the forward direction.
- (9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H+This reaction proceeds in the forward direction.
- H2O + octadecanoyl-CoA = CoA + H+ + octadecanoateThis reaction proceeds in the forward direction.
- (9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H+This reaction proceeds in the forward direction.
- (9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H+This reaction proceeds in the forward direction.
- eicosanoyl-CoA + H2O = CoA + eicosanoate + H+This reaction proceeds in the forward direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H+This reaction proceeds in the forward direction.
- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA + H+This reaction proceeds in the forward direction.
- 2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA + H+This reaction proceeds in the forward direction.
- H2O + prostaglandin F2alpha-CoA = CoA + H+ + prostaglandin F2alphaThis reaction proceeds in the forward direction.
Activity regulation
Inhibited by CoASH (IC50=10-15 uM). Also inhibited by cysteine-reactive agents.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 233 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 255 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 305 | Charge relay system | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | peroxisomal matrix | |
Cellular Component | peroxisome | |
Molecular Function | acetoacetyl-CoA hydrolase activity | |
Molecular Function | acetyl-CoA hydrolase activity | |
Molecular Function | carboxylic ester hydrolase activity | |
Molecular Function | choloyl-CoA hydrolase activity | |
Molecular Function | fatty acyl-CoA hydrolase activity | |
Molecular Function | hydroxymethylglutaryl-CoA hydrolase activity | |
Molecular Function | long-chain fatty acyl-CoA hydrolase activity | |
Molecular Function | medium-chain fatty acyl-CoA hydrolase activity | |
Molecular Function | succinyl-CoA hydrolase activity | |
Biological Process | acyl-CoA metabolic process | |
Biological Process | dicarboxylic acid catabolic process | |
Biological Process | fatty acid catabolic process | |
Biological Process | negative regulation of CD4 production | |
Biological Process | peroxisome fission |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameAcyl-coenzyme A thioesterase 8
- EC number
- Short namesAcyl-CoA thioesterase 8
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ8VHK0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000202154 | 1-320 | Acyl-coenzyme A thioesterase 8 | |||
Sequence: MSKPEDLGDANGDADRGDLSGDLRSVLVTSVLNLEPLDEDLYRGRHYWVPTSQRLFGGQIVGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKVPVLYHVERTRTGASFSVRAVKAVQHGKAIFICQASFQQMQPSPLQHQFSMPTVPPPEELLDHEALIDQYLRDPNLHEKYRVGLNRIAAREVPIEIKLVNPPALNQLQTLEPKQMFWVRARGYIGEGDIKMHCCVAAYISDYAFLGTALLPHQSKYKVNFMVSLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRRDGVLAVTCAQEGVIRSKPRVSESKL |
Proteomic databases
PTM databases
Interaction
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 318-320 | Microbody targeting signal | ||||
Sequence: SKL |
Sequence similarities
Belongs to the C/M/P thioester hydrolase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length320
- Mass (Da)36,003
- Last updated2002-03-01 v1
- ChecksumE0EAB4B7D26C79C0
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F7F557 | F7F557_RAT | Acot8 | 374 | ||
A0A8I5Y0F4 | A0A8I5Y0F4_RAT | Acot8 | 109 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF452100 EMBL· GenBank· DDBJ | AAL66289.1 EMBL· GenBank· DDBJ | mRNA |