Q8VEG6 · CNO6L_MOUSE

  • Protein
    CCR4-NOT transcription complex subunit 6-like
  • Gene
    Cnot6l
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).

Catalytic activity

  • Exonucleolytic cleavage of poly(A) to 5'-AMP.
    EC:3.1.13.4 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions, but the ions interact each with only 1 or 2 residues.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site240Mg2+ 1 (UniProtKB | ChEBI)
Binding site240substrate
Binding site276substrate
Binding site360substrate
Binding site365substrate
Active site410Proton donor/acceptor
Binding site410Mg2+ 2 (UniProtKB | ChEBI)
Binding site412substrate
Binding site479substrate
Binding site484substrate

GO annotations

AspectTerm
Cellular ComponentCCR4-NOT complex
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionmetal ion binding
Molecular Functionpoly(A)-specific ribonuclease activity
Biological ProcessmRNA destabilization
Biological ProcessmRNA processing
Biological Processnuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Processnuclear-transcribed mRNA poly(A) tail shortening
Biological Processpositive regulation of cell population proliferation
Biological Processpositive regulation of cytoplasmic mRNA processing body assembly
Biological Processregulation of translation
Biological Processregulatory ncRNA-mediated gene silencing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CCR4-NOT transcription complex subunit 6-like
  • EC number

Gene names

    • Name
      Cnot6l

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
    • OF1
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8VEG6
  • Secondary accessions
    • Q3U9M0
    • Q8C0P4

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: Predominantly cytoplasmic.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003145881-555CCR4-NOT transcription complex subunit 6-like

Proteomic databases

PTM databases

Expression

Developmental stage

Expressed in embryonic stem (ES) cells.

Gene expression databases

Interaction

Subunit

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By similarity).
Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (By similarity).
Interacts with TOB1 (By similarity).
Interacts with NANOS2 (PubMed:20133598).
Interacts with ZFP36 (PubMed:21078877).
Interacts with ZFP36L2 (By similarity).
Interacts with RBM46 (PubMed:36001654).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q8VEG6Cnot7 Q608092EBI-2104661, EBI-2104739

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, repeat.

TypeIDPosition(s)Description
Region1-152Required for interaction with CNOT1, CNOT3 and CNOT7
Repeat57-78LRR 1
Repeat80-101LRR 2
Repeat103-125LRR 3
Repeat126-148LRR 4
Region158-555Nuclease domain

Sequence similarities

Belongs to the CCR4/nocturin family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q8VEG6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    555
  • Mass (Da)
    63,023
  • Last updated
    2008-01-15 v2
  • Checksum
    FDC180DB315919DB
MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLARIPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR

Q8VEG6-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-6: MRLIGM → M

Q8VEG6-3

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F8WJG1F8WJG1_MOUSECnot6l46
D3YXZ0D3YXZ0_MOUSECnot6l130
D3Z5R3D3Z5R3_MOUSECnot6l55
D3YVH6D3YVH6_MOUSECnot6l99
D3Z2G5D3Z2G5_MOUSECnot6l87

Sequence caution

The sequence BAC26790.1 differs from that shown. Reason: Frameshift

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0303241-6in isoform 2 and isoform 3
Sequence conflict238in Ref. 1; BAE30646
Alternative sequenceVSP_030325440in isoform 3
Alternative sequenceVSP_030326441-555in isoform 3

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK030112
EMBL· GenBank· DDBJ
BAC26790.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AK151728
EMBL· GenBank· DDBJ
BAE30646.1
EMBL· GenBank· DDBJ
mRNA
AC129600
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AC149285
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC018506
EMBL· GenBank· DDBJ
AAH18506.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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