Q8VEG6 · CNO6L_MOUSE
- ProteinCCR4-NOT transcription complex subunit 6-like
- GeneCnot6l
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids555 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).
Catalytic activity
Cofactor
Note: Binds 2 magnesium ions, but the ions interact each with only 1 or 2 residues.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 240 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 240 | substrate | ||||
Sequence: E | ||||||
Binding site | 276 | substrate | ||||
Sequence: E | ||||||
Binding site | 360 | substrate | ||||
Sequence: H | ||||||
Binding site | 365 | substrate | ||||
Sequence: P | ||||||
Active site | 410 | Proton donor/acceptor | ||||
Sequence: D | ||||||
Binding site | 410 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 412 | substrate | ||||
Sequence: N | ||||||
Binding site | 479 | substrate | ||||
Sequence: N | ||||||
Binding site | 484 | substrate | ||||
Sequence: F |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | CCR4-NOT complex | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | poly(A)-specific ribonuclease activity | |
Biological Process | mRNA destabilization | |
Biological Process | mRNA processing | |
Biological Process | nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay | |
Biological Process | nuclear-transcribed mRNA poly(A) tail shortening | |
Biological Process | positive regulation of cell population proliferation | |
Biological Process | positive regulation of cytoplasmic mRNA processing body assembly | |
Biological Process | regulation of translation | |
Biological Process | regulatory ncRNA-mediated gene silencing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCCR4-NOT transcription complex subunit 6-like
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8VEG6
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000314588 | 1-555 | CCR4-NOT transcription complex subunit 6-like | |||
Sequence: MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLARIPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR |
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed in embryonic stem (ES) cells.
Gene expression databases
Interaction
Subunit
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By similarity).
Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (By similarity).
Interacts with TOB1 (By similarity).
Interacts with NANOS2 (PubMed:20133598).
Interacts with ZFP36 (PubMed:21078877).
Interacts with ZFP36L2 (By similarity).
Interacts with RBM46 (PubMed:36001654).
Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (By similarity).
Interacts with TOB1 (By similarity).
Interacts with NANOS2 (PubMed:20133598).
Interacts with ZFP36 (PubMed:21078877).
Interacts with ZFP36L2 (By similarity).
Interacts with RBM46 (PubMed:36001654).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8VEG6 | Cnot7 Q60809 | 2 | EBI-2104661, EBI-2104739 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-152 | Required for interaction with CNOT1, CNOT3 and CNOT7 | ||||
Sequence: MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLARIPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLYQDPDGTR | ||||||
Repeat | 57-78 | LRR 1 | ||||
Sequence: HLTALHLNDNNLARIPPDIAKL | ||||||
Repeat | 80-101 | LRR 2 | ||||
Sequence: NLVYLDLSSNKLRSLPAELGNM | ||||||
Repeat | 103-125 | LRR 3 | ||||
Sequence: SLRELLLNDNYLRVLPYELGRLF | ||||||
Repeat | 126-148 | LRR 4 | ||||
Sequence: QLQTLGLTGNPLSQDIMSLYQDP | ||||||
Region | 158-555 | Nuclease domain | ||||
Sequence: MLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR |
Sequence similarities
Belongs to the CCR4/nocturin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q8VEG6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length555
- Mass (Da)63,023
- Last updated2008-01-15 v2
- ChecksumFDC180DB315919DB
Q8VEG6-2
- Name2
- Differences from canonical
- 1-6: MRLIGM → M
Q8VEG6-3
- Name3
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK030112 EMBL· GenBank· DDBJ | BAC26790.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK151728 EMBL· GenBank· DDBJ | BAE30646.1 EMBL· GenBank· DDBJ | mRNA | ||
AC129600 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC149285 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC018506 EMBL· GenBank· DDBJ | AAH18506.1 EMBL· GenBank· DDBJ | mRNA |