Q8VBW6 · ULA1_MOUSE

  • Protein
    NEDD8-activating enzyme E1 regulatory subunit
  • Gene
    Nae1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation (By similarity).
The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development

Activity regulation

Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity.

Pathway

Protein modification; protein neddylation.

Features

Showing features for site.

153450100150200250300350400450500
TypeIDPosition(s)Description
Site211Interaction with UBA3

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentglutamatergic synapse
Cellular Componentplasma membrane
Cellular Componentprotein-containing complex
Molecular FunctionNEDD8 activating enzyme activity
Molecular Functionprotein heterodimerization activity
Molecular Functionubiquitin protein ligase binding
Biological Processmitotic DNA replication checkpoint signaling
Biological Processneuron apoptotic process
Biological Processprotein neddylation
Biological Processregulation of neuron apoptotic process
Biological Processregulation of postsynapse assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NEDD8-activating enzyme E1 regulatory subunit
  • Alternative names
    • Amyloid beta precursor protein-binding protein 1, 59 kDa (APP-BP1)
    • Amyloid protein-binding protein 1

Gene names

    • Name
      Nae1
    • Synonyms
      Appbp1

Organism names

  • Taxonomic identifier
  • Strain
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8VBW6
  • Secondary accessions
    • Q8CFL6

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00001949532-534NEDD8-activating enzyme E1 regulatory subunit
Modified residue6N6-acetyllysine
Modified residue341N6-acetyllysine

Post-translational modification

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region331-344Interaction with UBA3

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    534
  • Mass (Da)
    60,274
  • Last updated
    2002-03-01 v1
  • Checksum
    5CFCABFE5FA7AC62
MAQPGKILKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNLVSGEDAGNNFFLQKSSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHLQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYNETNGRIPKSYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSNKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
E0CZE0E0CZE0_MOUSENae1510

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict127in Ref. 1; AAH23680

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC019163
EMBL· GenBank· DDBJ
AAH19163.1
EMBL· GenBank· DDBJ
mRNA
BC021510
EMBL· GenBank· DDBJ
AAH21510.1
EMBL· GenBank· DDBJ
mRNA
BC023680
EMBL· GenBank· DDBJ
AAH23680.1
EMBL· GenBank· DDBJ
mRNA
BC023897
EMBL· GenBank· DDBJ
AAH23897.1
EMBL· GenBank· DDBJ
mRNA
BC027124
EMBL· GenBank· DDBJ
AAH27124.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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