Q8UIN4 · SYFB_AGRFC
- ProteinPhenylalanine--tRNA ligase beta subunit
- GenepheT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids807 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H+ + L-phenylalanyl-tRNA(Phe)
Cofactor
Note: Binds 2 magnesium ions per tetramer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 453 | Mg2+ (UniProtKB | ChEBI); shared with alpha subunit | ||||
Sequence: D | ||||||
Binding site | 459 | Mg2+ (UniProtKB | ChEBI); shared with alpha subunit | ||||
Sequence: D | ||||||
Binding site | 462 | Mg2+ (UniProtKB | ChEBI); shared with alpha subunit | ||||
Sequence: E | ||||||
Binding site | 463 | Mg2+ (UniProtKB | ChEBI); shared with alpha subunit | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | phenylalanine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Biological Process | phenylalanyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhenylalanine--tRNA ligase beta subunit
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Agrobacterium > Agrobacterium tumefaciens complex
Accessions
- Primary accessionQ8UIN4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000126831 | 1-807 | Phenylalanine--tRNA ligase beta subunit | |||
Sequence: MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADRLKVLMVDAGDGKPVQIVCGAPNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMCSEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEINLTPNRPDCTSIYGIARDLAASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLMAIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGPNNVVIADEKGLESIGGVMGGEHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYRFERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGHQPKVVDFPLSEVKRLTGLEVSSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLENFGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAADMSDMRPSLLPGLLTAAQRNADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLAGSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANVQIEAGAPAWYHPGRSGTIKMGPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKRDFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKTLTDEDFEALTAKIVGNVEKSTGGVLRA |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 38-148 | tRNA-binding | ||||
Sequence: KAAYKPFVIAKVVSAEKHPEADRLKVLMVDAGDGKPVQIVCGAPNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMCSEKELNISDDHDGIIDLPEDAPVGTSFA | ||||||
Domain | 400-475 | B5 | ||||
Sequence: HQPKVVDFPLSEVKRLTGLEVSSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKP | ||||||
Domain | 713-806 | FDX-ACB | ||||
Sequence: SPFQAVKRDFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKTLTDEDFEALTAKIVGNVEKSTGGVLR |
Sequence similarities
Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length807
- Mass (Da)86,357
- Last updated2002-06-01 v1
- Checksum0D36D17DD8F9CEB0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE007869 EMBL· GenBank· DDBJ | AAK86075.1 EMBL· GenBank· DDBJ | Genomic DNA |