Q8UEM4 · NRDJ_AGRFC
- ProteinVitamin B12-dependent ribonucleotide reductase
- GenenrdJ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1272 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen (By similarity).
Catalytic activity
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:73316 + CHEBI:15377 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:57930
Cofactor
Note: 5'-deoxyadenosylcobalamine (coenzyme B12).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 153 | substrate | ||||
Sequence: S | ||||||
Binding site | 198-199 | substrate | ||||
Sequence: AC | ||||||
Binding site | 230 | substrate | ||||
Sequence: G | ||||||
Active site | 474 | Proton acceptor | ||||
Sequence: N | ||||||
Binding site | 474-478 | substrate | ||||
Sequence: NPCSE | ||||||
Active site | 476 | Cysteine radical intermediate | ||||
Sequence: C | ||||||
Active site | 478 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 675-679 | substrate | ||||
Sequence: PTGTI |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | cobalamin binding | |
Molecular Function | cobalt ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor | |
Biological Process | DNA biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVitamin B12-dependent ribonucleotide reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Agrobacterium > Agrobacterium tumefaciens complex
Accessions
- Primary accessionQ8UEM4
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000231657 | 1-1272 | Vitamin B12-dependent ribonucleotide reductase | |||
Sequence: MRIERRFTKPGQSSYAEIEFRKAVSEIKNPDGSVVFRLADIDVPAQFSQVATDVLAQKYFRKAGVPKLLKKVEENDVPSFLWRSVADEKALKELPEAERYGSETDARQVFDRLAGTWAYWGWKGKYFSTEEDASAFKDELAYMLATQRVAPNSPQWFNTGLHWAYGIDGPGQGHFYVDPFTGKLTKSKSAYEHPQPHACFIQSVEDDLVNEGGIMDLWVREARLFKYGSGTGSNFSYLRGEGEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDADHPDIEAYIDWKVNEEQKVAALVTGSKIVAKHLKAIMKACVNCEADNGDCFDPAKNPALKREIRAAKKDMVPENYVKRVIQFAEQGYKDIQFKTYDTDWDSEAYLTVSGQNSNNSVSLKDDFLRAVENDGNWNLTARKDGKVMKTLKARDLWEKISHAAWASADPGLHFNTTMNDWHTSPAEGPIRASNPCSEYMFLDDTACNLASLNLLQFKDAKTKRIDIADYEHAVRLWTVVLEVSVMMAQFPSRQIAERSYEYRTLGLGYANIGGLLMSSGIPYDSDEGRAIAGALTAIMTGVSYATSAEMAGELGPFPSFAPNRDNMLRVIRNHRRAAHGQSEGYEGLSVNPVALIHADCTDQDLVAHATAAWDKALELGEKHGYRNAQTTVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPESLRSLGYSESQIAEIEAYAVGHGNLNQAPAINPSTLKAKGFTDEKIEAVNAALKSAFDIKFVFNQWTLGADFLKGTLKVSDEQLSDMSFNLLDHLGFAKKDIEAANVHVCGAMTLEGAPFLKNEHLAVFDCANPCGKIGKRYLSVESHIRMMAAAQPFISGAISKTINMPNDATVEDCGAAYMLSWKLALKANALYRDGSKLSQPLNASLVEDEDDEDFVEELIQQPLAQQAVTITEKIVERVIERVSREREKLPNRRQGYTQKATVGGHKVYLRTGEFGDGRIGEIFIDMHKEGAAFRAMMNNFAIAISLGLQYGVPLEEYVEAFTFTKFEPAGMVQGNDAIKNATSILDYVFRELAVSYLGRHDLAHVDTSDFSNTALGKGIQEGKTNLLSTGWTRGYKPTLVSSNEGDRAASEPKGSATAAPARGSANVTSFAGSAARKLEPTVAITTSEIVSFKRDYEERAKELAEEIAEEVIDEVVQEAQQTATALFSDKAAADAASAKAEAKKKENERRMRSIAQGYTGNMCSECQNFTMVRNGTCEKCDTCGATSGCS | ||||||
Disulfide bond | 199↔487 | Redox-active | ||||
Sequence: CFIQSVEDDLVNEGGIMDLWVREARLFKYGSGTGSNFSYLRGEGEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDADHPDIEAYIDWKVNEEQKVAALVTGSKIVAKHLKAIMKACVNCEADNGDCFDPAKNPALKREIRAAKKDMVPENYVKRVIQFAEQGYKDIQFKTYDTDWDSEAYLTVSGQNSNNSVSLKDDFLRAVENDGNWNLTARKDGKVMKTLKARDLWEKISHAAWASADPGLHFNTTMNDWHTSPAEGPIRASNPCSEYMFLDDTAC |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1120-1147 | Disordered | ||||
Sequence: TLVSSNEGDRAASEPKGSATAAPARGSA |
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase class-2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,272
- Mass (Da)139,623
- Last updated2002-06-01 v1
- Checksum3FA22DCC85AC6E2D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE007869 EMBL· GenBank· DDBJ | AAK87503.1 EMBL· GenBank· DDBJ | Genomic DNA |