Q8U4B6 · KAE1_PYRFU
- ProteintRNA N6-adenosine threonylcarbamoyltransferase
- Genekae1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids324 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
Catalytic activity
- L-threonylcarbamoyladenylate + adenosine37 in tRNA = N6-L-threonylcarbamoyladenosine37 in tRNA + AMP + H+
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 107 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 111 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 127 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 127-131 | substrate | |||
Binding site | 159 | substrate | |||
Binding site | 172 | substrate | |||
Binding site | 176 | substrate | |||
Binding site | 257 | substrate | |||
Binding site | 285 | Fe cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | EKC/KEOPS complex | |
Molecular Function | iron ion binding | |
Molecular Function | N(6)-L-threonylcarbamoyladenine synthase activity | |
Biological Process | tRNA threonylcarbamoyladenosine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA N6-adenosine threonylcarbamoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Pyrococcus
Accessions
- Primary accessionQ8U4B6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000303641 | 1-324 | tRNA N6-adenosine threonylcarbamoyltransferase | ||
Proteomic databases
Interaction
Subunit
Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length324
- Mass (Da)35,361
- Last updated2002-06-01 v1
- Checksum1C347CF45F76DDE2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE009950 EMBL· GenBank· DDBJ | AAL80296.1 EMBL· GenBank· DDBJ | Genomic DNA |