Q8TFD3 · DOTC_DOTSE
- ProteinEfflux pump dotC
- GenedotC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids585 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Efflux pump; part of the gene cluster that mediates the biosynthesis of dothistromin (DOTH), a polyketide toxin very similar in structure to the aflatoxin precursor, versicolorin B (PubMed:12039746, PubMed:17683963).
One function of dotC may be to transport early-stage dothistromin biosynthetic intermediates from the cytoplasm into vacuoles, thereby affecting the rate of dothistromin production (PubMed:22069539).
One function of dotC may be to transport early-stage dothistromin biosynthetic intermediates from the cytoplasm into vacuoles, thereby affecting the rate of dothistromin production (PubMed:22069539).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Cellular Component | vacuolar membrane | |
Molecular Function | transmembrane transporter activity |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameEfflux pump dotC
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetidae > Mycosphaerellales > Mycosphaerellaceae > Dothistroma
Accessions
- Primary accessionQ8TFD3
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Vacuole membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 51-71 | Helical | |||
Transmembrane | 94-114 | Helical | |||
Transmembrane | 132-152 | Helical | |||
Transmembrane | 158-178 | Helical | |||
Transmembrane | 186-206 | Helical | |||
Transmembrane | 214-234 | Helical | |||
Transmembrane | 247-267 | Helical | |||
Transmembrane | 280-300 | Helical | |||
Transmembrane | 323-343 | Helical | |||
Transmembrane | 353-373 | Helical | |||
Transmembrane | 385-405 | Helical | |||
Transmembrane | 414-434 | Helical | |||
Transmembrane | 449-471 | Helical | |||
Transmembrane | 524-544 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Decreases the expression of ver1, pksA and vbsA, and subsequent production of dothistromin, but does not affect the resistance to the toxin (PubMed:22069539).
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000443454 | 1-585 | Efflux pump dotC | ||
Glycosylation | 10 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 91 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
PTM databases
Expression
Induction
Expression is positively regulated by the dothistromin-specific transcription factor aflR (PubMed:23207690).
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-38 | Basic and acidic residues | |||
Region | 1-45 | Disordered | |||
Region | 564-585 | Disordered | |||
Sequence similarities
Belongs to the major facilitator superfamily. TCR/Tet family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length585
- Mass (Da)62,392
- Last updated2009-12-15 v2
- ChecksumCA55A4BBB7661099
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-38 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF448056 EMBL· GenBank· DDBJ | AAL87047.2 EMBL· GenBank· DDBJ | Genomic DNA |