Q8TB40 · ABHD4_HUMAN

  • Protein
    (Lyso)-N-acylphosphatidylethanolamine lipase
  • Gene
    ABHD4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.

Caution

Thr-291 is present instead of the conserved His which is expected to be an active site residue.

Catalytic activity

  • H2O + N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine = a fatty acid + H+ + N,1-diacyl-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-hexadecanoyl-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N-octadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-octadecanoyl-sn-glycero-3-phospho-ethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N-eicosanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-eicosanoyl-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.
  • 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H2O = (9Z)-octadecenoate + 1-octadecanoyl-2-hydroxy-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H+
    This reaction proceeds in the forward direction.
  • 1-O-(1Z-octadecenoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = (9Z)-octadecenoate + 1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H+
    This reaction proceeds in the forward direction.
  • H2O + N,1-diacyl-sn-glycero-3-phosphoethanolamine = a fatty acid + H+ + N-acyl-sn-glycero-3-phosphoethanolamine
    This reaction proceeds in the forward direction.

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentlipid droplet
Cellular Componentmitochondrion
Molecular Functionhydrolase activity
Molecular Functionlysophosphatidic acid acyltransferase activity
Molecular Functionlysophospholipase activity
Biological Processlipid catabolic process
Biological Processlipid homeostasis
Biological ProcessN-acylphosphatidylethanolamine metabolic process
Biological Processphosphatidic acid biosynthetic process
Biological Processphosphatidylethanolamine acyl-chain remodeling

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    (Lyso)-N-acylphosphatidylethanolamine lipase
  • EC number
  • Alternative names
    • Alpha/beta hydrolase domain-containing protein 4
      (Abhydrolase domain-containing protein 4
      )
    • Alpha/beta-hydrolase 4

Gene names

    • Name
      ABHD4

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    Q8TB40
  • Secondary accessions
    • B4DDH7
    • Q9H9E0

Proteomes

Organism-specific databases

Disease & Variants

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 367 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000808641-342(Lyso)-N-acylphosphatidylethanolamine lipase

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain70-201AB hydrolase-1

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q8TB40-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    342
  • Mass (Da)
    38,794
  • Last updated
    2002-06-01 v1
  • Checksum
    867EA9E0092559CB
MADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVAGPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD

Q8TB40-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 163-181: VKHLILVDPWGFPLRPTNP → AWSGAAIPAGLQTQVCRLL
    • 182-342: Missing

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
J3KMW0J3KMW0_HUMANABHD4149
H0YFD5H0YFD5_HUMANABHD454
F5GZC9F5GZC9_HUMANABHD444
F5H6T0F5H6T0_HUMANABHD490
F5H067F5H067_HUMANABHD485

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_056924163-181in isoform 2
Alternative sequenceVSP_056925182-342in isoform 2
Sequence conflict183-185in Ref. 1; BAB14289

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK022878
EMBL· GenBank· DDBJ
BAB14289.1
EMBL· GenBank· DDBJ
mRNA
AK293198
EMBL· GenBank· DDBJ
BAG56738.1
EMBL· GenBank· DDBJ
mRNA
AL160314
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC024779
EMBL· GenBank· DDBJ
AAH24779.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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