Q8TB40 · ABHD4_HUMAN
- Protein(Lyso)-N-acylphosphatidylethanolamine lipase
- GeneABHD4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids342 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.
Catalytic activity
- H2O + N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- H2O + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine = a fatty acid + H+ + N,1-diacyl-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- H2O + N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-hexadecanoyl-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- H2O + N-octadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-octadecanoyl-sn-glycero-3-phospho-ethanolamineThis reaction proceeds in the forward direction.
- H2O + N-eicosanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-eicosanoyl-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- H2O + N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine = (9Z)-octadecenoate + H+ + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
- 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H2O = (9Z)-octadecenoate + 1-octadecanoyl-2-hydroxy-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H+This reaction proceeds in the forward direction.
- 1-O-(1Z-octadecenoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = (9Z)-octadecenoate + 1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H+This reaction proceeds in the forward direction.
- H2O + N,1-diacyl-sn-glycero-3-phosphoethanolamine = a fatty acid + H+ + N-acyl-sn-glycero-3-phosphoethanolamineThis reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | lipid droplet | |
Cellular Component | mitochondrion | |
Molecular Function | hydrolase activity | |
Molecular Function | lysophosphatidic acid acyltransferase activity | |
Molecular Function | lysophospholipase activity | |
Biological Process | lipid catabolic process | |
Biological Process | lipid homeostasis | |
Biological Process | N-acylphosphatidylethanolamine metabolic process | |
Biological Process | phosphatidic acid biosynthetic process | |
Biological Process | phosphatidylethanolamine acyl-chain remodeling |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended name(Lyso)-N-acylphosphatidylethanolamine lipase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ8TB40
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 367 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000080864 | 1-342 | (Lyso)-N-acylphosphatidylethanolamine lipase | |||
Sequence: MADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVAGPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD |
Proteomic databases
PTM databases
Expression
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8TB40 | AURKAIP1 Q9NWT8 | 3 | EBI-7131019, EBI-448665 | |
BINARY | Q8TB40 | BNIP2 Q12982 | 3 | EBI-7131019, EBI-752094 | |
BINARY | Q8TB40 | CMTM5 Q96DZ9-2 | 3 | EBI-7131019, EBI-11522780 | |
BINARY | Q8TB40 | COL4A5 P29400-2 | 3 | EBI-7131019, EBI-12211159 | |
BINARY | Q8TB40 | DERL1 Q9BUN8 | 3 | EBI-7131019, EBI-398977 | |
BINARY | Q8TB40 | DGAT2L6 Q6ZPD8 | 3 | EBI-7131019, EBI-12831978 | |
BINARY | Q8TB40 | HTATIP2 Q9BUP3-3 | 3 | EBI-7131019, EBI-12937691 | |
BINARY | Q8TB40 | IGFBP5 P24593 | 3 | EBI-7131019, EBI-720480 | |
BINARY | Q8TB40 | INSIG2 Q9Y5U4 | 3 | EBI-7131019, EBI-8503746 | |
BINARY | Q8TB40 | PLIN3 O60664 | 3 | EBI-7131019, EBI-725795 | |
BINARY | Q8TB40 | RABAC1 Q9UI14 | 3 | EBI-7131019, EBI-712367 | |
BINARY | Q8TB40 | REEP5 Q00765 | 3 | EBI-7131019, EBI-1549827 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 70-201 | AB hydrolase-1 | ||||
Sequence: PLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRS |
Sequence similarities
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8TB40-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length342
- Mass (Da)38,794
- Last updated2002-06-01 v1
- Checksum867EA9E0092559CB
Q8TB40-2
- Name2
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, sequence conflict.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK022878 EMBL· GenBank· DDBJ | BAB14289.1 EMBL· GenBank· DDBJ | mRNA | ||
AK293198 EMBL· GenBank· DDBJ | BAG56738.1 EMBL· GenBank· DDBJ | mRNA | ||
AL160314 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC024779 EMBL· GenBank· DDBJ | AAH24779.1 EMBL· GenBank· DDBJ | mRNA |