Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

Q8T7E0 · ENPL_LEIDO

  • Protein
    Endoplasmin homolog
  • Gene
    LPG3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity).
Required for the synthesis of lipophosphoglycan (LPG), a cell surface glycoconjugate (PubMed:12198148).
Necessary for the attachment of the galactosyl residue to the mannose within the phosphoglycan repeats of the nascent LPG chain (PubMed:12198148).
Also required for addition of phosphoglycan to acid phosphatase (PubMed:12198148).
Not required for normal growth (PubMed:12198148).
Has ATPase activity (By similarity).
Binds heparin with micromolar affinity which may facilitate infection of host cells (By similarity).

Features

Showing features for binding site, site.

1771100200300400500600700
Type
IDPosition(s)Description
Binding site63ATP (UniProtKB | ChEBI)
Binding site109ATP (UniProtKB | ChEBI)
Binding site160ATP (UniProtKB | ChEBI)
Site405Important for ATP hydrolysis

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentendoplasmic reticulum
Cellular Componentperinuclear region of cytoplasm
Cellular Componentplasma membrane
Cellular Componentprotein-containing complex
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent protein folding chaperone
Molecular Functionheparin binding
Molecular Functionunfolded protein binding
Biological Processcellular response to heat
Biological Processprotein stabilization

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Endoplasmin homolog
  • Alternative names
    • Glucose-regulated protein 94
    • Lipophosphoglycan biosynthetic protein 3

Gene names

    • Name
      LPG3
    • Synonyms
      GRP94

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • MHOM/SD/62/1S
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leishmania

Accessions

  • Primary accession
    Q8T7E0

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Defective lipophosphoglycan synthesis and reduced levels of the surface glycoproteins gp46 and gp63.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis159In OB1; defective lipophosphoglycan (LPG) synthesis with production of a truncated LPG form lacking phosphoglycan repeats; defective addition of phosphoglycan to acid phosphatase.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

Type
IDPosition(s)Description
Signal1-24
ChainPRO_500431633425-771Endoplasmin homolog
Glycosylation63N-linked (GlcNAc...) asparagine
Glycosylation306N-linked (GlcNAc...) asparagine
Glycosylation402N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

Not induced by stress conditions such as heat, glucose deprivation or treatment with the glycosylation inhibitor tunicamycin.

Developmental stage

Expressed throughout the life cycle with lower expression in stationary phase promastigotes and higher levels in amastigotes.

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Features

Showing features for region, motif.

Type
IDPosition(s)Description
Region254-282Disordered
Region727-771Disordered
Motif768-771Prevents secretion from ER

Sequence similarities

Belongs to the heat shock protein 90 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    771
  • Mass (Da)
    86,938
  • Last updated
    2002-06-01 v1
  • MD5 Checksum
    728CEB47D5BAB251D52D6AC8D86D47D2
MANSSLLRVVLVALLLLGSVTVSAGDGRGTPIAFQAEVSKMLDILVNSLYTNRAVFLRELISNGSDALDKIRVLYLTSPKEPLTKDGEAPTMDLRISFDKEKSELILRDGGVGMTKEELAKHLGSLGTSGTKHFLEKLQEGVGAVGGDQNNLIGQFGVGFYSVFLVGDRVRVASKSDDSDEQYVWESKGDGQYFLYPDPRGNTLGRGTEITIELKPDAEQFLSAETIKKTIHQYSEFINFPIYVQEEVEVASTAATPESAAEERSLDEGAVEEDPDKEGDTQGVVKEKRWTLVNENRPIWTRPIGNVTEEEYHKFYKAFSGDYRDPLYFNHFKVEGEVDFDSILFVPTTVDPASFSDDNAVPNTNIKLYVRRVFITDEFRDLLPRYLNFVKGIVDSNDLPLNVSREVLQESRILRVIKKKLVRKTLSMFADIAAQDEAIANGKQVENPAPSGHTHLKKPAYTKFWELYGKHLRLGVMLDSNNRNRLTKLFRYKSSRSESEYISLQTYVDRMKKGQKGIYYLSGDSVARIKKSPVLEDAVNHDVEVIFMTDAIDEYVVSQLTDFAGKKLINLAKEGVQFEESDARQRVVDRKRKEKYDSFFTHLRALFGYSEVRKVILTKRMTNEAFIVSSSENQITARLASIMRGQSMSLANQQLTAERVLEVNYRHPLVDEMFKRFTVDEDDEVATDIAWVLYDTANLQAEFPVADVAAYSKRINRLLRSSVDLSADDSLLPPDDAEYTVSDTETEEEEEQPKVDTNAHEEAETDGEGDL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF369892
EMBL· GenBank· DDBJ
AAM00390.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help