Q8T7E0 · ENPL_LEIDO
- ProteinEndoplasmin homolog
- GeneLPG3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids771 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity).
Required for the synthesis of lipophosphoglycan (LPG), a cell surface glycoconjugate (PubMed:12198148).
Necessary for the attachment of the galactosyl residue to the mannose within the phosphoglycan repeats of the nascent LPG chain (PubMed:12198148).
Also required for addition of phosphoglycan to acid phosphatase (PubMed:12198148).
Not required for normal growth (PubMed:12198148).
Has ATPase activity (By similarity).
Binds heparin with micromolar affinity which may facilitate infection of host cells (By similarity).
Required for the synthesis of lipophosphoglycan (LPG), a cell surface glycoconjugate (PubMed:12198148).
Necessary for the attachment of the galactosyl residue to the mannose within the phosphoglycan repeats of the nascent LPG chain (PubMed:12198148).
Also required for addition of phosphoglycan to acid phosphatase (PubMed:12198148).
Not required for normal growth (PubMed:12198148).
Has ATPase activity (By similarity).
Binds heparin with micromolar affinity which may facilitate infection of host cells (By similarity).
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 63 | ATP (UniProtKB | ChEBI) | |||
Binding site | 109 | ATP (UniProtKB | ChEBI) | |||
Binding site | 160 | ATP (UniProtKB | ChEBI) | |||
Site | 405 | Important for ATP hydrolysis | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | perinuclear region of cytoplasm | |
Cellular Component | plasma membrane | |
Cellular Component | protein-containing complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent protein folding chaperone | |
Molecular Function | heparin binding | |
Molecular Function | unfolded protein binding | |
Biological Process | cellular response to heat | |
Biological Process | protein stabilization |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameEndoplasmin homolog
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Leishmaniinae > Leishmania
Accessions
- Primary accessionQ8T7E0
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Defective lipophosphoglycan synthesis and reduced levels of the surface glycoproteins gp46 and gp63.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 159 | In OB1; defective lipophosphoglycan (LPG) synthesis with production of a truncated LPG form lacking phosphoglycan repeats; defective addition of phosphoglycan to acid phosphatase. | |||
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-24 | ||||
Chain | PRO_5004316334 | 25-771 | Endoplasmin homolog | ||
Glycosylation | 63 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 306 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 402 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
PTM databases
Expression
Induction
Not induced by stress conditions such as heat, glucose deprivation or treatment with the glycosylation inhibitor tunicamycin.
Developmental stage
Expressed throughout the life cycle with lower expression in stationary phase promastigotes and higher levels in amastigotes.
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 254-282 | Disordered | |||
Region | 727-771 | Disordered | |||
Motif | 768-771 | Prevents secretion from ER | |||
Sequence similarities
Belongs to the heat shock protein 90 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length771
- Mass (Da)86,938
- Last updated2002-06-01 v1
- MD5 Checksum728CEB47D5BAB251D52D6AC8D86D47D2
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF369892 EMBL· GenBank· DDBJ | AAM00390.1 EMBL· GenBank· DDBJ | Genomic DNA |