Q8T2T7 · TOP3_DICDI
- ProteinDNA topoisomerase 3
- Genetop3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids828 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 361 | O-(5'-phospho-DNA)-tyrosine intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | RecQ family helicase-topoisomerase III complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
Biological Process | DNA recombination | |
Biological Process | DNA repair | |
Biological Process | DNA topological change |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA topoisomerase 3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Amoebozoa > Evosea > Eumycetozoa > Dictyostelia > Dictyosteliales > Dictyosteliaceae > Dictyostelium
Accessions
- Primary accessionQ8T2T7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000330480 | 1-828 | DNA topoisomerase 3 | ||
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-149 | Toprim | |||
Domain | 167-617 | Topo IA-type catalytic | |||
Region | 763-828 | Disordered | |||
Sequence similarities
Belongs to the type IA topoisomerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length828
- Mass (Da)94,639
- Last updated2002-06-01 v1
- MD5 ChecksumDD1B086DA95DBA250BA61EC79403A653
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFI02000013 EMBL· GenBank· DDBJ | EAL69909.2 EMBL· GenBank· DDBJ | Genomic DNA |