Q8SVN8 · NTE1_ENCCU
- ProteinLysophospholipase NTE1
- GeneNTE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids905 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H+ + sn-glycerol 3-phosphocholine
Activity regulation
Inhibited by organophosphorus esters.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 198-319 | a nucleoside 3',5'-cyclic phosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: FLSFLNEKLLLNLDDGKEILRRDIEARLIGRGETIKDSEESMDYIMYVVSGEILVLAGESAFVFGKGSVFGYFSLFFDLYSSIKIQAREDSSVLLCSSSVLLKFGLDEKKFDHSMLLDIDES | ||||||
Binding site | 310-428 | a nucleoside 3',5'-cyclic phosphate 2 (UniProtKB | ChEBI) | ||||
Sequence: HSMLLDIDESLHIIDESAEWMRLLPGDMIAQKGDASKEIFYIGAGSVKSASRESSSGTVIGGKECIFGESWNESSIATRITDVVRIPSLLVDYFLERDKSFFKKYTKRLFESGNKANGK | ||||||
Active site | 643 | Nucleophile | ||||
Sequence: S | ||||||
Active site | 761 | Proton acceptor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | lysophospholipase activity | |
Biological Process | lipid catabolic process | |
Biological Process | phosphatidylcholine metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLysophospholipase NTE1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Fungi incertae sedis > Microsporidia > Unikaryonidae > Encephalitozoon
Accessions
- Primary accessionQ8SVN8
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass type I membrane protein
Features
Showing features for transmembrane, topological domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | ||||
Sequence: MVSLSLLIAIIVVTGLLAGFR | ||||||
Topological domain | 22-905 | Cytoplasmic | ||||
Sequence: HYGSSRCRTVPFKIKSRSIEKTASTRLLGMSLEDAMKRYPLFNFYPGQSIREILSSWERVTYGNGETIAAPGFILQGLVEIWYREHLVCVKEKGSFLNGSMELLGYSTRGTKKARGSVEVLLIDASLVRDNIWYILFSMLRKSCIEIACRYFELESKLVEKETMKIKDDGQGHLDLFLSFLNEKLLLNLDDGKEILRRDIEARLIGRGETIKDSEESMDYIMYVVSGEILVLAGESAFVFGKGSVFGYFSLFFDLYSSIKIQAREDSSVLLCSSSVLLKFGLDEKKFDHSMLLDIDESLHIIDESAEWMRLLPGDMIAQKGDASKEIFYIGAGSVKSASRESSSGTVIGGKECIFGESWNESSIATRITDVVRIPSLLVDYFLERDKSFFKKYTKRLFESGNKANGKIVSIIPVGQYKDIESFSRKLKSAIGASSLLLSRRDVVEILARRAFDTTEEVRFMDYLTKMSRRYEIILIYVENEYSRLLRYLLNFSDVLLAVGTTFADIPEYNVYCRIEFVKIYEERRASDERSVKKSKKINHGPRYLESRKESFGQYDRVHHVLFPSKTMLFCSKDFQRLARSLLGKRIGLVLGGGGARGLAHIGVIQALEEEGIPIDCVGGTSMGAFIGALYAKECNNYHVFKQAKRFSRKMSNIWILLLDLTYPICSMFSGHAFNRSLHSIFGDGLIQDLWLEYFCITTNIVSYEEEVHRNGMVWRYVRASMGLCGYLPPICDNKKLLVDGGYLNNVPADVMMGMQVEKIITVDVGTVLENDHFDYGDTLSGFTILFNKFFGNKKFVTMEEIQYRLAYISTERKMKELEANGNIIMLRPDLGKYRTMDFKKFDEIVACGYQSTKNAIAKWKQEGTYDVLFFSLKKQTPKRRYSV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000295321 | 1-905 | Lysophospholipase NTE1 | |||
Sequence: MVSLSLLIAIIVVTGLLAGFRHYGSSRCRTVPFKIKSRSIEKTASTRLLGMSLEDAMKRYPLFNFYPGQSIREILSSWERVTYGNGETIAAPGFILQGLVEIWYREHLVCVKEKGSFLNGSMELLGYSTRGTKKARGSVEVLLIDASLVRDNIWYILFSMLRKSCIEIACRYFELESKLVEKETMKIKDDGQGHLDLFLSFLNEKLLLNLDDGKEILRRDIEARLIGRGETIKDSEESMDYIMYVVSGEILVLAGESAFVFGKGSVFGYFSLFFDLYSSIKIQAREDSSVLLCSSSVLLKFGLDEKKFDHSMLLDIDESLHIIDESAEWMRLLPGDMIAQKGDASKEIFYIGAGSVKSASRESSSGTVIGGKECIFGESWNESSIATRITDVVRIPSLLVDYFLERDKSFFKKYTKRLFESGNKANGKIVSIIPVGQYKDIESFSRKLKSAIGASSLLLSRRDVVEILARRAFDTTEEVRFMDYLTKMSRRYEIILIYVENEYSRLLRYLLNFSDVLLAVGTTFADIPEYNVYCRIEFVKIYEERRASDERSVKKSKKINHGPRYLESRKESFGQYDRVHHVLFPSKTMLFCSKDFQRLARSLLGKRIGLVLGGGGARGLAHIGVIQALEEEGIPIDCVGGTSMGAFIGALYAKECNNYHVFKQAKRFSRKMSNIWILLLDLTYPICSMFSGHAFNRSLHSIFGDGLIQDLWLEYFCITTNIVSYEEEVHRNGMVWRYVRASMGLCGYLPPICDNKKLLVDGGYLNNVPADVMMGMQVEKIITVDVGTVLENDHFDYGDTLSGFTILFNKFFGNKKFVTMEEIQYRLAYISTERKMKELEANGNIIMLRPDLGKYRTMDFKKFDEIVACGYQSTKNAIAKWKQEGTYDVLFFSLKKQTPKRRYSV |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 610-774 | PNPLA | ||||
Sequence: LVLGGGGARGLAHIGVIQALEEEGIPIDCVGGTSMGAFIGALYAKECNNYHVFKQAKRFSRKMSNIWILLLDLTYPICSMFSGHAFNRSLHSIFGDGLIQDLWLEYFCITTNIVSYEEEVHRNGMVWRYVRASMGLCGYLPPICDNKKLLVDGGYLNNVPADVMM | ||||||
Motif | 614-619 | GXGXXG | ||||
Sequence: GGGARG | ||||||
Motif | 641-645 | GXSXG | ||||
Sequence: GTSMG | ||||||
Motif | 761-763 | DGA/G | ||||
Sequence: DGG |
Sequence similarities
Belongs to the NTE family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length905
- Mass (Da)103,088
- Last updated2002-06-01 v1
- Checksum94B9D7775EA8E92C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL590445 EMBL· GenBank· DDBJ | CAD26524.1 EMBL· GenBank· DDBJ | Genomic DNA |