Q8SRB8 · NTH_ENCCU
- ProteinEndonuclease III homolog
- GeneNTH1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids238 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 149 | Nucleophile; for N-glycosylase activity | ||||
Sequence: K | ||||||
Site | 168 | Important for catalytic activity | ||||
Sequence: D | ||||||
Binding site | 217 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 224 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 227 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 233 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity | |
Biological Process | base-excision repair, AP site formation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEndonuclease III homolog
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Fungi incertae sedis > Microsporidia > Unikaryonidae > Encephalitozoon
Accessions
- Primary accessionQ8SRB8
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000382919 | 1-238 | Endonuclease III homolog | |||
Sequence: MGSASGEEREGPLGLYLEIKMQRKDIVSPVDTMGCSITPSCRTEEERRFHILVSLLLSSQTKDEVTYEAMARLRKLLPESAATDGEARGGLTIERVANSDVKHINECIKKVGFHNRKAANLKKIAEILREKGLPREMKDLISLPGIGNKMALLYMSHACNRTVGISVDTHVHRISNRIGLVRTRDVESTRRELERVVPRKEWKTINNILVGFGQTICVAKRPRCEECCIRGRCPSSLF |
Expression
Developmental stage
Expressed in late sporogonial stages.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 129-155 | HhH | ||||
Sequence: REKGLPREMKDLISLPGIGNKMALLYM |
Sequence similarities
Belongs to the Nth/MutY family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length238
- Mass (Da)26,755
- Last updated2002-06-01 v1
- Checksum3D9D7B774187F35B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL590448 EMBL· GenBank· DDBJ | CAD26394.1 EMBL· GenBank· DDBJ | Genomic DNA |