Q8RXS6 · INO80_ARATH
- ProteinChromatin-remodeling ATPase INO80
- GeneINO80
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1507 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (By similarity).
Binds DNA (By similarity).
As part of the INO80 complex, remodels chromatin by shifting nucleosomes (By similarity).
The INO80 complex controls ethylene-induced H2A.Z eviction dynamics (PubMed:31418686).
Positive regulator of homologous recombination, but not an essential component of homologous recombination (PubMed:15525519).
Not involved in the illegitimate repair pathway (PubMed:15525519).
Binds DNA (By similarity).
As part of the INO80 complex, remodels chromatin by shifting nucleosomes (By similarity).
The INO80 complex controls ethylene-induced H2A.Z eviction dynamics (PubMed:31418686).
Positive regulator of homologous recombination, but not an essential component of homologous recombination (PubMed:15525519).
Not involved in the illegitimate repair pathway (PubMed:15525519).
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 611-618 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Ino80 complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | DNA binding | |
Molecular Function | histone binding | |
Biological Process | chromatin remodeling | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameChromatin-remodeling ATPase INO80
- EC number
- Short namesAtINO80
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8RXS6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Decreased homologous recombination frequency, but unchanged sensitivity to genotoxic agents and efficiency of T-DNA integration (PubMed:15525519).
Differential expression of several genes (PubMed:31418686).
Compromised ethylene-induced H2A.Z eviction dynamics (PubMed:31418686).
The double mutant ref6-1 ino80-1 is insensitive to ethylene (ET) and exhibits reduced levels of EIN2 associated with a shift of the chromatin landscape to a repressive state at its locus (e.g. H3K27me3 and H2A.Z) (PubMed:31418686).
Differential expression of several genes (PubMed:31418686).
Compromised ethylene-induced H2A.Z eviction dynamics (PubMed:31418686).
The double mutant ref6-1 ino80-1 is insensitive to ethylene (ET) and exhibits reduced levels of EIN2 associated with a shift of the chromatin landscape to a repressive state at its locus (e.g. H3K27me3 and H2A.Z) (PubMed:31418686).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 80 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000375865 | 1-1507 | Chromatin-remodeling ATPase INO80 | ||
Proteomic databases
PTM databases
Expression
Induction
Not induced by methyl methanesulfonate (MMS) treatment.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 30-82 | Disordered | |||
Compositional bias | 40-64 | Polar residues | |||
Domain | 350-475 | DBINO | |||
Region | 428-452 | Disordered | |||
Domain | 598-769 | Helicase ATP-binding | |||
Domain | 1210-1360 | Helicase C-terminal | |||
Region | 1415-1507 | Disordered | |||
Compositional bias | 1420-1442 | Basic and acidic residues | |||
Compositional bias | 1454-1480 | Basic and acidic residues | |||
Compositional bias | 1481-1507 | Polar residues | |||
Domain
The DBINO region is involved in binding to DNA.
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q8RXS6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,507
- Mass (Da)172,172
- Last updated2009-05-26 v2
- ChecksumB64F7CCF96452E6B
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4J277 | F4J277_ARATH | INO80 | 1540 | ||
A0A1I9LT22 | A0A1I9LT22_ARATH | INO80 | 1252 |
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 40-64 | Polar residues | |||
Compositional bias | 1420-1442 | Basic and acidic residues | |||
Compositional bias | 1454-1480 | Basic and acidic residues | |||
Compositional bias | 1481-1507 | Polar residues | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL137080 EMBL· GenBank· DDBJ | CAB68136.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE79637.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | ANM65731.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY080695 EMBL· GenBank· DDBJ | AAL86315.1 EMBL· GenBank· DDBJ | mRNA |