Q8RXD3 · AIP2_ARATH
- ProteinE3 ubiquitin-protein ligase AIP2
- GeneAIP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids310 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E3 ubiquitin-protein ligase that acts as a negative regulator of abscisic acid (ABA) signaling. Mediates ubiquitination and subsequent proteasomal degradation of the transcription factor ABI3.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | abscisic acid-activated signaling pathway | |
Biological Process | negative regulation of abscisic acid-activated signaling pathway | |
Biological Process | protein ubiquitination | |
Biological Process | response to abscisic acid |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase AIP2
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8RXD3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Hypersensitivity to abscisic acid (ABA).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 230 | Loss of E3 ubiquitin ligase activity. | ||||
Sequence: C → S | ||||||
Mutagenesis | 231 | Loss of E3 ubiquitin ligase activity. | ||||
Sequence: C → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 25 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000395844 | 1-310 | E3 ubiquitin-protein ligase AIP2 | |||
Sequence: MDASSSPSPSEESLKLELDDLQKQLNKKLRFEASVCSIHNLLRDHYSSSSPSLRKQFYIVVSRVATVLKTRYTATGFWVAGLSLFEEAERLVSDASEKKHLKSCVAQAKEQLSEVDNQPTESSQGYLFEGHLTVDREPPQPQWLVQQNLMSAFASIVGGESSNGPTENTIGETANLMQELINGLDMIIPDILDDGGPPRAPPASKEVVEKLPVIIFTEELLKKFGAEAECCICKENLVIGDKMQELPCKHTFHPPCLKPWLDEHNSCPICRHELPTDDQKYENWKEREKEAEEERKGAENAVRGGEYMYV |
Post-translational modification
Auto-ubiquitinated.
Keywords
- PTM
Proteomic databases
Expression
Tissue specificity
Highly expressed in leaves and at lower levels in flowers and seeds.
Gene expression databases
Interaction
Subunit
Interacts with ABI3 (via C-terminus).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8RXD3 | ABI3 Q01593 | 3 | EBI-2312425, EBI-1578892 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for zinc finger, coiled coil, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 230-271 | RING-type; atypical | ||||
Sequence: CCICKENLVIGDKMQELPCKHTFHPPCLKPWLDEHNSCPICR | ||||||
Coiled coil | 276-306 | |||||
Sequence: TDDQKYENWKEREKEAEEERKGAENAVRGGE | ||||||
Compositional bias | 285-301 | Basic and acidic residues | ||||
Sequence: KEREKEAEEERKGAENA | ||||||
Region | 285-310 | Disordered | ||||
Sequence: KEREKEAEEERKGAENAVRGGEYMYV |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length310
- Mass (Da)34,807
- Last updated2002-06-01 v1
- Checksum47D3C96AF08D034E
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 277 | in Ref. 1; CAB75509 | ||||
Sequence: D → A | ||||||
Compositional bias | 285-301 | Basic and acidic residues | ||||
Sequence: KEREKEAEEERKGAENA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ251087 EMBL· GenBank· DDBJ | CAB75509.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ059130 EMBL· GenBank· DDBJ | AAY57616.1 EMBL· GenBank· DDBJ | mRNA | ||
AF296834 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002688 EMBL· GenBank· DDBJ | AED92903.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY081329 EMBL· GenBank· DDBJ | AAL91218.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000238 EMBL· GenBank· DDBJ | AAN15557.1 EMBL· GenBank· DDBJ | mRNA |