Q8RWV0 · TKTC1_ARATH

Function

function

Catalyzes the reversible transfer of a two-carbon ketol group from fructose-6-phosphate or sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate, respectively (By similarity).
Could act as a stress sensor involved in adaptation process

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Co2+ (UniProtKB | Rhea| CHEBI:48828 )

Note: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.
thiamine diphosphate (UniProtKB | Rhea| CHEBI:58937 )

Note: Binds 1 thiamine pyrophosphate per subunit.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site103substrate
Site103Important for catalytic activity
Binding site143thiamine diphosphate (UniProtKB | ChEBI)
Binding site192-194thiamine diphosphate (UniProtKB | ChEBI)
Binding site233Mg2+ (UniProtKB | ChEBI)
Binding site234thiamine diphosphate (UniProtKB | ChEBI)
Binding site263Mg2+ (UniProtKB | ChEBI)
Binding site263thiamine diphosphate (UniProtKB | ChEBI)
Binding site265Mg2+ (UniProtKB | ChEBI)
Binding site340substrate
Binding site340thiamine diphosphate (UniProtKB | ChEBI)
Site340Important for catalytic activity
Binding site434substrate
Binding site461substrate
Active site488Proton donor
Binding site488thiamine diphosphate (UniProtKB | ChEBI)
Binding site515thiamine diphosphate (UniProtKB | ChEBI)
Binding site539substrate
Binding site547substrate
Binding site598substrate

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast envelope
Cellular Componentchloroplast stroma
Cellular Componentcytosol
Cellular Componentplastid
Molecular Functionmetal ion binding
Molecular FunctionmRNA binding
Molecular Functiontransketolase activity
Biological Processreductive pentose-phosphate cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transketolase-1, chloroplastic
  • EC number
  • Short names
    TK

Gene names

    • Name
      TKL-1
    • ORF names
      T4C21_160
    • Ordered locus names
      At3g60750

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8RWV0
  • Secondary accessions
    • B9DGH8
    • Q84WI5
    • Q8LE99
    • Q944P9
    • Q9LZY8

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for transit peptide, modified residue, chain.

TypeIDPosition(s)Description
Transit peptide1-66Chloroplast
Modified residue67N-acetylalanine
ChainPRO_000042181767-741Transketolase-1, chloroplastic
Modified residue428Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Induction

Up-regulated by salt, sorbitol, and abscisic acid (ABA).

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region22-51Disordered
Compositional bias30-51Polar residues

Sequence similarities

Belongs to the transketolase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q8RWV0-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    741
  • Mass (Da)
    79,968
  • Last updated
    2002-06-01 v1
  • Checksum
    5886543FBA70E79C
MASTSSLALSQALLARAISHHGSDQRGSLPAFSGLKSTGSRASASSRRRIAQSMTKNRSLRPLVRAAAVETVEPTTDSSIVDKSVNSIRFLAIDAVEKAKSGHPGLPMGCAPMAHILYDEVMRYNPKNPYWFNRDRFVLSAGHGCMLLYALLHLAGYDSVQEEDLKQFRQWGSKTPGHPENFETPGIEVTTGPLGQGIANAVGLALAEKHLAARFNKPDAEVVDHYTYAILGDGCQMEGISNEACSLAGHWGLGKLIAFYDDNHISIDGDTEIAFTENVDQRFEALGWHVIWVKNGNTGYDEIRAAIKEAKTVTDKPTLIKVTTTIGYGSPNKANSYSVHGAALGEKEVEATRNNLGWPYEPFQVPDDVKSHWSRHTPEGATLESDWSAKFAAYEKKYPEEASELKSIITGELPAGWEKALPTYTPESPGDATRNLSQQCLNALAKVVPGFLGGSADLASSNMTLLKAFGDFQKATPEERNLRFGVREHGMGAICNGIALHSPGLIPYCATFFVFTDYMRGAMRISALSEAGVIYVMTHDSIGLGEDGPTHQPIEHIASFRAMPNTLMFRPADGNETAGAYKIAVTKRKTPSILALSRQKLPHLPGTSIEGVEKGGYTISDDSSGNKPDVILIGTGSELEIAAQAAEVLRKDGKTVRVVSFVCWELFDEQSDEYKESVLPSDVSARVSIEAASTFGWGKIVGGKGKSIGINSFGASAPAPLLYKEFGITVEAVVDAAKSFF

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4JBY2F4JBY2_ARATHTKL1740

Sequence caution

The sequence AAL11624.1 differs from that shown. Reason: Frameshift
The sequence AAN18173.1 differs from that shown. Reason: Frameshift
The sequence CAB82679.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias30-51Polar residues
Sequence conflict282in Ref. 4; AAM62766
Sequence conflict482in Ref. 3; AAO29950

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL162295
EMBL· GenBank· DDBJ
CAB82679.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002686
EMBL· GenBank· DDBJ
AEE80103.1
EMBL· GenBank· DDBJ
Genomic DNA
AF424631
EMBL· GenBank· DDBJ
AAL11624.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AY091094
EMBL· GenBank· DDBJ
AAM14045.1
EMBL· GenBank· DDBJ
mRNA
AY133860
EMBL· GenBank· DDBJ
AAM91794.1
EMBL· GenBank· DDBJ
mRNA
BT000604
EMBL· GenBank· DDBJ
AAN18173.1
EMBL· GenBank· DDBJ
mRNA Frameshift
BT003331
EMBL· GenBank· DDBJ
AAO29950.1
EMBL· GenBank· DDBJ
mRNA
AY085542
EMBL· GenBank· DDBJ
AAM62766.1
EMBL· GenBank· DDBJ
mRNA
AK317159
EMBL· GenBank· DDBJ
BAH19845.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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