Q8RWU6 · NHX6_ARATH
- ProteinSodium/hydrogen exchanger 6
- GeneNHX6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids535 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in trafficking to the vacuole (PubMed:21278129).
Required for cell proliferation and cell expansion, but not for cell differentiation (PubMed:21278129).
Acts in low affinity electroneutral exchange of protons for cations such as Na+ or K+ across membranes (By similarity).
May also exchange Li+ and Cs+ with a lower affinity (By similarity).
Required for cell proliferation and cell expansion, but not for cell differentiation (PubMed:21278129).
Acts in low affinity electroneutral exchange of protons for cations such as Na+ or K+ across membranes (By similarity).
May also exchange Li+ and Cs+ with a lower affinity (By similarity).
Catalytic activity
- Na+(in) + H+(out) = Na+(out) + H+(in)
- K+(in) + H+(out) = K+(out) + H+(in)
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endosome | |
Cellular Component | endosome membrane | |
Cellular Component | Golgi cisterna membrane | |
Molecular Function | sodium:proton antiporter activity | |
Biological Process | potassium ion transport | |
Biological Process | regulation of pH |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSodium/hydrogen exchanger 6
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8RWU6
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Multi-pass membrane protein
Golgi apparatus, Golgi stack membrane ; Multi-pass membrane protein
Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-24 | Cytoplasmic | ||||
Sequence: MSSELQISPAIHDPQGQEKQQQAA | ||||||
Transmembrane | 25-45 | Helical | ||||
Sequence: GVGILLQIMMLVLSFVLGHVL | ||||||
Topological domain | 46-49 | Lumenal | ||||
Sequence: RRHK | ||||||
Transmembrane | 50-70 | Helical | ||||
Sequence: FYYLPEASASLLIGLIVGGLA | ||||||
Topological domain | 71-87 | Cytoplasmic | ||||
Sequence: NISNTETSIRTWFNFHD | ||||||
Intramembrane | 88-108 | Helical | ||||
Sequence: EFFFLFLLPPIIFQSGFSLQP | ||||||
Topological domain | 109-126 | Cytoplasmic | ||||
Sequence: KPFFSNFGAIVTFSVLGT | ||||||
Transmembrane | 127-147 | Helical | ||||
Sequence: FVASMVTGLLVYLGGVMFLMY | ||||||
Topological domain | 148 | Lumenal | ||||
Sequence: R | ||||||
Transmembrane | 149-169 | Helical | ||||
Sequence: LPFVECLMFGSLISATDPVTV | ||||||
Topological domain | 170-179 | Cytoplasmic | ||||
Sequence: LSIFQELGSD | ||||||
Transmembrane | 180-200 | Helical | ||||
Sequence: VNLYALVFGESVLNDAMAISL | ||||||
Topological domain | 201-215 | Lumenal | ||||
Sequence: YRTMSLVRSHSSGQN | ||||||
Transmembrane | 216-236 | Helical | ||||
Sequence: FFMVIVRFLETFVGSMSAGVG | ||||||
Topological domain | 237-271 | Cytoplasmic | ||||
Sequence: VGFTSALLFKYAGLDVDNLQNLECCLFVLFPYFSY | ||||||
Transmembrane | 272-292 | Helical | ||||
Sequence: MLAEGLSLSGIVSILFTGIVM | ||||||
Topological domain | 293-306 | Lumenal | ||||
Sequence: KHYTYSNLSANSQR | ||||||
Transmembrane | 307-327 | Helical | ||||
Sequence: FVSAFFHLISSLAETFVFIYM | ||||||
Topological domain | 328-340 | Cytoplasmic | ||||
Sequence: GFDIAMEKHSWSH | ||||||
Transmembrane | 341-361 | Helical | ||||
Sequence: LGFIFFSILFIVIARAANVFG | ||||||
Topological domain | 362-382 | Lumenal | ||||
Sequence: CGYLVNLARPAHRKIPMTHQK | ||||||
Transmembrane | 383-402 | Helical | ||||
Sequence: ALWYSGLRGAMAFALALQSV | ||||||
Topological domain | 403-412 | Cytoplasmic | ||||
Sequence: HDLPEGHGQT | ||||||
Transmembrane | 413-433 | Helical | ||||
Sequence: IFTATTAIVVLTVLLIGGSTG | ||||||
Topological domain | 434-535 | Lumenal | ||||
Sequence: TMLEALEVVGDSHDTSLGDGFEVVNSRYMTSYDDEDTPPGSGFRTKLREFHKSAASFTELDRNYLTPFFTSNNGDYDDEGNMEQHHEERIPFTRRGNLNNRG |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype; due to redundancy with NHX5. Nhx5 and nhx6 double mutant has a slower development, is drastically smaller and is salt sensitive.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000052377 | 1-535 | Sodium/hydrogen exchanger 6 | |||
Sequence: MSSELQISPAIHDPQGQEKQQQAAGVGILLQIMMLVLSFVLGHVLRRHKFYYLPEASASLLIGLIVGGLANISNTETSIRTWFNFHDEFFFLFLLPPIIFQSGFSLQPKPFFSNFGAIVTFSVLGTFVASMVTGLLVYLGGVMFLMYRLPFVECLMFGSLISATDPVTVLSIFQELGSDVNLYALVFGESVLNDAMAISLYRTMSLVRSHSSGQNFFMVIVRFLETFVGSMSAGVGVGFTSALLFKYAGLDVDNLQNLECCLFVLFPYFSYMLAEGLSLSGIVSILFTGIVMKHYTYSNLSANSQRFVSAFFHLISSLAETFVFIYMGFDIAMEKHSWSHLGFIFFSILFIVIARAANVFGCGYLVNLARPAHRKIPMTHQKALWYSGLRGAMAFALALQSVHDLPEGHGQTIFTATTAIVVLTVLLIGGSTGTMLEALEVVGDSHDTSLGDGFEVVNSRYMTSYDDEDTPPGSGFRTKLREFHKSAASFTELDRNYLTPFFTSNNGDYDDEGNMEQHHEERIPFTRRGNLNNRG | ||||||
Glycosylation | 299 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 470 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in roots, leaves, stems, flowers and siliques. Detected at very low levels in roots and shoots.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 508-535 | Disordered | ||||
Sequence: DYDDEGNMEQHHEERIPFTRRGNLNNRG | ||||||
Compositional bias | 511-527 | Basic and acidic residues | ||||
Sequence: DEGNMEQHHEERIPFTR |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8RWU6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length535
- Mass (Da)59,314
- Last updated2011-05-03 v3
- Checksum101E72E26D409080
Q8RWU6-2
- Name2
- NoteMay be due to an intron retention.
- Differences from canonical
- 520-535: EERIPFTRRGNLNNRG → GNNIIL
Sequence caution
Features
Showing features for sequence conflict, compositional bias, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 243 | in Ref. 2; AAF68127 | ||||
Sequence: L → H | ||||||
Compositional bias | 511-527 | Basic and acidic residues | ||||
Sequence: DEGNMEQHHEERIPFTR | ||||||
Alternative sequence | VSP_016703 | 520-535 | in isoform 2 | |||
Sequence: EERIPFTRRGNLNNRG → GNNIIL |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF490590 EMBL· GenBank· DDBJ | AAM08407.1 EMBL· GenBank· DDBJ | mRNA | ||
AC010793 EMBL· GenBank· DDBJ | AAF68127.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE36272.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY091100 EMBL· GenBank· DDBJ | AAM14051.1 EMBL· GenBank· DDBJ | mRNA | ||
AY133748 EMBL· GenBank· DDBJ | AAM91682.1 EMBL· GenBank· DDBJ | mRNA |