Q8RWL6 · STY17_ARATH

Function

function

Serine/threonine protein kinase that specifically phosphorylates chloroplast precursor proteins in the cytosol within the cleavable presequences (transit peptides). May be part of a cytosolic regulatory network involved in chloroplast protein import. Does not phosphorylate mitochondrion precursor proteins. Specific for ATP and does not utilize other NTPs (PubMed:16429265, PubMed:17090544).
Plays a role in chloroplast biogenesis and differentiation in cotyledons, possibly through phosphorylation of chloroplast preproteins (PubMed:21799034).

Miscellaneous

Plants silencing STY17 does not show visible phenotype under normal growth conditions, but plants silencing STY17 in the double mutant sty8 and sty46 background show more severe retarded growth compared to the double mutant itself.

Catalytic activity

Activity regulation

Activated by autophosphorylation at Thr-445.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
14 μMATP

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site298-306ATP (UniProtKB | ChEBI)
Binding site319ATP (UniProtKB | ChEBI)
Active site413Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Molecular Functionprotein serine/threonine/tyrosine kinase activity
Biological Processchloroplast organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase STY17
  • EC number
  • Alternative names
    • Serine/threonine/tyrosine-protein kinase 17

Gene names

    • Name
      STY17
    • ORF names
      F8D20.290
    • Ordered locus names
      At4g35780

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q8RWL6
  • Secondary accessions
    • O81808

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis445Loss of autophosphorylation and enzyme activation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 21 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004339991-570Serine/threonine-protein kinase STY17
Modified residue441Phosphoserine
Modified residue445Phosphothreonine

Post-translational modification

Autophosphorylated on serine and threonine residues. Autophosphorylated at Thr-445.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region112-145Disordered
Domain180-260ACT
Domain292-545Protein kinase

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    570
  • Mass (Da)
    64,696
  • Last updated
    2002-06-01 v1
  • Checksum
    D0961D218BE49344
MAIKEETEESCGSRAVVASITKESPRQHRMKLEVYGEVLQRIQESNYEEANFPGFDDLLWLHFNRLPARYALDVNVERAEDVLTHQRLLKLAEDPATRPVFEVRCVQVSPTLNGNSGDVDPSDPAVNEDAQSSYNSRSLAPPTFGSSPNFEALTQAYKDHAQDDDSAVNAQLPNSRPMHEITFSTIDRPKLLSQLTSMLGELGLNIQEAHAFSTADGFSLDVFVVDGWSQEETEGLKDALKKEIRKFKDQPCSKQKSITFFEHDKSTNELLPACVEIPTDGTDEWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRKVRHKNVVQFIGACTRSPNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMNYLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESGVMTAETGTYRWMAPEVIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLTELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDDERHKDKHGGYFSGLKKGHR

Sequence caution

The sequence CAA20048.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB81487.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL031135
EMBL· GenBank· DDBJ
CAA20048.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161588
EMBL· GenBank· DDBJ
CAB81487.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE86563.1
EMBL· GenBank· DDBJ
Genomic DNA
AY093017
EMBL· GenBank· DDBJ
AAM13016.1
EMBL· GenBank· DDBJ
mRNA
AY128938
EMBL· GenBank· DDBJ
AAM91338.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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