Q8RWK8 · COIL_ARATH
- ProteinCoilin
- GeneCOIL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids608 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Probable component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs (Probable). Required for CBs formation (PubMed:16624863).
Binds snRNAs and non-specific artificial RNA via the N-terminal part of the NOD domain and via the NLS2 region (212-282) of the IDD domain (PubMed:23320094).
The two sites are able to function independently and provide effective RNA-binding in a non-cooperative manner (PubMed:23320094).
Binds snRNAs and non-specific artificial RNA via the N-terminal part of the NOD domain and via the NLS2 region (212-282) of the IDD domain (PubMed:23320094).
The two sites are able to function independently and provide effective RNA-binding in a non-cooperative manner (PubMed:23320094).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Cajal body | |
Molecular Function | U1 snRNA binding | |
Molecular Function | U2 snRNA binding | |
Biological Process | RNA processing |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameCoilin
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8RWK8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No visible phenotype, but loss of Cajal bodies.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 15 | In NOD-RK1; Decreased RNA-binding; when associated with A-16; A-20; A-23 and A-24. In NOD-RK1-2; Loss of RNA-binding; when associated with A-16; A-20; A-23; A-24; A-36; A-37; A-39 and A-40. | ||||
Sequence: R → A | ||||||
Mutagenesis | 16 | In NOD-RK1; Decreased RNA-binding; when associated with A-15; A-20; A-23 and A-24. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-20; A-23; A-24; A-36; A-37; A-39 and A-40. | ||||
Sequence: R → A | ||||||
Mutagenesis | 20 | In NOD-RK1; Decreased RNA-binding; when associated with A-15; A-16; A-23 and A-24. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-23; A-24; A-36; A-37; A-39 and A-40. | ||||
Sequence: K → A | ||||||
Mutagenesis | 23 | In NOD-RK1; Decreased RNA-binding; when associated with A-15; A-16; A-20 and A-24. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-24; A-36; A-37; A-39 and A-40. | ||||
Sequence: K → A | ||||||
Mutagenesis | 24 | In NOD-RK1; Decreased RNA-binding; when associated with A-15; A-16; A-20 and A-23. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-23; A-36; A-37; A-39 and A-40. | ||||
Sequence: K → A | ||||||
Mutagenesis | 36 | In NOD-RK2; No effect on RNA-binding; when associated with A-37; A39 and A-40. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-23; A-24; A-37; A-39 and A-40. | ||||
Sequence: R → A | ||||||
Mutagenesis | 37 | In NOD-RK2; No effect on RNA-binding; when associated with A-36; A-39 and A-40. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-23; A-24; A-36; A-39 and A-40. | ||||
Sequence: K → A | ||||||
Mutagenesis | 39 | In NOD-RK2; No effect on RNA-binding; when associated with A-36; A-37 and A-40. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-23; A-24; A-36; A-37 and A-40. | ||||
Sequence: H → A | ||||||
Mutagenesis | 40 | In NOD-RK2; No effect on RNA-binding; when associated with A-36; A-37 and A-39. In NOD-RK1-2; Loss of RNA-binding; when associated with A-15; A-16; A-20; A-23; A-24; A-36; A-37 and A-39. | ||||
Sequence: R → A | ||||||
Mutagenesis | 106 | In ncb-3; Smaller Cajal bodies. | ||||
Sequence: E → K |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 56 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434410 | 1-608 | Coilin | |||
Sequence: MEEEKVRVRLVFEDRRILSKYQKKQGLTRSWVVLNRKCHRTISEFSDHIFHTFSLCEACPHGLSLSMEGFVLPPFESSCVLKDKDIVCVKKKKESLLEIVGEDSDENVYNAIEVEERPQIRPGEMLLANEEFQKETGGYESESEEDELEEEAEEFVPEKKASKKRKTSSKNQSTKRKKCKLDTTEESPDERENTAVVSNVVKKKKKKKSLDVQSANNDEQNNDSTKPMTKSKRSSQQEESKEHNDLCQLSAETKKTPSRSARRKKAKRQWLREKTKLEKEELLQTQLVVAPSQKPVITIDHQATKEKHCETLENQQAEEVSDGFGDEVVPVEVRPGHIRFKPLAGTDEASLDSEPLVENVLWNGNMTKKKGQKWGTEKSGFSKRYAQDFNEDATTQPAEAETLANCPIDYEQLVAYTGSVKKGDVIAYRLIELTSSWTPEVSSFRVGKISYYDPDSKMVTLMPVQEFPIEKKTEEDDDFCMQPDTSLYKEDGSLEIEFSALLDVRSVKTSSSDSAEVAKSALPEPDQSAKKPKLSANKELQTPAKENGEVSPWEELSEALSAKKAALSQANNGWNKKGSSSGGSWSYKALRGSAMGPVMNYLRSQKEI | ||||||
Modified residue | 187 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, motif, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 134-272 | Disordered | ||||
Sequence: KETGGYESESEEDELEEEAEEFVPEKKASKKRKTSSKNQSTKRKKCKLDTTEESPDERENTAVVSNVVKKKKKKKSLDVQSANNDEQNNDSTKPMTKSKRSSQQEESKEHNDLCQLSAETKKTPSRSARRKKAKRQWLR | ||||||
Motif | 163-170 | Nuclear localization signal 1 | ||||
Sequence: KKRKTSSK | ||||||
Compositional bias | 175-199 | Basic and acidic residues | ||||
Sequence: KRKKCKLDTTEESPDERENTAVVSN | ||||||
Compositional bias | 214-231 | Polar residues | ||||
Sequence: SANNDEQNNDSTKPMTKS | ||||||
Compositional bias | 232-249 | Basic and acidic residues | ||||
Sequence: KRSSQQEESKEHNDLCQL | ||||||
Motif | 253-260 | Nuclear localization signal 2 | ||||
Sequence: TKKTPSRS | ||||||
Domain | 410-510 | Tudor; atypical | ||||
Sequence: YEQLVAYTGSVKKGDVIAYRLIELTSSWTPEVSSFRVGKISYYDPDSKMVTLMPVQEFPIEKKTEEDDDFCMQPDTSLYKEDGSLEIEFSALLDVRSVKTS | ||||||
Region | 513-585 | Disordered | ||||
Sequence: DSAEVAKSALPEPDQSAKKPKLSANKELQTPAKENGEVSPWEELSEALSAKKAALSQANNGWNKKGSSSGGSW | ||||||
Compositional bias | 564-585 | Polar residues | ||||
Sequence: KAALSQANNGWNKKGSSSGGSW |
Domain
Contains a N-terminal ordered domain (NOD) (1-117), central internal disordered domain (IDD) (118-349) and a C-terminal domain (CTD) (350-608) with an atypical Tudor domain containing probably two large unstructured loops.
Sequence similarities
Belongs to the coilin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length608
- Mass (Da)68,667
- Last updated2002-06-01 v1
- Checksum8734C1CA957CB27E
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 175-199 | Basic and acidic residues | ||||
Sequence: KRKKCKLDTTEESPDERENTAVVSN | ||||||
Compositional bias | 214-231 | Polar residues | ||||
Sequence: SANNDEQNNDSTKPMTKS | ||||||
Compositional bias | 232-249 | Basic and acidic residues | ||||
Sequence: KRSSQQEESKEHNDLCQL | ||||||
Compositional bias | 564-585 | Polar residues | ||||
Sequence: KAALSQANNGWNKKGSSSGGSW |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC007357 EMBL· GenBank· DDBJ | AAD31056.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002684 EMBL· GenBank· DDBJ | AEE28961.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY093028 EMBL· GenBank· DDBJ | AAM13027.1 EMBL· GenBank· DDBJ | mRNA | ||
AY128933 EMBL· GenBank· DDBJ | AAM91333.1 EMBL· GenBank· DDBJ | mRNA |