Q8RUS5 · ATG9_ARATH
- ProteinAutophagy-related protein 9
- GeneATG9
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids866 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion (PubMed:12114572, PubMed:24805779).
Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity).
In addition to autophagy, also plays a role in necrotic cell death (By similarity).
Plays an essential role in plant nutrient recycling (PubMed:12114572).
Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity).
In addition to autophagy, also plays a role in necrotic cell death (By similarity).
Plays an essential role in plant nutrient recycling (PubMed:12114572).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | phagophore assembly site membrane | |
Biological Process | autophagy | |
Biological Process | defense response to fungus | |
Biological Process | lipid transport |
Keywords
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAutophagy-related protein 9
- Short namesAtAPG9
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ8RUS5
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Preautophagosomal structure membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-94 | Cytoplasmic | ||||
Sequence: MMSSGHKGPNVRNFFKWQRGESSSSLTTGLLHNESHEIELSNYGGIPSPGSESPSGLLNGESLNVQPIADLDLFVERLYSYYRDKGLWCIIVKW | ||||||
Transmembrane | 95-115 | Helical | ||||
Sequence: AVELLSLGFIICFSGFFLLYV | ||||||
Topological domain | 116-153 | Lumenal | ||||
Sequence: DWNGLQNAKCGMDAVESGTKPCDLVKEAIHPHPLSPFT | ||||||
Transmembrane | 154-174 | Helical | ||||
Sequence: LTTAIIVGYLALFSVYWLFCF | ||||||
Topological domain | 175-319 | Cytoplasmic | ||||
Sequence: LRFFAQLKDTLDFRHFYYNNLHVTDNEILTMPWATVLEKVVQLQSSQCLCVVKDLSAHDMVMRLMRKENYLIGMLNKGLLSFPISHWIPGAGPAVKSAPDGTQYHLVLTKTLEWTLNWCILQSMFDCNFRVRRDFVSNPTTLKKR | ||||||
Intramembrane | 320-340 | |||||
Sequence: LFVVGLAMLLLSPFLVIFMLV | ||||||
Topological domain | 341-404 | Cytoplasmic | ||||
Sequence: YLFLRHAEQFYNHPSTASSRRWSNLSKWLFREFNEVDHLFKHRINSSVVHASEYLKQFPSPIIS | ||||||
Transmembrane | 405-425 | Helical | ||||
Sequence: IIAKFVSFVSGGFAAVLIIIA | ||||||
Topological domain | 426-433 | Lumenal | ||||
Sequence: FLEESLLE | ||||||
Transmembrane | 434-454 | Helical | ||||
Sequence: GHIFGRNLFWYAAVFGTITAI | ||||||
Topological domain | 455-507 | Cytoplasmic | ||||
Sequence: SRAAISDELLVLDPVGTMSLVVQNTHYMPKRWRGKENKDDVRLELETLFQYTG | ||||||
Intramembrane | 508-528 | |||||
Sequence: MMLLEEIASIFITPFLLMFVV | ||||||
Topological domain | 529-866 | Cytoplasmic | ||||
Sequence: PKRVDDILQFIKDFTVDIEGVGHVCSFSAFYFENHGNIKYGSPHNATRREQRSSQGKMEKSFLSFQSSYPSWESDSLGKQFLSNLRTFRDRKLHEINTRHSSPSRAWRESTNTPALYRDIPRNPLASGNHTDSMWLIDPDQRNHPYLLDWYYTSQAHNRTDHPIERANEILTANQNATDCWPPDLGIRGEDSRDLLNMEASTSGQFFRESILRHDQPEGEDSYGSQHPLDGRNQWWGRGNHSQISTAHPATTNSFIEPPDFINRYTAGNLLDNSWSRRSIEEEDEEEEELDWEENARRNLSRTTFMDDNDIEAGIDLHFDDVYSSRPQETSTSSTTLR |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mutant plants are hypersensitive to nitrogen or carbon starvation and show early bolting senescence.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 49 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434624 | 1-866 | Autophagy-related protein 9 | |||
Sequence: MMSSGHKGPNVRNFFKWQRGESSSSLTTGLLHNESHEIELSNYGGIPSPGSESPSGLLNGESLNVQPIADLDLFVERLYSYYRDKGLWCIIVKWAVELLSLGFIICFSGFFLLYVDWNGLQNAKCGMDAVESGTKPCDLVKEAIHPHPLSPFTLTTAIIVGYLALFSVYWLFCFLRFFAQLKDTLDFRHFYYNNLHVTDNEILTMPWATVLEKVVQLQSSQCLCVVKDLSAHDMVMRLMRKENYLIGMLNKGLLSFPISHWIPGAGPAVKSAPDGTQYHLVLTKTLEWTLNWCILQSMFDCNFRVRRDFVSNPTTLKKRLFVVGLAMLLLSPFLVIFMLVYLFLRHAEQFYNHPSTASSRRWSNLSKWLFREFNEVDHLFKHRINSSVVHASEYLKQFPSPIISIIAKFVSFVSGGFAAVLIIIAFLEESLLEGHIFGRNLFWYAAVFGTITAISRAAISDELLVLDPVGTMSLVVQNTHYMPKRWRGKENKDDVRLELETLFQYTGMMLLEEIASIFITPFLLMFVVPKRVDDILQFIKDFTVDIEGVGHVCSFSAFYFENHGNIKYGSPHNATRREQRSSQGKMEKSFLSFQSSYPSWESDSLGKQFLSNLRTFRDRKLHEINTRHSSPSRAWRESTNTPALYRDIPRNPLASGNHTDSMWLIDPDQRNHPYLLDWYYTSQAHNRTDHPIERANEILTANQNATDCWPPDLGIRGEDSRDLLNMEASTSGQFFRESILRHDQPEGEDSYGSQHPLDGRNQWWGRGNHSQISTAHPATTNSFIEPPDFINRYTAGNLLDNSWSRRSIEEEDEEEEELDWEENARRNLSRTTFMDDNDIEAGIDLHFDDVYSSRPQETSTSSTTLR |
Proteomic databases
Expression
Tissue specificity
Expressed in roots, leaves, stems and flowers.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 744-781 | Disordered | ||||
Sequence: QPEGEDSYGSQHPLDGRNQWWGRGNHSQISTAHPATTN | ||||||
Compositional bias | 764-781 | Polar residues | ||||
Sequence: WGRGNHSQISTAHPATTN |
Domain
Forms a homotrimer with a solvated central pore, which is connected laterally to the cytosol through the cavity within each protomer. Acts as a lipid scramblase that uses its central pore to function: the central pore opens laterally to accommodate lipid headgroups, thereby enabling lipid flipping and redistribution of lipids added to the outer leaflet of ATG9-containing vesicles, thereby enabling growth into autophagosomes.
Sequence similarities
Belongs to the ATG9 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length866
- Mass (Da)99,467
- Last updated2002-06-01 v1
- Checksum8951A9F39911D340
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 764-781 | Polar residues | ||||
Sequence: WGRGNHSQISTAHPATTN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB073174 EMBL· GenBank· DDBJ | BAB88386.1 EMBL· GenBank· DDBJ | mRNA | ||
AC006593 EMBL· GenBank· DDBJ | AAD20671.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
CP002685 EMBL· GenBank· DDBJ | AEC08515.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY075619 EMBL· GenBank· DDBJ | AAL91630.1 EMBL· GenBank· DDBJ | mRNA | ||
AY099644 EMBL· GenBank· DDBJ | AAM20495.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000243 EMBL· GenBank· DDBJ | AAN15562.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000816 EMBL· GenBank· DDBJ | AAN33191.1 EMBL· GenBank· DDBJ | mRNA |