Q8RDN6 · NANA_FUSNN

Function

function

Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
6 mMN-acetylneuraminate
kcat is 12.5 sec-1.

Pathway

Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 1/5.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site44aceneuramate (UniProtKB | ChEBI)
Binding site45aceneuramate (UniProtKB | ChEBI)
Active site133Proton donor
Active site161Schiff-base intermediate with substrate
Binding site163aceneuramate (UniProtKB | ChEBI)
Binding site185aceneuramate (UniProtKB | ChEBI)
Binding site187aceneuramate (UniProtKB | ChEBI)
Binding site188aceneuramate (UniProtKB | ChEBI)
Binding site204aceneuramate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionN-acetylneuraminate lyase activity
Biological Processcarbohydrate metabolic process
Biological ProcessN-acetylneuraminate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    N-acetylneuraminate lyase
  • EC number
  • Short names
    NAL
    ; Neu5Ac lyase
  • Alternative names
    • N-acetylneuraminate pyruvate-lyase
    • N-acetylneuraminic acid aldolase
    • Sialate lyase
    • Sialic acid aldolase
    • Sialic acid lyase

Gene names

    • Name
      nanA
    • Ordered locus names
      FN1475

Organism names

Accessions

  • Primary accession
    Q8RDN6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001032131-290N-acetylneuraminate lyase

Proteomic databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the DapA family. NanA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    290
  • Mass (Da)
    32,803
  • Last updated
    2002-06-01 v1
  • Checksum
    DEE469A25A4075FC
MKGIYSALMVPYNEDGSINEKGLREIIRYNIDKMKVDGLYVGGSTGENFMISTEEKKRVFEIAIDEAKDSVNLIAQVGSINLNEAVELGKYVTKLGYKCLSAVTPFYYKFDFSEIKDYYETIVRETGNYMIIYSIPFLTGVNMSLSQFGELFENEKIIGVKFTAGDFYLLERVRKAFPDKLIFAGFDEMLLPATVLGVDGAIGSTYNINGIRAKQIFELAKNSKIDEALKIQHTTNDLIEGILSNGLYQTIKEILKLEGVDAGYCRKPMKKISQKQIEFAKELHKKFLKN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE009951
EMBL· GenBank· DDBJ
AAL95668.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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