Q8RDN6 · NANA_FUSNN
- ProteinN-acetylneuraminate lyase
- GenenanA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids290 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.
Catalytic activity
- aceneuramate = aldehydo-N-acetyl-D-mannosamine + pyruvate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
6 mM | N-acetylneuraminate |
kcat is 12.5 sec-1.
Pathway
Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 1/5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 44 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 45 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Active site | 133 | Proton donor | ||||
Sequence: Y | ||||||
Active site | 161 | Schiff-base intermediate with substrate | ||||
Sequence: K | ||||||
Binding site | 163 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 185 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 187 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 188 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 204 | aceneuramate (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | N-acetylneuraminate lyase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | N-acetylneuraminate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylneuraminate lyase
- EC number
- Short namesNAL ; Neu5Ac lyase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Fusobacteriota > Fusobacteriia > Fusobacteriales > Fusobacteriaceae > Fusobacterium
Accessions
- Primary accessionQ8RDN6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000103213 | 1-290 | N-acetylneuraminate lyase | |||
Sequence: MKGIYSALMVPYNEDGSINEKGLREIIRYNIDKMKVDGLYVGGSTGENFMISTEEKKRVFEIAIDEAKDSVNLIAQVGSINLNEAVELGKYVTKLGYKCLSAVTPFYYKFDFSEIKDYYETIVRETGNYMIIYSIPFLTGVNMSLSQFGELFENEKIIGVKFTAGDFYLLERVRKAFPDKLIFAGFDEMLLPATVLGVDGAIGSTYNINGIRAKQIFELAKNSKIDEALKIQHTTNDLIEGILSNGLYQTIKEILKLEGVDAGYCRKPMKKISQKQIEFAKELHKKFLKN |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length290
- Mass (Da)32,803
- Last updated2002-06-01 v1
- ChecksumDEE469A25A4075FC
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE009951 EMBL· GenBank· DDBJ | AAL95668.1 EMBL· GenBank· DDBJ | Genomic DNA |