Q8R5J9 · PRAF3_MOUSE

  • Protein
    PRA1 family protein 3
  • Gene
    Arl6ip5
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Regulates intracellular concentrations of taurine and glutamate (By similarity).
Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner (PubMed:12119102, PubMed:18684713).
Plays a role in the retention of SLC1A1/EAAC1 in the endoplasmic reticulum (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoskeleton
Cellular Componentendoplasmic reticulum membrane
Cellular Componentmembrane
Cellular Componentplasma membrane
Cellular Componentpresynaptic cytosol
Molecular Functiontransmembrane transporter binding
Biological Processcellular response to organic cyclic compound
Biological Processcellular response to oxidative stress
Biological Processglutathione metabolic process
Biological Processintrinsic apoptotic signaling pathway in response to oxidative stress
Biological ProcessL-glutamate import across plasma membrane
Biological ProcessL-glutamate transmembrane transport
Biological Processlearning or memory
Biological Processnegative regulation of L-glutamate import across plasma membrane
Biological Processnegative regulation of mitochondrial membrane potential
Biological Processnegative regulation of transport
Biological Processpositive regulation of apoptotic process
Biological Processpositive regulation of stress-activated MAPK cascade
Biological Processprotein localization to plasma membrane
Biological Processprotein transport
Biological Processregulation of neurotransmitter uptake

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    PRA1 family protein 3
  • Alternative names
    • ADP-ribosylation factor-like protein 6-interacting protein 5 (ARL-6-interacting protein 5; Aip-5)
    • Addicsin
    • GTRAP3-18
    • Glutamate transporter EAAC1-interacting protein
    • Prenylated Rab acceptor protein 2
    • Protein JWa

Gene names

    • Name
      Arl6ip5
    • Synonyms
      Aip5, Jwa, Pra2, Praf3

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • BALB/cJ
    • NOD
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8R5J9
  • Secondary accessions
    • Q8C2Q1
    • Q9D838
    • Q9DB37
    • Q9WUG9

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Multi-pass membrane protein
Cell membrane
; Multi-pass membrane protein
Cytoplasm
Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-39Cytoplasmic
Transmembrane40-60Helical
Transmembrane64-84Helical
Topological domain85-92Cytoplasmic
Transmembrane93-113Helical
Transmembrane115-135Helical
Topological domain136-188Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis110Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-112.
Mutagenesis112Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-110.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002208841-188PRA1 family protein 3

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the cerebral cortex, cerebellum, hippocampus, olfactory bulbs, medulla oblongate and limbic system (at protein level) (PubMed:18684713).
Ubiquitous

Induction

By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected. Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence (PubMed:12438930).
Was originally reported to be induced by retinoic acid (PubMed:12562531).

Gene expression databases

Interaction

Subunit

Homodimer. Heterodimer with ARL6IP1 (PubMed:18684713).
Forms multimers. Interacts with ARL6 (PubMed:10508919).
Interacts with prenylated RAB1A and RAB3A. Interacts with SLC1A1/EAAC1 (PubMed:12119102, PubMed:18684713).
Interacts with RTN2 (via first transmembrane domain) (By similarity).
Does not interact with VAMP1, VAMP2 or VAMP3 (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region103-117Required for homodimer formation and heterodimer formation with ARL6IP1
Region136-188Targeting to endoplasmic reticulum membrane

Sequence similarities

Belongs to the PRA1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    188
  • Mass (Da)
    21,558
  • Last updated
    2005-01-04 v2
  • Checksum
    5A679FC5071319F0
MDVNLAPLRAWDDFFPGSDRFARPDFRDISKWNNRVVSNLLYYQTNYLVVAAMMISVVGFLSPFNMILGGVIVVLVFMGFVWAAHNKDILRRMKKQYPTAFVMVVMLASYFLISMFGGVMVFVFGITLPLLLMFIHASLRLRNLKNKLENKMEGIGLKKTPMGIILDALEQQEDNINKFADYISKARE

Sequence caution

The sequence AAD33050.1 differs from that shown. Reason: Erroneous translation Wrong choice of frame.
The sequence AAD33050.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict11in Ref. 4; BAC40209
Sequence conflict14in Ref. 4; BAC40209
Sequence conflict19in Ref. 6
Sequence conflict86in Ref. 6
Sequence conflict103in Ref. 4; BAB25717
Sequence conflict117in Ref. 6
Sequence conflict140in Ref. 4; BAB25717
Sequence conflict163in Ref. 1; AAL77876
Sequence conflict178in Ref. 4; BAC40209

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF421860
EMBL· GenBank· DDBJ
AAL77876.1
EMBL· GenBank· DDBJ
mRNA
D87211
EMBL· GenBank· DDBJ
BAC24103.1
EMBL· GenBank· DDBJ
mRNA
AF265214
EMBL· GenBank· DDBJ
AAL74056.1
EMBL· GenBank· DDBJ
mRNA
AK005259
EMBL· GenBank· DDBJ
BAB23912.1
EMBL· GenBank· DDBJ
mRNA
AK008519
EMBL· GenBank· DDBJ
BAB25717.1
EMBL· GenBank· DDBJ
mRNA
AK088205
EMBL· GenBank· DDBJ
BAC40209.1
EMBL· GenBank· DDBJ
mRNA
AK076519
EMBL· GenBank· DDBJ
BAC36376.1
EMBL· GenBank· DDBJ
mRNA
BC003897
EMBL· GenBank· DDBJ
AAH03897.1
EMBL· GenBank· DDBJ
mRNA
AF133912
EMBL· GenBank· DDBJ
AAD33050.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

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