Q8R4U9 · SGK2_RAT
- ProteinSerine/threonine-protein kinase Sgk2
- GeneSgk2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids367 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na+ channels: SCNN1A/ENAC, K+ channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Activity regulation
Two specific sites, one in the kinase domain (Thr-193) and the other in the C-terminal regulatory region (Ser-356), need to be phosphorylated for its full activation.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleoplasm | |
Molecular Function | ATP binding | |
Molecular Function | potassium channel regulator activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | intracellular signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase Sgk2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ8R4U9
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086648 | 1-367 | Serine/threonine-protein kinase Sgk2 | |||
Sequence: MASSPVGVPSPQPSRANGNINLGPSANPNARPTDFDFLKVIGKGNYGKVLLAKRKSDGAFYAVKVLQKKSILKNKEQSHIMAERNVLLKNVRHPFLVGLRYSFQTPEKLYFVLDYVNGGELFFHLQREHRFLEPRARFYTAEVASAIGYLHSLNIIYRDLKPENILLDCQGHVVLTDFGLCKECVEPEETTSTFCGTPEYLAPEVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFFNTDVAQMYENILHQPLQIPGGRTVAACDLLQGLLHKDQRQRLGSKEDFLDIKNHMFFSPINWDDLYHKRLTPPFNPNVEGPADLKHFDPEFTQEAVSKSIGCTPDTMSSSSGASSAFLGFSYAQDDDDILDS | ||||||
Modified residue | 10 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 193 | Phosphothreonine; by PDPK1 | ||||
Sequence: T | ||||||
Modified residue | 334 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 356 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 357 | Phosphotyrosine | ||||
Sequence: Y |
Post-translational modification
Activated by phosphorylation on Ser-356 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-193.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the proximal tubule and thick ascending limb of the loop of Henle (TALH).
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-28 | Disordered | ||||
Sequence: MASSPVGVPSPQPSRANGNINLGPSANP | ||||||
Domain | 35-292 | Protein kinase | ||||
Sequence: FDFLKVIGKGNYGKVLLAKRKSDGAFYAVKVLQKKSILKNKEQSHIMAERNVLLKNVRHPFLVGLRYSFQTPEKLYFVLDYVNGGELFFHLQREHRFLEPRARFYTAEVASAIGYLHSLNIIYRDLKPENILLDCQGHVVLTDFGLCKECVEPEETTSTFCGTPEYLAPEVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFFNTDVAQMYENILHQPLQIPGGRTVAACDLLQGLLHKDQRQRLGSKEDFLDIKNHMFF | ||||||
Motif | 68-77 | Nuclear localization signal | ||||
Sequence: KKSILKNKEQ | ||||||
Domain | 293-367 | AGC-kinase C-terminal | ||||
Sequence: SPINWDDLYHKRLTPPFNPNVEGPADLKHFDPEFTQEAVSKSIGCTPDTMSSSSGASSAFLGFSYAQDDDDILDS |
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length367
- Mass (Da)41,361
- Last updated2006-12-12 v2
- Checksum49F2231445645B50
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6A9X9 | A0A8I6A9X9_RAT | Sgk2 | 338 | ||
R9PXX7 | R9PXX7_RAT | Sgk2 | 361 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AABR03025367 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AF361756 EMBL· GenBank· DDBJ | AAL91351.1 EMBL· GenBank· DDBJ | mRNA |