Q8R426 · KCIP1_RAT
- ProteinA-type potassium channel modulatory protein KCNIP1
- GeneKcnip1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids227 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels (PubMed:14980206).
Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:14980206).
Modulates KCND2/Kv4.2 currents (PubMed:14980206).
In vitro, modulates KCND1/Kv4.1 currents (By similarity).
Increases the presence of KCND2 at the cell surface (PubMed:14980206).
Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:14980206).
Modulates KCND2/Kv4.2 currents (PubMed:14980206).
In vitro, modulates KCND1/Kv4.1 currents (By similarity).
Increases the presence of KCND2 at the cell surface (PubMed:14980206).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 146 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 148 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 150 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 152 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 157 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 194 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 196 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 198 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 205 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic side of plasma membrane | |
Cellular Component | dendrite | |
Cellular Component | neuronal cell body | |
Cellular Component | potassium channel complex | |
Cellular Component | voltage-gated potassium channel complex | |
Molecular Function | calcium ion binding | |
Molecular Function | potassium channel activity | |
Molecular Function | potassium channel regulator activity | |
Molecular Function | protein-containing complex binding | |
Molecular Function | transmembrane transporter binding | |
Biological Process | positive regulation of action potential | |
Biological Process | regulation of potassium ion transmembrane transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameA-type potassium channel modulatory protein KCNIP1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ8R426
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 214-227 | Abolishes interaction with KCND2. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000073820 | 1-227 | A-type potassium channel modulatory protein KCNIP1 | |||
Sequence: MGAVMGTFSSLQTKQRRPSKDIAWWYYQYQRDKIEDDLEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEETFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM |
Proteomic databases
Expression
Tissue specificity
Interaction
Subunit
Component of heteromultimeric potassium channels (PubMed:14980206, PubMed:15356203).
Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity).
Part of a heterooctamer composed of the tetrameric channel and four KCNIP1 chains (By similarity).
Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. Self-associates to form homodimers and homotetramers. Interacts with KCNIP2 isoform 3 in a calcium-dependent manner (By similarity).
Interacts with KCND2; this interaction mediates the capture of both the N- and C-terminus of KCND2, thus preventing KCND2 N-type inactivation and modulates the channel gating kinetics (PubMed:10676964, PubMed:11423117, PubMed:14980206).
Interacts with KCND3; each KCNIP1 monomer interacts with two adjacent KCND3 subunits, through both the N-terminal inactivation ball of a KCND3 subunit and a C-terminal helix from the adjacent KCND3 subunit, clamping them together; this interaction stabilizes the tetrameric form and modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation (PubMed:10676964).
Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity).
Part of a heterooctamer composed of the tetrameric channel and four KCNIP1 chains (By similarity).
Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. Self-associates to form homodimers and homotetramers. Interacts with KCNIP2 isoform 3 in a calcium-dependent manner (By similarity).
Interacts with KCND2; this interaction mediates the capture of both the N- and C-terminus of KCND2, thus preventing KCND2 N-type inactivation and modulates the channel gating kinetics (PubMed:10676964, PubMed:11423117, PubMed:14980206).
Interacts with KCND3; each KCNIP1 monomer interacts with two adjacent KCND3 subunits, through both the N-terminal inactivation ball of a KCND3 subunit and a C-terminal helix from the adjacent KCND3 subunit, clamping them together; this interaction stabilizes the tetrameric form and modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation (PubMed:10676964).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 38-94 | EF-hand 1; degenerate | ||||
Sequence: LEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEETFKQIYAQFFP | ||||||
Domain | 97-132 | EF-hand 2 | ||||
Sequence: DASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRG | ||||||
Domain | 133-168 | EF-hand 3 | ||||
Sequence: TVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDM | ||||||
Domain | 181-216 | EF-hand 4 | ||||
Sequence: TPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNI | ||||||
Region | 214-227 | Interaction with KCND2 | ||||
Sequence: DNIMRSLQLFQNVM |
Sequence similarities
Belongs to the recoverin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q8R426-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsKChIP1b
- Length227
- Mass (Da)26,817
- Last updated2005-08-16 v2
- Checksum92A2F0D72A120197
Q8R426-2
- Name2
- SynonymsKChIP1a
- Differences from canonical
- 21-31: Missing
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2K0U3 | A0A0G2K0U3_RAT | Kcnip1 | 232 | ||
A0A8I6ALG6 | A0A8I6ALG6_RAT | Kcnip1 | 225 | ||
A0A0G2K701 | A0A0G2K701_RAT | Kcnip1 | 203 | ||
A0A0H2UHE0 | A0A0H2UHE0_RAT | Kcnip1 | 236 |
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 10 | in Ref. 3; AAN34942 | ||||
Sequence: S → P | ||||||
Alternative sequence | VSP_015048 | 21-31 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 37 | in Ref. 1; BAB03308 | ||||
Sequence: D → E | ||||||
Sequence conflict | 107 | in Ref. 2; AAL92564/AAL92565 | ||||
Sequence: N → D |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB046443 EMBL· GenBank· DDBJ | BAB03308.1 EMBL· GenBank· DDBJ | mRNA | ||
AY082657 EMBL· GenBank· DDBJ | AAL92564.1 EMBL· GenBank· DDBJ | mRNA | ||
AY082658 EMBL· GenBank· DDBJ | AAL92565.1 EMBL· GenBank· DDBJ | mRNA | ||
AY142709 EMBL· GenBank· DDBJ | AAN34942.1 EMBL· GenBank· DDBJ | mRNA |