Q8R3P2 · DTX2_MOUSE

  • Protein
    Probable E3 ubiquitin-protein ligase DTX2
  • Gene
    Dtx2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity).

Catalytic activity

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.
    EC:2.3.2.27 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnuclear membrane
Cellular Componentnucleoplasm
Molecular Functionubiquitin protein ligase activity
Molecular Functionzinc ion binding
Biological ProcessNotch signaling pathway
Biological Processprotein ubiquitination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable E3 ubiquitin-protein ligase DTX2
  • EC number
  • Alternative names
    • Protein deltex-2 (Deltex2; mDTX2)
    • RING-type E3 ubiquitin transferase DTX2

Gene names

    • Name
      Dtx2

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8R3P2
  • Secondary accessions
    • Q9CZV3
    • Q9ER07
    • Q9ER08

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: Predominantly cytoplasmic. Partially nuclear.

Keywords

Phenotypes & Variants

Involvement in disease

  • Recurrent site of retroviral integration in murine B-cell lymphomas

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 39 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002190841-619Probable E3 ubiquitin-protein ligase DTX2
Modified residue213Asymmetric dimethylarginine
Modified residue215Asymmetric dimethylarginine
Modified residue232Asymmetric dimethylarginine
Modified residue248N6-acetyllysine
Modified residue255Omega-N-methylarginine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in testis and the CNS.

Developmental stage

In the CNS, it is expressed in the developing neural tube starting from 10.5 dpc in the spinal cord and around 11.5 dpc in the telencephalon. Expressed ubiquitously throughout the spinal cord and telencephalon during neurogenesis. Expressed throughout the developing retina at 15.5 dpc. Not expressed in the somite or presomite during somitogenesis. Expressed slightly earlier that Dtx1 and Dtx3.

Gene expression databases

Interaction

Subunit

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1.

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, zinc finger.

TypeIDPosition(s)Description
Domain7-97WWE 1
Domain98-174WWE 2
Region243-271Disordered
Region283-328Disordered
Region355-389Disordered
Zinc finger409-470RING-type

Domain

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.

Sequence similarities

Belongs to the Deltex family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative splicing.

Q8R3P2-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    619
  • Mass (Da)
    67,195
  • Last updated
    2003-09-26 v2
  • Checksum
    A3A9F16F5B855697
MAMAPSSSLPQVYPSHVVVAVWEWQDGLGIWHPYSATVCSFIEQHFVRQRGQHFGLGSLAHSIPLGQADPSLAPYIIDLPSWTQFRQNTGTMRSVRRHLFSQNSAPGQGIVWEWLGDDGSWVAYEARICDYLEQQVARGIQVVDLAPLGYNYTVNYATLTQTNKTSSFCRSVRRQVGPVYPVTSDIAVPRQMGLICFCQQCLHGSGTGPVSGRYRHSMTNLPAYPAPQAPHRTTTVSGAHQAFAPYNKPSLSGARSAPRLNTTNPWAAAPPVAGNQSLFHSSLSHLGPQLLPSGPSTSSGASASFPSGPSSSSPGSAPTTVPVQMPKASRVQQALAGMTSVLSAIGLPVCLSRAPRPTGPPASRPASKSHSSVKRLRKMSVKEGAPKPEPEQVIRKYTEELKVAPEEDCIICMEKLAVASGYSDMTDSKALGPMVVGRLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPWGKMEVFRFQMSLPGHEDCGTILIVYNIPHGIQGPEHPSPGKPFTARGFPRQCYLPDSPQGRKVLELLKVAWKRRLIFTVGTSSTTGETDTVVWNEIHHKTEMDRNVTGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ

Q8R3P2-2

  • Name
    2
  • Synonyms
    DeltaE
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Q8R3P2-3

  • Name
    3
  • Note
    Splicing donor site not canonical.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 338-357: MTSVLSAIGLPVCLSRAPRP → EDRRVYWLLVLYSGYRELDN
    • 358-619: Missing

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0R4IZY1A0A0R4IZY1_MOUSEDtx2618
A0A1C7ZMZ6A0A1C7ZMZ6_MOUSEDtx28
A0A0G2JDR7A0A0G2JDR7_MOUSEDtx2151

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_008351337-382in isoform 2
Alternative sequenceVSP_008352338-357in isoform 3
Alternative sequenceVSP_008353358-619in isoform 3
Sequence conflict383in Ref. 1; BAB18940

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB015423
EMBL· GenBank· DDBJ
BAB18940.1
EMBL· GenBank· DDBJ
mRNA
AB015424
EMBL· GenBank· DDBJ
BAB18941.1
EMBL· GenBank· DDBJ
mRNA
AK012137
EMBL· GenBank· DDBJ
BAB28055.1
EMBL· GenBank· DDBJ
mRNA
AK044105
EMBL· GenBank· DDBJ
BAC31780.1
EMBL· GenBank· DDBJ
mRNA
AK054443
EMBL· GenBank· DDBJ
BAC35781.1
EMBL· GenBank· DDBJ
mRNA
BC024925
EMBL· GenBank· DDBJ
AAH24925.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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