Q8R0H9 · GGA1_MOUSE
- ProteinADP-ribosylation factor-binding protein GGA1
- GeneGga1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids635 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes (PubMed:25405894).
Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity).
Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894).
Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity).
Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity).
Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894).
Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | early endosome | |
Cellular Component | early endosome membrane | |
Cellular Component | nucleoplasm | |
Cellular Component | protein-containing complex | |
Cellular Component | trans-Golgi network | |
Molecular Function | phosphatidylinositol binding | |
Molecular Function | small GTPase binding | |
Molecular Function | ubiquitin binding | |
Biological Process | Golgi to plasma membrane protein transport | |
Biological Process | Golgi to plasma membrane transport | |
Biological Process | intracellular protein transport | |
Biological Process | positive regulation of protein catabolic process | |
Biological Process | protein catabolic process | |
Biological Process | protein localization | |
Biological Process | protein localization to cell surface | |
Biological Process | protein localization to ciliary membrane | |
Biological Process | retrograde transport, endosome to Golgi |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameADP-ribosylation factor-binding protein GGA1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8R0H9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein
Endosome membrane ; Peripheral membrane protein
Early endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000212681 | 1-635 | ADP-ribosylation factor-binding protein GGA1 | |||
Sequence: MEPAMEPETLEARINRATNPLNKELNWASINSFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKSKILELLYSWTVCLPEEVKIAEAYQMLKKQGIVKSDPKLPEDAIFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGAASSSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQANDNLTQVINLYKQLVRGEEVNGDATASSIPGSTSALLDLSGLDLPPPGTTQPATPTRPGNQSSPEQLSASVSLLDDELMSLGLSDPTPPSGTSSDSVGWDNFQSSDGTESSVPPPAQAPSMDCRPPAQAPPPTSSGLDDLDLLGKTLMQQALPPEAQQVRWEKQQPAPRLTLRDLQSKSSSPSPGAASLLHTTSPEPPGPPPQATPTEFSLTSITVPLESIKPSSILPVTVYDQHGFRVLFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVRLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLIFTMGDQTYNEMGDVDQFPPPETWGSL | ||||||
Modified residue | 185 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 354 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 417 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Monomer. Interacts with GGA2 and GGA3 (By similarity).
Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6 (PubMed:11950392, PubMed:22522702).
Interacts with RABEP1 and RABGEF1 (PubMed:25405894).
Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (By similarity).
Interacts with EPN4. Interacts with CCDC91 (By similarity).
Interacts with HEATR5B/p200a (By similarity).
Interacts with SYNRG/gamma-synergin (By similarity).
Interacts (via GAE doamin) with NECAP1 and NECAP2 (By similarity).
Interacts (via GAE domain) with AFTPH/aftiphilin (By similarity).
Interacts with TSG101 and UBC. Interacts with RNF11. Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498' (By similarity).
Interacts with CNST (PubMed:19864490).
Interacts with ADRA2B (By similarity).
Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (PubMed:25405894).
Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6 (PubMed:11950392, PubMed:22522702).
Interacts with RABEP1 and RABGEF1 (PubMed:25405894).
Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (By similarity).
Interacts with EPN4. Interacts with CCDC91 (By similarity).
Interacts with HEATR5B/p200a (By similarity).
Interacts with SYNRG/gamma-synergin (By similarity).
Interacts (via GAE doamin) with NECAP1 and NECAP2 (By similarity).
Interacts (via GAE domain) with AFTPH/aftiphilin (By similarity).
Interacts with TSG101 and UBC. Interacts with RNF11. Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498' (By similarity).
Interacts with CNST (PubMed:19864490).
Interacts with ADRA2B (By similarity).
Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (PubMed:25405894).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q8R0H9 | Cnst Q8CBC4-3 | 2 | EBI-2616212, EBI-2615407 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, motif, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 17-147 | VHS | ||||
Sequence: ATNPLNKELNWASINSFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKSKILELLYSWTVCLPEEVKIAEAYQMLKKQGIVKS | ||||||
Region | 114-273 | Interaction with ARF3 | ||||
Sequence: KILELLYSWTVCLPEEVKIAEAYQMLKKQGIVKSDPKLPEDAIFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGAASSSSEDLMKELYQRCERMRPTLFRLASDTEDND | ||||||
Domain | 171-298 | GAT | ||||
Sequence: DEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGAASSSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQANDNLTQVINLYKQLVR | ||||||
Region | 299-505 | Unstructured hinge | ||||
Sequence: GEEVNGDATASSIPGSTSALLDLSGLDLPPPGTTQPATPTRPGNQSSPEQLSASVSLLDDELMSLGLSDPTPPSGTSSDSVGWDNFQSSDGTESSVPPPAQAPSMDCRPPAQAPPPTSSGLDDLDLLGKTLMQQALPPEAQQVRWEKQQPAPRLTLRDLQSKSSSPSPGAASLLHTTSPEPPGPPPQATPTEFSLTSITVPLESIKP | ||||||
Region | 305-349 | Disordered | ||||
Sequence: DATASSIPGSTSALLDLSGLDLPPPGTTQPATPTRPGNQSSPEQL | ||||||
Motif | 357-361 | Autoinhibitory | ||||
Sequence: DDELM | ||||||
Region | 362-422 | Disordered | ||||
Sequence: SLGLSDPTPPSGTSSDSVGWDNFQSSDGTESSVPPPAQAPSMDCRPPAQAPPPTSSGLDDL | ||||||
Compositional bias | 367-397 | Polar residues | ||||
Sequence: DPTPPSGTSSDSVGWDNFQSSDGTESSVPPP | ||||||
Compositional bias | 400-414 | Pro residues | ||||
Sequence: APSMDCRPPAQAPPP | ||||||
Compositional bias | 455-472 | Polar residues | ||||
Sequence: RDLQSKSSSPSPGAASLL | ||||||
Region | 455-490 | Disordered | ||||
Sequence: RDLQSKSSSPSPGAASLLHTTSPEPPGPPPQATPTE | ||||||
Domain | 506-627 | GAE | ||||
Sequence: SSILPVTVYDQHGFRVLFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVRLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLIFTMGDQTYNEMGDVDQFP |
Domain
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Sequence similarities
Belongs to the GGA protein family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length635
- Mass (Da)69,972
- Last updated2002-06-01 v1
- Checksum9F5F1C37F41ECCC6
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2R8VI72 | A0A2R8VI72_MOUSE | Gga1 | 363 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 367-397 | Polar residues | ||||
Sequence: DPTPPSGTSSDSVGWDNFQSSDGTESSVPPP | ||||||
Compositional bias | 400-414 | Pro residues | ||||
Sequence: APSMDCRPPAQAPPP | ||||||
Compositional bias | 455-472 | Polar residues | ||||
Sequence: RDLQSKSSSPSPGAASLL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK080881 EMBL· GenBank· DDBJ | BAC38058.1 EMBL· GenBank· DDBJ | mRNA | ||
AK155195 EMBL· GenBank· DDBJ | BAE33109.1 EMBL· GenBank· DDBJ | mRNA | ||
BC026802 EMBL· GenBank· DDBJ | AAH26802.1 EMBL· GenBank· DDBJ | mRNA |