Q8R0H9 · GGA1_MOUSE

  • Protein
    ADP-ribosylation factor-binding protein GGA1
  • Gene
    Gga1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes (PubMed:25405894).
Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity).
Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894).
Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentearly endosome
Cellular Componentearly endosome membrane
Cellular Componentnucleoplasm
Cellular Componentprotein-containing complex
Cellular Componenttrans-Golgi network
Molecular Functionphosphatidylinositol binding
Molecular Functionsmall GTPase binding
Molecular Functionubiquitin binding
Biological ProcessGolgi to plasma membrane protein transport
Biological ProcessGolgi to plasma membrane transport
Biological Processintracellular protein transport
Biological Processpositive regulation of protein catabolic process
Biological Processprotein catabolic process
Biological Processprotein localization
Biological Processprotein localization to cell surface
Biological Processprotein localization to ciliary membrane
Biological Processretrograde transport, endosome to Golgi

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ADP-ribosylation factor-binding protein GGA1
  • Alternative names
    • Gamma-adaptin-related protein 1
    • Golgi-localized, gamma ear-containing, ARF-binding protein 1

Gene names

    • Name
      Gga1

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • NOD
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8R0H9
  • Secondary accessions
    • Q3U2N1

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus, trans-Golgi network membrane
; Peripheral membrane protein
Endosome membrane
; Peripheral membrane protein
Early endosome membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002126811-635ADP-ribosylation factor-binding protein GGA1
Modified residue185Phosphoserine
Modified residue354Phosphoserine
Modified residue417Phosphoserine

Post-translational modification

Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Monomer. Interacts with GGA2 and GGA3 (By similarity).
Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6 (PubMed:11950392, PubMed:22522702).
Interacts with RABEP1 and RABGEF1 (PubMed:25405894).
Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (By similarity).
Interacts with EPN4. Interacts with CCDC91 (By similarity).
Interacts with HEATR5B/p200a (By similarity).
Interacts with SYNRG/gamma-synergin (By similarity).
Interacts (via GAE doamin) with NECAP1 and NECAP2 (By similarity).
Interacts (via GAE domain) with AFTPH/aftiphilin (By similarity).
Interacts with TSG101 and UBC. Interacts with RNF11. Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498' (By similarity).
Interacts with CNST (PubMed:19864490).
Interacts with ADRA2B (By similarity).
Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (PubMed:25405894).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q8R0H9Cnst Q8CBC4-32EBI-2616212, EBI-2615407

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain, region, motif, compositional bias.

TypeIDPosition(s)Description
Domain17-147VHS
Region114-273Interaction with ARF3
Domain171-298GAT
Region299-505Unstructured hinge
Region305-349Disordered
Motif357-361Autoinhibitory
Region362-422Disordered
Compositional bias367-397Polar residues
Compositional bias400-414Pro residues
Compositional bias455-472Polar residues
Region455-490Disordered
Domain506-627GAE

Domain

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.

Sequence similarities

Belongs to the GGA protein family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    635
  • Mass (Da)
    69,972
  • Last updated
    2002-06-01 v1
  • Checksum
    9F5F1C37F41ECCC6
MEPAMEPETLEARINRATNPLNKELNWASINSFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVLETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKSKILELLYSWTVCLPEEVKIAEAYQMLKKQGIVKSDPKLPEDAIFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEKISKRVNAIEEVNNNVKLLTEMVMSHSQGAASSSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEILQANDNLTQVINLYKQLVRGEEVNGDATASSIPGSTSALLDLSGLDLPPPGTTQPATPTRPGNQSSPEQLSASVSLLDDELMSLGLSDPTPPSGTSSDSVGWDNFQSSDGTESSVPPPAQAPSMDCRPPAQAPPPTSSGLDDLDLLGKTLMQQALPPEAQQVRWEKQQPAPRLTLRDLQSKSSSPSPGAASLLHTTSPEPPGPPPQATPTEFSLTSITVPLESIKPSSILPVTVYDQHGFRVLFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAVPKVMKVRLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLIFTMGDQTYNEMGDVDQFPPPETWGSL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2R8VI72A0A2R8VI72_MOUSEGga1363

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias367-397Polar residues
Compositional bias400-414Pro residues
Compositional bias455-472Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK080881
EMBL· GenBank· DDBJ
BAC38058.1
EMBL· GenBank· DDBJ
mRNA
AK155195
EMBL· GenBank· DDBJ
BAE33109.1
EMBL· GenBank· DDBJ
mRNA
BC026802
EMBL· GenBank· DDBJ
AAH26802.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp