Q8QZY3 · DPPA3_MOUSE

  • Protein
    Developmental pluripotency-associated protein 3
  • Gene
    Dppa3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromatin
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentfemale pronucleus
Cellular Componentmale pronucleus
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular Functionmethylated histone binding
Biological Processembryonic cleavage
Biological Processepigenetic programing of female pronucleus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Developmental pluripotency-associated protein 3
  • Alternative names
    • Compaction-associated protein 1
    • Primordial germ cell protein 7
    • Stella

Gene names

    • Name
      Dppa3
    • Synonyms
      Cap1p, Crg1, Pgc7

Organism names

  • Taxonomic identifier
  • Strains
    • 129/SvEv
    • KM
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q8QZY3
  • Secondary accessions
    • F6S6F5

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Cytoplasm
Note: Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono-laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.

Keywords

Phenotypes & Variants

Disruption phenotype

Null mutation result in apparently normal offspring. No effect on early gonadal PGCs or gross abnormalities in the development of gametes. However, females display severely reduced fertility despite ovulation of normal numbers of oocytes. Null mutation resulted in preimplantation development failure. Embryos rarely reached the blastocyst stage.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis44Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-46.
Mutagenesis46Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-44.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002392661-150Developmental pluripotency-associated protein 3

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes.

Developmental stage

Detected at 3.5 dpc (at protein level). Activated during the process of germ cell specification at 7 dpc.25, specifically in the founder population of lineage-restricted primordial germ cells (PGCs). Thereafter, expressed in the germ line until about 15.5 dpc in male and 13.5 dpc in female gonads. Expressed during blastocyst, morula and 4-cell embryo stages.

Gene expression databases

Interaction

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-26Basic and acidic residues
Region1-32Disordered
Region1-75Required for H3K9me2-binding
Region76-150Required to exclude TET3 from the maternal pronucleus

Domain

Mediates binding to H3K9me2 via N-terminal region, while ability to exclude TET3 from the maternal pronucleus requires the C-terminal part.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    150
  • Mass (Da)
    17,670
  • Last updated
    2002-06-01 v1
  • Checksum
    88B59E272F8FAD42
MEEPSEKVDPMKDPETPQKKDEEDALDDTDVLQPETLVKVMKKLTLNPGVKRSARRRSLRNRIAAVPVENKSEKIRREVQSAFPKRRVRTLLSVLKDPIAKMRRLVRIEQRQKRLEGNEFERDSEPFRCLCTFCHYQRWDPSENAKIGKN

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
K4DID3K4DID3_MOUSEDppa3141

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-26Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY082485
EMBL· GenBank· DDBJ
AAM03317.1
EMBL· GenBank· DDBJ
mRNA
AB072734
EMBL· GenBank· DDBJ
BAB86304.1
EMBL· GenBank· DDBJ
mRNA
AF490347
EMBL· GenBank· DDBJ
AAO84505.1
EMBL· GenBank· DDBJ
mRNA
AY134859
EMBL· GenBank· DDBJ
AAN12283.1
EMBL· GenBank· DDBJ
mRNA
AK135769
EMBL· GenBank· DDBJ
BAE22650.1
EMBL· GenBank· DDBJ
mRNA
AK136263
EMBL· GenBank· DDBJ
BAE22904.1
EMBL· GenBank· DDBJ
mRNA
AC158651
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC099433
EMBL· GenBank· DDBJ
AAH99433.1
EMBL· GenBank· DDBJ
mRNA
BC100331
EMBL· GenBank· DDBJ
AAI00332.1
EMBL· GenBank· DDBJ
mRNA
BC107340
EMBL· GenBank· DDBJ
AAI07341.1
EMBL· GenBank· DDBJ
mRNA
BC107341
EMBL· GenBank· DDBJ
AAI07342.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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