Q8QZY3 · DPPA3_MOUSE
- ProteinDevelopmental pluripotency-associated protein 3
- GeneDppa3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids150 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromatin | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | female pronucleus | |
Cellular Component | male pronucleus | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | methylated histone binding | |
Biological Process | embryonic cleavage | |
Biological Process | epigenetic programing of female pronucleus |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameDevelopmental pluripotency-associated protein 3
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ8QZY3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localized in the cytoplasm at the primary oocyte stage and in oocytes within mono-laminar follicles. Expressed in the nucleus and cytoplasm of oocytes in bi-laminar and Graafian follicles and during the 2-cell and morula stages. In 3.5 dpc blastocysts localization is mainly nuclear. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Null mutation result in apparently normal offspring. No effect on early gonadal PGCs or gross abnormalities in the development of gametes. However, females display severely reduced fertility despite ovulation of normal numbers of oocytes. Null mutation resulted in preimplantation development failure. Embryos rarely reached the blastocyst stage.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 44 | Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-46. | ||||
Sequence: L → A | ||||||
Mutagenesis | 46 | Abolishes localization to the nucleus and ability to prevent DNA demethylation; when associated with A-44. | ||||
Sequence: L → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000239266 | 1-150 | Developmental pluripotency-associated protein 3 | |||
Sequence: MEEPSEKVDPMKDPETPQKKDEEDALDDTDVLQPETLVKVMKKLTLNPGVKRSARRRSLRNRIAAVPVENKSEKIRREVQSAFPKRRVRTLLSVLKDPIAKMRRLVRIEQRQKRLEGNEFERDSEPFRCLCTFCHYQRWDPSENAKIGKN |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the immature oocytes and in newborn ovaries. Subsequently detected in maturing oocytes and in preimplantation embryos. Expressed in pluripotent embryonic but not in differentiated somatic cells. Expressed in blastocysts, epiblasts, primordial germ cells, embryonic gonads and primitive spermatogonia. No expression is detected in adult testes.
Developmental stage
Detected at 3.5 dpc (at protein level). Activated during the process of germ cell specification at 7 dpc.25, specifically in the founder population of lineage-restricted primordial germ cells (PGCs). Thereafter, expressed in the germ line until about 15.5 dpc in male and 13.5 dpc in female gonads. Expressed during blastocyst, morula and 4-cell embryo stages.
Gene expression databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-26 | Basic and acidic residues | ||||
Sequence: MEEPSEKVDPMKDPETPQKKDEEDAL | ||||||
Region | 1-32 | Disordered | ||||
Sequence: MEEPSEKVDPMKDPETPQKKDEEDALDDTDVL | ||||||
Region | 1-75 | Required for H3K9me2-binding | ||||
Sequence: MEEPSEKVDPMKDPETPQKKDEEDALDDTDVLQPETLVKVMKKLTLNPGVKRSARRRSLRNRIAAVPVENKSEKI | ||||||
Region | 76-150 | Required to exclude TET3 from the maternal pronucleus | ||||
Sequence: RREVQSAFPKRRVRTLLSVLKDPIAKMRRLVRIEQRQKRLEGNEFERDSEPFRCLCTFCHYQRWDPSENAKIGKN |
Domain
Mediates binding to H3K9me2 via N-terminal region, while ability to exclude TET3 from the maternal pronucleus requires the C-terminal part.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length150
- Mass (Da)17,670
- Last updated2002-06-01 v1
- Checksum88B59E272F8FAD42
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
K4DID3 | K4DID3_MOUSE | Dppa3 | 141 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-26 | Basic and acidic residues | ||||
Sequence: MEEPSEKVDPMKDPETPQKKDEEDAL |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY082485 EMBL· GenBank· DDBJ | AAM03317.1 EMBL· GenBank· DDBJ | mRNA | ||
AB072734 EMBL· GenBank· DDBJ | BAB86304.1 EMBL· GenBank· DDBJ | mRNA | ||
AF490347 EMBL· GenBank· DDBJ | AAO84505.1 EMBL· GenBank· DDBJ | mRNA | ||
AY134859 EMBL· GenBank· DDBJ | AAN12283.1 EMBL· GenBank· DDBJ | mRNA | ||
AK135769 EMBL· GenBank· DDBJ | BAE22650.1 EMBL· GenBank· DDBJ | mRNA | ||
AK136263 EMBL· GenBank· DDBJ | BAE22904.1 EMBL· GenBank· DDBJ | mRNA | ||
AC158651 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC099433 EMBL· GenBank· DDBJ | AAH99433.1 EMBL· GenBank· DDBJ | mRNA | ||
BC100331 EMBL· GenBank· DDBJ | AAI00332.1 EMBL· GenBank· DDBJ | mRNA | ||
BC107340 EMBL· GenBank· DDBJ | AAI07341.1 EMBL· GenBank· DDBJ | mRNA | ||
BC107341 EMBL· GenBank· DDBJ | AAI07342.1 EMBL· GenBank· DDBJ | mRNA |