Q8QMW9 · Q8QMW9_CWPXB
- ProteinCap-specific mRNA (nucleoside-2'-O-)-methyltransferase
- GeneCPXV106 CDS
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids333 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase OPG063 that creates mRNA's poly(A) tail. In the presence of OPG102, OPG063 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.
Catalytic activity
- a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 22 | mRNA (UniProtKB | ChEBI) | |||
Binding site | 39 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 66 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 68 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 72 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 95 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 97 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 116 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 138 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Active site | 175 | For methyltransferase activity | |||
Binding site | 177-180 | mRNA (UniProtKB | ChEBI) | |||
Binding site | 182 | mRNA (UniProtKB | ChEBI) | |||
Binding site | 205-207 | mRNA (UniProtKB | ChEBI) | |||
Binding site | 233 | mRNA (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | mRNA (nucleoside-2'-O-)-methyltransferase activity | |
Molecular Function | translation elongation factor activity | |
Biological Process | 7-methylguanosine mRNA capping | |
Biological Process | methylation | |
Biological Process | regulation of mRNA 3'-end processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCap-specific mRNA (nucleoside-2'-O-)-methyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Nucleocytoviricota > Pokkesviricetes > Chitovirales > Poxviridae > Chordopoxvirinae > Orthopoxvirus > Cowpox virus
- Virus hosts
Accessions
- Primary accessionQ8QMW9
Proteomes
Subcellular Location
Interaction
Subunit
Interacts with poly(A) polymerase catalytic subunit OPG063. Interacts with OPG109 and OPG123; these interactions might help linking transcription to capping and polyadenylation.
Structure
Sequence
- Sequence statusComplete
- Length333
- Mass (Da)38,841
- Last updated2002-06-01 v1
- Checksum6AAF96186F895261
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 305-324 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF482758 EMBL· GenBank· DDBJ | AAM13549.1 EMBL· GenBank· DDBJ | Genomic DNA |