Q8PHU9 · RUVC_XANAC

Function

function

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.

Catalytic activity

  • Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
    EC:3.1.21.10 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ion per subunit.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site8
Binding site8Mg2+ 1 (UniProtKB | ChEBI)
Active site69
Binding site69Mg2+ 2 (UniProtKB | ChEBI)
Active site141
Binding site141Mg2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentHolliday junction resolvase complex
Molecular Functioncrossover junction DNA endonuclease activity
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Crossover junction endodeoxyribonuclease RuvC
  • EC number
  • Alternative names
    • Holliday junction nuclease RuvC
    • Holliday junction resolvase RuvC

Gene names

    • Name
      ruvC
    • Ordered locus names
      XAC3150

Organism names

Accessions

  • Primary accession
    Q8PHU9

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001831501-174Crossover junction endodeoxyribonuclease RuvC

Interaction

Subunit

Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.

Structure

Family & Domains

Sequence similarities

Belongs to the RuvC family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    174
  • Mass (Da)
    18,732
  • Last updated
    2002-10-01 v1
  • Checksum
    12799C29EC55AA02
MTRILGIDPGSQRTGIGIIDIDEGGRSRHVHHAPLMLLGEGDFSQRLKRLLQGLGELIEIYRPDEVAIEKVFMGKSAASALKLGHARGAAICAVVMRDLPVHEYAAKEIKLALVGKGGADKVQVQHMVGIMLNLNGKLQPDAADALAVAITHAHVRATAQRLGVNTQQAWSRKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE008923
EMBL· GenBank· DDBJ
AAM37994.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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