Q8P3K2 · FUCD_XANCP
- ProteinL-fuconate dehydratase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids441 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.
Catalytic activity
- L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.03 mM | L-fuconate | |||||
6.2 mM | L-galactonate | |||||
2.1 mM | D-arabinonate |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22-24 | substrate | ||||
Sequence: GSD | ||||||
Binding site | 32 | substrate | ||||
Sequence: Y | ||||||
Binding site | 218 | substrate | ||||
Sequence: K | ||||||
Active site | 220 | Proton donor/acceptor | ||||
Sequence: K | ||||||
Binding site | 248 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 250 | substrate | ||||
Sequence: N | ||||||
Binding site | 274 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 274 | substrate | ||||
Sequence: E | ||||||
Binding site | 301 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 301 | substrate | ||||
Sequence: E | ||||||
Active site | 351 | |||||
Sequence: H | ||||||
Binding site | 351-353 | substrate | ||||
Sequence: HAG | ||||||
Binding site | 382 | substrate | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydro-lyase activity | |
Molecular Function | L-fuconate dehydratase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | carbohydrate catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-fuconate dehydratase
- EC number
- Short namesFucD
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae > Xanthomonas
Accessions
- Primary accessionQ8P3K2
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 220 | Inactive. | ||||
Sequence: K → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418813 | 1-441 | L-fuconate dehydratase | |||
Sequence: MRTIIALETHDVRFPTSRELDGSDAMNPDPDYSAAYVVLRTDGAEDLAGYGLVFTIGRGNDVQTAAVAALAEHVVGLSVDKVIADLGAFARRLTNDSQLRWLGPEKGVMHMAIGAVINAAWDLAARAANKPLWRFIAELTPEQLVDTIDFRYLSDALTRDEALAILRDAQPQRAARTATLIEQGYPAYTTSPGWLGYSDEKLVRLAKEAVADGFRTIKLKVGANVQDDIRRCRLARAAIGPDIAMAVDANQRWDVGPAIDWMRQLAEFDIAWIEEPTSPDDVLGHAAIRQGITPVPVSTGEHTQNRVVFKQLLQAGAVDLIQIDAARVGGVNENLAILLLAAKFGVRVFPHAGGVGLCELVQHLAMADFVAITGKMEDRAIEFVDHLHQHFLDPVRIQHGRYLAPEVPGFSAEMHPASIAEFSYPDGRFWVEDLAASKAKA |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length441
- Mass (Da)48,124
- Last updated2002-10-01 v1
- Checksum4BAF5BEC1D1E3327
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE008922 EMBL· GenBank· DDBJ | AAM43290.1 EMBL· GenBank· DDBJ | Genomic DNA |