Q8NRY8 · DLD_CORGL
- ProteinQuinone-dependent D-lactate dehydrogenase
- Genedld
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids571 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the oxidation of D-lactate to pyruvate. Has also weak activity with L-lactate and DL-2-hydroxybutyrate. Electrons derived from D-lactate oxidation enter the electron transport chain. Essential for growth with D-lactate as sole carbon and energy source.
Catalytic activity
- (R)-lactate + a quinone = a quinol + pyruvate
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.62 mM | D-lactate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
73.5 μmol/min/mg |
pH Dependence
Optimum pH is 7.0.
Temperature Dependence
Optimum temperature is 45 degrees Celsius.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78-82 | FAD (UniProtKB | ChEBI) | ||||
Sequence: ASNTG | ||||||
Binding site | 86-87 | FAD (UniProtKB | ChEBI) | ||||
Sequence: GS | ||||||
Binding site | 145 | FAD (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 152 | FAD (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 162 | FAD (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 263 | FAD (UniProtKB | ChEBI) | ||||
Sequence: V |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extrinsic component of cytoplasmic side of plasma membrane | |
Molecular Function | D-lactate dehydrogenase (quinone) activity | |
Molecular Function | FAD binding | |
Molecular Function | quinone binding | |
Biological Process | lactate oxidation | |
Biological Process | respiratory electron transport chain | |
Biological Process | transmembrane transport |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameQuinone-dependent D-lactate dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Corynebacteriaceae > Corynebacterium
Accessions
- Primary accessionQ8NRY8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Inactivation results in the loss of the ability to grow with D-lactate.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000441706 | 1-571 | Quinone-dependent D-lactate dehydrogenase | |||
Sequence: MTQPGQTTTTSHEAIDAFKRIVGDEHVLTSERATMPFSKGYRFGGGPVFAVVRPGTLVEMWRALQVSVDNNLIVIPQASNTGLTGGSGPGFQDYDRPIVIISTHRIDEVHLINDAREAISLAGTPLTHLTDALAKHQREPHSVIGSTSIGASVIGGIANNSGGSQIRKGPAFTREAIFARVNDDGKVELVNHLGISLGDDPEVALDRLQRGEWSPEDVTPAPEDSNETEYAEHLRKIVPSPARYNANPEYLFEASGSAGKLMVFAVRTRTFPREVHPTVFYIGTNNTHELEEIRRLFLEADMPLPISGEYMGRSAFDLAEKYGKDTFVFLKFMSPALQTRMFSFKTWANGLFSKIPGIGPTFADTVSQAMFSVLPNQLPKRMMEYRNRFEHHLLLTVSESQKAASEKMLKEFFAEPEHTGEFFICTSDEEKSASLNRFGAASAATRYAALKRRHIAGLIPIDVALRRDDWNWLEVLPEEIDDQLEVKAYYGHFFCHVMHQDYVAKQGVDLEALHDRIQHLLEERGAKLPAEHNYGRIYKLPESMEEHFKELDPTNTFNAGIGGTSPHKDWA |
Keywords
- PTM
Expression
Induction
Constitutively expressed.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 44-273 | FAD-binding PCMH-type | ||||
Sequence: GGGPVFAVVRPGTLVEMWRALQVSVDNNLIVIPQASNTGLTGGSGPGFQDYDRPIVIISTHRIDEVHLINDAREAISLAGTPLTHLTDALAKHQREPHSVIGSTSIGASVIGGIANNSGGSQIRKGPAFTREAIFARVNDDGKVELVNHLGISLGDDPEVALDRLQRGEWSPEDVTPAPEDSNETEYAEHLRKIVPSPARYNANPEYLFEASGSAGKLMVFAVRTRTFPR |
Sequence similarities
Belongs to the quinone-dependent D-lactate dehydrogenase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length571
- Mass (Da)63,723
- Last updated2002-10-01 v1
- Checksum55DE9D87A864264F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000036 EMBL· GenBank· DDBJ | BAB98294.1 EMBL· GenBank· DDBJ | Genomic DNA |