Q8NQ22 · SYA_CORGL

Function

function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site573Zn2+ (UniProtKB | ChEBI)
Binding site577Zn2+ (UniProtKB | ChEBI)
Binding site676Zn2+ (UniProtKB | ChEBI)
Binding site680Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionalanine-tRNA ligase activity
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processalanyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alanine--tRNA ligase
  • EC number
  • Alternative names
    • Alanyl-tRNA synthetase
      (AlaRS
      )

Gene names

    • Name
      alaS
    • Ordered locus names
      Cgl1632, cg1838

Organism names

Accessions

  • Primary accession
    Q8NQ22

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000750991-888Alanine--tRNA ligase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Domain

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    888
  • Mass (Da)
    96,226
  • Last updated
    2002-10-25 v1
  • Checksum
    18AE4DC95F5DDD59
MQTHEIRERFTNHFVNAGHQAVPSASLILDDPNLLFVNAGMVPFKPYFLGQQTPPFENGTATSIQKCVRTLDIEEVGITTRHNTFFQMAGNFSFGQYFKEGAITHAWGLLTGSVADGGFGLDPERLWVTVYLDDDEAAEIWEKKIGVPSERIQRLGMADNYWSMGVPGPCGPCSEIYYDRGEKYGKEGGPVADDNRYMEIWNLVFMEKERGQGIGKDNFDILGDLPKKNIDTGMGVERVACILQDVENVYETDLLRPVIDVAETLTGTKYGSDNTSDIRFRVIADHSRTGMMLILDGVTPGNEGRGYILRRLLRRIIRSARLLGATGETMEQFMNTIMDTMTPSYPEIADNRERIMRVAVTEERAFLKTLVSGTHLFEEAATSIKAAGSTKVAGAQAFALHDTYGFPIDLTLEMAAEAGLEVDVEGFDSLMAEQRSRAKADSQAKKHGHTDLSIYREWVDNNPTVFTGFEELDSQSKVLGLLSDGAKISEATEGQEVEVILDQSPLYAESGGQLGDRGQILLGDTVLDVHDVQKIGKKLWVHKALVANGGLAVGDEVVASVDKQWRHAARQAHTATHLIHAALRQVLGPTALQAGSMNKPGYLRFDFNYTEQLTPAQVEQIQAITNEAVDTDWAVNTVETSLEEAKAMGAMALFGENYGSTVRVVEIGGPFSMELCGGTHVAHSSQIGPVALLGESSIGSGVRRIEAYSGLNSFNYLSKERALAEGLASSLKAPSEELPERVAQLVDKLKAAEKEIEALHRQQLMAQTADLLNNAQEIGGVTTLLLRVKDNTNAGDLRTIATTLKDKLGDREGVLVIASDNAGKVPFVVAATKAAVARGAHSGNLVKLVGSYIDGRGGGKADLAQGSGANIAGLESAFGAVRAEIEAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000036
EMBL· GenBank· DDBJ
BAB99025.1
EMBL· GenBank· DDBJ
Genomic DNA
BX927152
EMBL· GenBank· DDBJ
CAF21641.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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